Overview

  • Product name
    Anti-CSRP3 antibody [EPR12616(B)]
    See all CSRP3 primary antibodies
  • Description
    Rabbit monoclonal [EPR12616(B)] to CSRP3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CSRP3 aa 150 to the C-terminus. The exact sequence is proprietary.
    Database link: P50461

  • Positive control
    • fetal heart and fetal muscle lysates, Human heart and Human skeletal muscle tissues
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab173301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 20 kDa (predicted molecular weight: 21 kDa).
IHC-P 1/100 - 1/250.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function
      Positive regulator of myogenesis. Plays a crucial and specific role in the organization of cytosolic structures in cardiomyocytes. Could play a role in mechanical stretch sensing. May be a scaffold protein that promotes the assembly of interacting proteins at Z-line structures. It is essential for calcineurin anchorage to the Z line. Required for stress-induced calcineurin-NFAT activation.
    • Tissue specificity
      Cardiac and slow-twitch skeletal muscles.
    • Involvement in disease
      Defects in CSRP3 are the cause of cardiomyopathy dilated type 1M (CMD1M) [MIM:607482]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
      Defects in CSRP3 are the cause of cardiomyopathy familial hypertrophic type 12 (CMH12) [MIM:612124]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    • Sequence similarities
      Contains 2 LIM zinc-binding domains.
    • Cellular localization
      Nucleus. Cytoplasm. Cytoplasm > cytoskeleton. Cytoplasm > myofibril > sarcomere > Z line. Mainly cytoplasmic (By similarity). In the nucleus it associates with the actin cytoskeleton (Potential). In the Z line, found associated with GLRX3.
    • Information by UniProt
    • Database links
    • Alternative names
      • cardiac antibody
      • Cardiac LIM protein antibody
      • CLP antibody
      • CMD1M antibody
      • CMH12 antibody
      • CRP3 antibody
      • Csrp3 antibody
      • CSRP3_HUMAN antibody
      • Cysteine and glycine-rich protein 3 antibody
      • Cysteine rich protein 3 antibody
      • Cysteine-rich protein 3 antibody
      • LIM domain only 4 antibody
      • LIM domain protein antibody
      • LMO4 antibody
      • MLP antibody
      • Muscle LIM protein antibody
      see all

    Images

    • All lanes : Anti-CSRP3 antibody [EPR12616(B)] (ab173301) at 1/1000 dilution

      Lane 1 : fetal heart lysate
      Lane 2 : fetal muscle lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 21 kDa

    • Immunoprecipitation. ab173301 at 1/1000 labeling CSRP3 immunoprecipitated from fetal heart lysate using ab173301 at 1/10.

      Lane 2: 1X PBS (negative control).

    • Immunohistochemical analysis of paraffin embedded Human heart tissue labeling CSRP3 with ab173301 at 1/100.

    • Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue labeling CSRP3 with ab173301 at 1/100.

    References

    ab173301 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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