Product nameAnti-CSNK2A1 (phospho T360 + S362) antibody
See all CSNK2A1 primary antibodies
DescriptionRabbit polyclonal to CSNK2A1 (phospho T360 + S362)
ab119410 detects endogenous levels of Human CSNK2A1 only when phosphorylated at Threm=onine 360/Srine 362
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human CSNK2A1 conjugated to keyhole limpet haemocyanin.
Database link: NP_001894.2
- Recombinant proteins CSNK2A1 wt, CSNK2A1 T360A, CSNK2A1 S362A, CSNK2A1T360/S362A phoshorylated by ERK2
This product was previously labelled as CKII alpha
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
Note: (without Mg2+ and Ca2+)
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab119410 was purified by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab119410 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 45 kDa).|
FunctionCasein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha and alpha' chains contain the catalytic site. Participates in Wnt signaling. CK2 phosphorylates 'Ser-392' of p53/TP53 following UV irradiation.
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily.
Contains 1 protein kinase domain.
- Information by UniProt
- Casein kinase 2 alpha 1 polypeptide antibody
- Casein kinase II alpha 1 antibody
- Casein kinase II alpha 1 subunit antibody
All lanes : Anti-CSNK2A1 (phospho T360 + S362) antibody (ab119410) at 1/1000 dilution
Lane 1 : CSNK2A1
Lane 2 : CSNK2A1 + ERK2
Lane 3 : CSNK2A1 T360A
Lane 4 : CSNK2A1 T360A + ERK2
Lane 5 : CSNK2A1 S362A
Lane 6 : CSNK2A1 S362A + ERK2
Lane 7 : CSNK2A1 T360/S362A
Lane 8 : CSNK2A1 T360/S362A + ERK2
Predicted band size: 45 kDa
Lower panel stained with CSNK2A1 antibody as a loading control.
Anti-CSNK2A1 (phospho T360 + S362) antibody (ab119410) at 1/1000 dilution + C57BL/6 Mouse splenocyte whole cell lysate at 40 µg
HRP-conjugated goat anti-rabbit monoclonal IgG
at 1/1000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: 1 minute
Blocked with 5% milk for 1 hour at 25°C
ab119410 has not yet been referenced specifically in any publications.