Overview

  • Product name

  • Description

    Rabbit polyclonal to CSP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Xenopus laevis
  • Immunogen

    Synthetic peptide from the C terminus of Rat CSP, conjugated to KLH. The sequence is identical in bovine.

  • Positive control

    • Mouse and Rat brain tissue extracts

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituent: 50% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab90499 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 22 kDa.
IP Use a concentration of 0.5 µg/ml.

Target

  • Function

    May have an important role in presynaptic function. May be involved in calcium-dependent neurotransmitter release at nerve endings.
  • Tissue specificity

    Expressed in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain and heart.
  • Sequence similarities

    Contains 1 J domain.
  • Post-translational
    modifications

    Fatty acylated. Heavily palmitoylated in the cysteine string motif.
  • Cellular localization

    Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • CLN 4 antibody
    • CLN4 antibody
    • CLN4B antibody
    • CSP antibody
    • cysteine string protein alpha antibody
    • Cysteine string protein antibody
    • DJC5 antibody
    • DnaJ (Hsp40) homolog subfamily C member 5 antibody
    • DnaJ homolog subfamily C member 5 antibody
    • DNAJC 5 antibody
    • Dnajc5 antibody
    • DNAJC5A antibody
    • DNJC5_HUMAN antibody
    • NCL antibody
    see all

Images

  • Anti-CSP antibody (ab90499) at 1/2000 dilution + Mouse brain tissue extract

    Predicted band size: 22 kDa

References

This product has been referenced in:

  • Shirafuji T  et al. The Role of Cysteine String Protein a Phosphorylation at Serine 10 and 34 by Protein Kinase C? for Presynaptic Maintenance. J Neurosci 38:278-290 (2018). Read more (PubMed: 29167402) »
  • Shirafuji T  et al. Validation of Anti-CSPa, SNAP25, Tyrosine Hydroxylase, Ubiquitin, Cleaved Caspase 3, and pSer PKC Motif Antibodies for Utilization in Western Blotting. Acta Histochem Cytochem 50:177-180 (2017). Read more (PubMed: 29343881) »
See all 2 Publications for this product

Customer reviews and Q&As

Answer

Thank you for contacting Abcam.

Although the predicted molecular weight is 22kDa, a lot of proteins undergo post translational modifications that will increase the actual molecular weight observed. The band in the western blot is running closer to 31kDa that 45, therefore I would say that if you see a band anywhere between 22-32kDa, then it will be the correct band you are seeing.

If there is anything else I can help you with, please let me know.

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