Anti-CSNK2A1 antibody (ab137788)
Key features and details
- Rabbit polyclonal to CSNK2A1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CSNK2A1 antibody
See all CSNK2A1 primary antibodies -
Description
Rabbit polyclonal to CSNK2A1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow
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Immunogen
Recombinant fragment corresponding to Human CSNK2A1 aa 166-360.
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Positive control
- 293T, A431, H1299, HeLaS3, HepG2, Molt-4 and Raji cell lysates; Cal27 xenograft tissue.
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General notes
This product was previously labelled as CKII alpha
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab137788 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 45 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/500. |
Target
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Function
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha and alpha' chains contain the catalytic site. Participates in Wnt signaling. CK2 phosphorylates 'Ser-392' of p53/TP53 following UV irradiation. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily.
Contains 1 protein kinase domain. - Information by UniProt
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Database links
- Entrez Gene: 432370 Chicken
- Entrez Gene: 282419 Cow
- Entrez Gene: 1457 Human
- Entrez Gene: 12995 Mouse
- Entrez Gene: 116549 Rat
- Omim: 115440 Human
- SwissProt: P21868 Chicken
- SwissProt: P68399 Cow
see all -
Alternative names
- Casein kinase 2 alpha 1 polypeptide antibody
- Casein kinase II alpha 1 antibody
- Casein kinase II alpha 1 subunit antibody
see all
Images
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Western blot - Anti-CSNK2A1 antibody (ab137788)All lanes : Anti-CSNK2A1 antibody (ab137788) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : A431 cell lysate
Lane 3 : H1299 cell lysate
Lane 4 : HeLa S3 cell lysate
Lane 5 : HepG2 cell lysate
Lane 6 : MOLT4 cell lysate
Lane 7 : Raji cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 45 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-CSNK2A1 antibody (ab137788) -
Western blot - Anti-CSNK2A1 antibody (ab137788) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSNK2A1 antibody (ab137788)Immunohistochemical analysis of paraffin embedded Cal27 xenograft tissue labelling CSNK2A1 using ab137788 at 1/100 dilution.
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Western blot - Anti-CSNK2A1 antibody (ab137788) -
Western blot - Anti-CSNK2A1 antibody (ab137788)
Protocols
References (0)
ab137788 has not yet been referenced specifically in any publications.
