Overview

  • Product name

    Anti-CSNK2A1 (phospho T360 + S362) antibody
    See all CSNK2A1 primary antibodies
  • Description

    Rabbit polyclonal to CSNK2A1 (phospho T360 + S362)
  • Host species

    Rabbit
  • Specificity

    ab119410 detects endogenous levels of Human  CSNK2A1 only when phosphorylated at Threm=onine 360/Srine 362

  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human CSNK2A1 conjugated to keyhole limpet haemocyanin.
    Database link: NP_001894.2

  • Positive control

    • Recombinant proteins CSNK2A1 wt, CSNK2A1 T360A, CSNK2A1 S362A, CSNK2A1T360/S362A phoshorylated by ERK2
  • General notes

     This product was previously labelled as CKII alpha

     

Properties

Applications

Our Abpromise guarantee covers the use of ab119410 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 45 kDa).

Target

  • Function

    Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha and alpha' chains contain the catalytic site. Participates in Wnt signaling. CK2 phosphorylates 'Ser-392' of p53/TP53 following UV irradiation.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • Casein kinase 2 alpha 1 polypeptide antibody
    • Casein kinase II alpha 1 antibody
    • Casein kinase II alpha 1 subunit antibody
    • Casein kinase II alpha subunit antibody
    • Casein kinase II subunit alpha antibody
    • CK II alpha antibody
    • CK II antibody
    • CK2 alpha antibody
    • CK2 catalytic subunit alpha antibody
    • CK2A1 antibody
    • CKII antibody
    • CKIIalpha antibody
    • CSK21_HUMAN antibody
    • CSNK2A1 antibody
    see all

Images

  • All lanes : Anti-CSNK2A1 (phospho T360 + S362) antibody (ab119410) at 1/1000 dilution

    Lane 1 : CSNK2A1
    Lane 2 : CSNK2A1 + ERK2
    Lane 3 : CSNK2A1 T360A
    Lane 4 : CSNK2A1 T360A + ERK2
    Lane 5 : CSNK2A1 S362A
    Lane 6 : CSNK2A1 S362A + ERK2
    Lane 7 : CSNK2A1 T360/S362A
    Lane 8 : CSNK2A1 T360/S362A + ERK2

    Predicted band size: 45 kDa



    Lower panel stained with  CSNK2A1 antibody as a loading control.

  • Anti-CSNK2A1 (phospho T360 + S362) antibody (ab119410) at 1/1000 dilution + C57BL/6 Mouse splenocyte whole cell lysate at 40 µg

    Secondary
    HRP-conjugated goat anti-rabbit monoclonal IgG
    at 1/1000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 45 kDa
    Observed band size: 45 kDa


    Exposure time: 1 minute


    Blocked with 5% milk for 1 hour at 25°C

    See Abreview

References

ab119410 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (C57BL/6 Mouse splenocytes)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
40 µg
Specification
C57BL/6 Mouse splenocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Tomar Ghansah

Verified customer

Submitted Oct 05 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up