Anti-CSP-C antibody (ab69876)
Key features and details
- Rabbit polyclonal to CSP-C
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CSP-C antibody
See all CSP-C primary antibodies -
Description
Rabbit polyclonal to CSP-C -
Host species
Rabbit -
Specificity
This antibody detects endogenous levels of total GZMH protein. -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human CSP-C (internal sequence).
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Positive control
- A549 cells and extracts from K562 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab69876 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | ||
ICC/IF |
Notes |
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I-ELISA: 1/5000.
WB: 1/500 - 1/1000. Detects bands of approximately 21 and 54 kDa (predicted molecular weight: 27 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Cytotoxic chymotrypsin-like serine protease with preference for bulky and aromatic residues at the P1 position and acidic residues at the P3' and P4' sites. Probably necessary for target cell lysis in cell-mediated immune responses. Participates in the antiviral response via direct cleavage of several proteins essential for viral replication. -
Tissue specificity
Constituvely expressed in NK cells. -
Sequence similarities
Belongs to the peptidase S1 family. Granzyme subfamily.
Contains 1 peptidase S1 domain. -
Cellular localization
Cytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes. - Information by UniProt
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Database links
- Entrez Gene: 2999 Human
- Omim: 116831 Human
- SwissProt: P20718 Human
- Unigene: 348264 Human
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Alternative names
- Cathepsin G like 2 antibody
- Cathepsin G-like 2 antibody
- CCP X antibody
see all
Images
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All lanes : Anti-CSP-C antibody (ab69876) at 1/500 dilution
Lane 1 : Extracts from K562 cells
Lane 2 : Extracts from K562 cells with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 27 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted?
Additional bands at: 54 kDa. We are unsure as to the identity of these extra bands. -
Immunofluorescence analysis of A549 cells using ab69876 at 1/500-1/1000 dilution.
Left image un-treated.Right image treated with immunizing peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab69876 has been referenced in 1 publication.
- Tang H et al. Granzyme H of cytotoxic lymphocytes is required for clearance of the hepatitis B virus through cleavage of the hepatitis B virus X protein. J Immunol 188:824-31 (2012). WB, ICC/IF ; Human . PubMed: 22156339