Key features and details
- Rabbit polyclonal to CSP-C
- Suitable for: WB, Indirect ELISA, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CSP-C antibody
See all CSP-C primary antibodies
DescriptionRabbit polyclonal to CSP-C
SpecificityThis antibody detects endogenous levels of total GZMH protein.
Tested applicationsSuitable for: WB, Indirect ELISA, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human CSP-C (internal sequence).
- A549 cells and extracts from K562 cells
Previously labelled as Granzyme H.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Our Abpromise guarantee covers the use of ab69876 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000. Detects bands of approximately 21 and 54 kDa (predicted molecular weight: 27 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionCytotoxic chymotrypsin-like serine protease with preference for bulky and aromatic residues at the P1 position and acidic residues at the P3' and P4' sites. Probably necessary for target cell lysis in cell-mediated immune responses. Participates in the antiviral response via direct cleavage of several proteins essential for viral replication.
Tissue specificityConstituvely expressed in NK cells.
Sequence similaritiesBelongs to the peptidase S1 family. Granzyme subfamily.
Contains 1 peptidase S1 domain.
Cellular localizationCytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes.
- Information by UniProt
- Cathepsin G like 2 antibody
- Cathepsin G-like 2 antibody
- CCP X antibody
All lanes : Anti-CSP-C antibody (ab69876) at 1/500 dilution
Lane 1 : Extracts from K562 cells
Lane 2 : Extracts from K562 cells with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 27 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted?
Additional bands at: 54 kDa. We are unsure as to the identity of these extra bands.
Immunofluorescence analysis of A549 cells using ab69876 at 1/500-1/1000 dilution.
Left image un-treated.Right image treated with immunizing peptide.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab69876 has been referenced in 1 publication.
- Tang H et al. Granzyme H of cytotoxic lymphocytes is required for clearance of the hepatitis B virus through cleavage of the hepatitis B virus X protein. J Immunol 188:824-31 (2012). WB, ICC/IF ; Human . PubMed: 22156339