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Thank you for the response. I have another question about your CKII antibodies. Among those listed on the abcam catalog, which one can detect both alpha and alpha prime subnits on Western blot and with high titer. I also like to know how much cell extract (amount of total protein) need to be loaded/lane for detection of CK11 on western blot. Best Regards
Asked on Jan 20 2006
Thank you for your enquiry and patience. Under the conditions tested, which included WB detection at optimized dilutions using lysate from cells expressing endogenous amounts of the subunits, they were specific as specified. That is, ab10466, ab10468 and ab10470 react with the alpha subunit and ab10474 reacts with the alpha prime subunit. We would recommend loading 20-30 ug protein lysate. Please contact us again if you have any additional questions.
Answered on Jan 30 2006