Anti-CSRP2/Dlp1 antibody (ab65946)
Key features and details
- Goat polyclonal to CSRP2/Dlp1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-CSRP2/Dlp1 antibody
See all CSRP2/Dlp1 primary antibodies -
Description
Goat polyclonal to CSRP2/Dlp1 -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Horse, Cow, Opossum -
Immunogen
Synthetic peptide corresponding to Human CSRP2/Dlp1 aa 86-97 (internal sequence).
Sequence:C-ERLGIKPESVQP
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Positive control
- Recombinant Human CSRP2/Dlp1 protein (ab126663) can be used as a positive control in WB. Human heart and kidney lysates. Mouse and rat kidney lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab65946 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab65946 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 21 kDa).
A 1 hour primary incubation is recommended for this product. Approx 26kDa band observed in Human Heart and Kidney lysates and in Mouse and Rat Kidney lysates (calculated MW of 21.0kDa according to NP_001312.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Huber et al, J Biol Chem. 2000 Feb 25;275(8):5504-11; PMID: 10681529). |
IHC-P |
Use a concentration of 3 - 6 µg/ml.
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Notes |
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WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 21 kDa). A 1 hour primary incubation is recommended for this product. Approx 26kDa band observed in Human Heart and Kidney lysates and in Mouse and Rat Kidney lysates (calculated MW of 21.0kDa according to NP_001312.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Huber et al, J Biol Chem. 2000 Feb 25;275(8):5504-11; PMID: 10681529). |
IHC-P
Use a concentration of 3 - 6 µg/ml. |
Target
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Function
Drastically down-regulated in response to PDGF-BB or cell injury, that promote smooth muscle cell proliferation and dedifferentiation. Seems to play a role in the development of the embryonic vascular system. -
Tissue specificity
Highly expressed in the aorta, but not in heart and skeletal muscle. -
Sequence similarities
Contains 2 LIM zinc-binding domains. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 539381 Cow
- Entrez Gene: 1466 Human
- Entrez Gene: 13008 Mouse
- Entrez Gene: 29317 Rat
- Omim: 601871 Human
- SwissProt: Q32LE9 Cow
- SwissProt: Q16527 Human
- SwissProt: P97314 Mouse
see all -
Alternative names
- CRP2 antibody
- CSRP2 antibody
- CSRP2_HUMAN antibody
see all
Images
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab65946 has been referenced in 1 publication.
- Wang J et al. Exosomal miR-27a Derived from Gastric Cancer Cells Regulates the Transformation of Fibroblasts into Cancer-Associated Fibroblasts. Cell Physiol Biochem 49:869-883 (2018). PubMed: 30184548