Recombinant Anti-CSNK2A1 antibody [EP1963Y] (ab76040)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1963Y] to CSNK2A1
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-CSNK2A1 antibody [EP1963Y]
See all CSNK2A1 primary antibodies -
Description
Rabbit monoclonal [EP1963Y] to CSNK2A1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details
Unsuitable for: ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
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Positive control
- WB: HeLa and HCT116 cell lysate IHC-P Human breast carcinoma tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1963Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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KO cell pellets
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76040 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/20 - 1/50.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval is recommended. |
Notes |
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Flow Cyt (Intra)
1/20 - 1/50. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is recommended. |
Target
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Function
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha and alpha' chains contain the catalytic site. Participates in Wnt signaling. CK2 phosphorylates 'Ser-392' of p53/TP53 following UV irradiation. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily.
Contains 1 protein kinase domain. - Information by UniProt
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Database links
- Entrez Gene: 1457 Human
- Entrez Gene: 12995 Mouse
- Entrez Gene: 116549 Rat
- Omim: 115440 Human
- SwissProt: P68400 Human
- SwissProt: Q60737 Mouse
- SwissProt: P19139 Rat
- Unigene: 644056 Human
see all -
Alternative names
- Casein kinase 2 alpha 1 polypeptide antibody
- Casein kinase 2 alpha antibody
- Casein kinase II alpha 1 antibody
see all
Images
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All lanes : Anti-CSNK2A1 antibody [EP1963Y] (ab76040) at 1/1000 dilution
Lane 1 : Wild-type HCT116 cell lysate
Lane 2 : CSNK2A1 knockout HCT116 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 45 kDaLanes 1-2: Merged signal (red and green). Green - ab76040 observed at 45 kDa. Red - loading control ab8245 observed at 37 kDa.
ab76040 Anti-CSNK2A1 antibody [EP1963Y] was shown to specifically react with CSNK2A1 in wild-type HCT116 cells. Loss of signal was observed when knockout cell line ab266864 (knockout cell lysate ab257401) was used. Wild-type and CSNK2A1 knockout samples were subjected to SDS-PAGE. ab76040 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-CSNK2A1 antibody [EP1963Y] (ab76040) at 1/500 dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : CSNK2A1 knockout HAP1 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 45 kDaLanes 1 - 4: Merged signal (red and green). Green – ab76040 observed at 45 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab76040 was shown to specifically react with CSNK2A1 when CCSNK2A1 knockout samples were used. Wild-type and CSNK2A1 knockout samples were subjected to SDS-PAGE. ab76040 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSNK2A1 antibody [EP1963Y] (ab76040)
Immunohistochemical analysis of CSNK2A1 in paraffin embedded human breast carcinoma tissue using ab76040 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Anti-CSNK2A1 antibody [EP1963Y] (ab76040) at 1/1000 dilution + HeLa cell lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa -
Overlay histogram showing Jurkat cells stained with ab76040 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76040, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab76040 has been referenced in 6 publications.
- Ali R et al. ATM Regulated PTEN Degradation Is XIAP E3 Ubiquitin Ligase Mediated in p85a Deficient Cancer Cells and Influence Platinum Sensitivity. Cells 8:N/A (2019). PubMed: 31635307
- Jiang C et al. CSNK2A1 Promotes Gastric Cancer Invasion Through the PI3K-Akt-mTOR Signaling Pathway. Cancer Manag Res 11:10135-10143 (2019). PubMed: 31819646
- Cannon C et al. Therapeutic targeting of protein kinase CK2 gene expression in feline oral squamous cell carcinoma - a naturally occurring large animal model of head and neck cancer. Hum Gene Ther Clin Dev N/A:N/A (2017). PubMed: 28335614
- Kulej K et al. Time-resolved Global and Chromatin Proteomics during Herpes Simplex Virus Type 1 (HSV-1) Infection. Mol Cell Proteomics 16:S92-S107 (2017). PubMed: 28179408
- Kendall JJ et al. CK2 blockade causes MPNST cell apoptosis and promotes degradation of ß-catenin. Oncotarget 7:53191-53203 (2016). PubMed: 27448963
- Manni S et al. Protein Kinase CK2 Inhibition Down Modulates the NF-?B and STAT3 Survival Pathways, Enhances the Cellular Proteotoxic Stress and Synergistically Boosts the Cytotoxic Effect of Bortezomib on Multiple Myeloma and Mantle Cell Lymphoma Cells. PLoS One 8:e75280 (2013). WB, IHC-P ; Human . PubMed: 24086494