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    csnk2a1-antibody-ab137788.pdf

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Cell Biology Cell Cycle Kinases/Phosphatases Other
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Anti-CSNK2A1 antibody (ab137788)

  • Datasheet
  • SDS
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Western blot - Anti-CSNK2A1 antibody (ab137788)
  • Immunocytochemistry/ Immunofluorescence - Anti-CSNK2A1 antibody (ab137788)
  • Western blot - Anti-CSNK2A1 antibody (ab137788)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSNK2A1 antibody (ab137788)
  • Western blot - Anti-CSNK2A1 antibody (ab137788)
  • Western blot - Anti-CSNK2A1 antibody (ab137788)

Key features and details

  • Rabbit polyclonal to CSNK2A1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
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Recombinant Human CSNK2A1 protein (ab85981)

View more associated products

Overview

  • Product name

    Anti-CSNK2A1 antibody
    See all CSNK2A1 primary antibodies
  • Description

    Rabbit polyclonal to CSNK2A1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow
  • Immunogen

    Recombinant fragment corresponding to Human CSNK2A1 aa 166-360.

  • Positive control

    • 293T, A431, H1299, HeLaS3, HepG2, Molt-4 and Raji cell lysates; Cal27 xenograft tissue.
  • General notes

     This product was previously labelled as CKII alpha

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Other
    • Cell Biology
    • Apoptosis
    • Intracellular
    • Kinases
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Raji whole cell lysate (ab30124)
    • A431 whole cell lysate (ab7909)
    • MOLT4 whole cell lysate (ab7912)
  • Recombinant Protein

    • Recombinant Human CSNK2A1 protein (ab85981)

Applications

Our Abpromise guarantee covers the use of ab137788 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 45 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/500.

Target

  • Function

    Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha and alpha' chains contain the catalytic site. Participates in Wnt signaling. CK2 phosphorylates 'Ser-392' of p53/TP53 following UV irradiation.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily.
    Contains 1 protein kinase domain.
  • Target information above from: UniProt accession P68400 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 432370 Chicken
    • Entrez Gene: 282419 Cow
    • Entrez Gene: 1457 Human
    • Entrez Gene: 12995 Mouse
    • Entrez Gene: 116549 Rat
    • Omim: 115440 Human
    • SwissProt: P21868 Chicken
    • SwissProt: P68399 Cow
    • SwissProt: P68400 Human
    • SwissProt: Q60737 Mouse
    • SwissProt: P19139 Rat
    • Unigene: 644056 Human
    • Unigene: 654675 Human
    • Unigene: 23692 Mouse
    • Unigene: 4231 Rat
    see all
  • Alternative names

    • Casein kinase 2 alpha 1 polypeptide antibody
    • Casein kinase II alpha 1 antibody
    • Casein kinase II alpha 1 subunit antibody
    • Casein kinase II alpha subunit antibody
    • Casein kinase II subunit alpha antibody
    • CK II alpha antibody
    • CK II antibody
    • CK2 alpha antibody
    • CK2 catalytic subunit alpha antibody
    • CK2A1 antibody
    • CKII antibody
    • CKIIalpha antibody
    • CSK21_HUMAN antibody
    • CSNK2A1 antibody
    see all

Images

  • Western blot - Anti-CSNK2A1 antibody (ab137788)
    Western blot - Anti-CSNK2A1 antibody (ab137788)
    All lanes : Anti-CSNK2A1 antibody (ab137788) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : H1299 cell lysate
    Lane 4 : HeLa S3 cell lysate
    Lane 5 : HepG2 cell lysate
    Lane 6 : MOLT4 cell lysate
    Lane 7 : Raji cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 45 kDa



    10% SDS PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-CSNK2A1 antibody (ab137788)
    Immunocytochemistry/ Immunofluorescence - Anti-CSNK2A1 antibody (ab137788)
  • Western blot - Anti-CSNK2A1 antibody (ab137788)
    Western blot - Anti-CSNK2A1 antibody (ab137788)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSNK2A1 antibody (ab137788)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSNK2A1 antibody (ab137788)

    Immunohistochemical analysis of paraffin embedded Cal27 xenograft tissue labelling CSNK2A1 using ab137788 at 1/100 dilution.

  • Western blot - Anti-CSNK2A1 antibody (ab137788)
    Western blot - Anti-CSNK2A1 antibody (ab137788)
  • Western blot - Anti-CSNK2A1 antibody (ab137788)
    Western blot - Anti-CSNK2A1 antibody (ab137788)

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab137788? Please let us know so that we can cite the reference in this datasheet.

    ab137788 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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