RecombinantRabMAb

Recombinant Anti-CSRP3 antibody [EPR12615(B)] (ab172952)

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Western blot - Anti-CSRP3 antibody [EPR12615(B)] (ab172952)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSRP3 antibody [EPR12615(B)] (ab172952)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSRP3 antibody [EPR12615(B)] (ab172952)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSRP3 antibody [EPR12615(B)] (ab172952)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSRP3 antibody [EPR12615(B)] (ab172952)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSRP3 antibody [EPR12615(B)] (ab172952)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSRP3 antibody [EPR12615(B)] (ab172952)
  • Immunoprecipitation - Anti-CSRP3 antibody [EPR12615(B)] (ab172952)
  • Anti-CSRP3 antibody [EPR12615(B)] (ab172952)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12615(B)] to CSRP3
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-CSRP3 antibody [EPR12615(B)]
    See all CSRP3 primary antibodies

  • Description

    Rabbit monoclonal [EPR12615(B)] to CSRP3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow

  • Immunogen

    Synthetic peptide within Human CSRP3 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: P50461

  • Positive control

    • Fetal heart, human skeletal muscle, fetal muscle lysates, human heart tissue, human uterus tissue

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab172952 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for ICC/IF.

    • Target

    • Function

      Positive regulator of myogenesis. Plays a crucial and specific role in the organization of cytosolic structures in cardiomyocytes. Could play a role in mechanical stretch sensing. May be a scaffold protein that promotes the assembly of interacting proteins at Z-line structures. It is essential for calcineurin anchorage to the Z line. Required for stress-induced calcineurin-NFAT activation.

    • Tissue specificity

      Cardiac and slow-twitch skeletal muscles.

    • Involvement in disease

      Defects in CSRP3 are the cause of cardiomyopathy dilated type 1M (CMD1M) [MIM:607482]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
      Defects in CSRP3 are the cause of cardiomyopathy familial hypertrophic type 12 (CMH12) [MIM:612124]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.

    • Sequence similarities

      Contains 2 LIM zinc-binding domains.

    • Cellular localization

      Nucleus. Cytoplasm. Cytoplasm > cytoskeleton. Cytoplasm > myofibril > sarcomere > Z line. Mainly cytoplasmic (By similarity). In the nucleus it associates with the actin cytoskeleton (Potential). In the Z line, found associated with GLRX3.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • cardiac antibody
      • Cardiac LIM protein antibody
      • CLP antibody
      • CMD1M antibody
      • CMH12 antibody
      • CRP3 antibody
      • Csrp3 antibody
      • CSRP3_HUMAN antibody
      • Cysteine and glycine-rich protein 3 antibody
      • Cysteine rich protein 3 antibody
      • Cysteine-rich protein 3 antibody
      • LIM domain only 4 antibody
      • LIM domain protein antibody
      • LMO4 antibody
      • MLP antibody
      • Muscle LIM protein antibody
      see all

    Images

    • Western blot - Anti-CSRP3 antibody [EPR12615(B)] (ab172952)
      Western blot - Anti-CSRP3 antibody [EPR12615(B)] (ab172952)
      All lanes : Anti-CSRP3 antibody [EPR12615(B)] (ab172952) at 1/1000 dilution

      Lane 1 : Fetal heart
      Lane 2 : Human skeletal muscle
      Lane 3 : Fetal muscle

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit antibody at 1/2000 dilution

      Predicted band size: 21 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSRP3 antibody [EPR12615(B)] (ab172952)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSRP3 antibody [EPR12615(B)] (ab172952)

      Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling CSRP3 with ab172952 at 1/1000 dilution.

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSRP3 antibody [EPR12615(B)] (ab172952)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSRP3 antibody [EPR12615(B)] (ab172952)

      Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling CSRP3 with ab172592 at 1/1000 dilution.

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSRP3 antibody [EPR12615(B)] (ab172952)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSRP3 antibody [EPR12615(B)] (ab172952)

      ab172952 showing -ve staining in Human normal tonsil tissue.

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSRP3 antibody [EPR12615(B)] (ab172952)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSRP3 antibody [EPR12615(B)] (ab172952)

      ab172952 showing -ve staining in Human normal lung tissue.

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSRP3 antibody [EPR12615(B)] (ab172952)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSRP3 antibody [EPR12615(B)] (ab172952)

      ab172952 showing -ve staining in Human normal liver tissue.

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSRP3 antibody [EPR12615(B)] (ab172952)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSRP3 antibody [EPR12615(B)] (ab172952)

      ab172952 showing -ve staining in Human normal brain tissue.

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-CSRP3 antibody [EPR12615(B)] (ab172952)
      Immunoprecipitation - Anti-CSRP3 antibody [EPR12615(B)] (ab172952)

      Western blot analysis on immunoprecipitation pellet from (1) fetal heart lysate or (2) 1X PBS (negative control) using ab172952, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    • Anti-CSRP3 antibody [EPR12615(B)] (ab172952)
      Anti-CSRP3 antibody [EPR12615(B)] (ab172952)

    References (0)

    Publishing research using ab172952? Please let us know so that we can cite the reference in this datasheet.

    ab172952 has not yet been referenced specifically in any publications.

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