Product nameAnti-CSRP3 antibody [EPR12616(B)]
See all CSRP3 primary antibodies
DescriptionRabbit monoclonal [EPR12616(B)] to CSRP3
Tested applicationsSuitable for: WB, IHC-P, IPmore details
Unsuitable for: ICC/IF
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CSRP3 aa 150 to the C-terminus. The exact sequence is proprietary.
Database link: P50461
- fetal heart and fetal muscle lysates, Human heart and Human skeletal muscle tissues
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab173301 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 20 kDa (predicted molecular weight: 21 kDa).|
|IHC-P||1/100 - 1/250.|
|IP||1/10 - 1/100.|
FunctionPositive regulator of myogenesis. Plays a crucial and specific role in the organization of cytosolic structures in cardiomyocytes. Could play a role in mechanical stretch sensing. May be a scaffold protein that promotes the assembly of interacting proteins at Z-line structures. It is essential for calcineurin anchorage to the Z line. Required for stress-induced calcineurin-NFAT activation.
Tissue specificityCardiac and slow-twitch skeletal muscles.
Involvement in diseaseDefects in CSRP3 are the cause of cardiomyopathy dilated type 1M (CMD1M) [MIM:607482]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in CSRP3 are the cause of cardiomyopathy familial hypertrophic type 12 (CMH12) [MIM:612124]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
Sequence similaritiesContains 2 LIM zinc-binding domains.
Cellular localizationNucleus. Cytoplasm. Cytoplasm > cytoskeleton. Cytoplasm > myofibril > sarcomere > Z line. Mainly cytoplasmic (By similarity). In the nucleus it associates with the actin cytoskeleton (Potential). In the Z line, found associated with GLRX3.
- Information by UniProt
- cardiac antibody
- Cardiac LIM protein antibody
- CLP antibody
All lanes : Anti-CSRP3 antibody [EPR12616(B)] (ab173301) at 1/1000 dilution
Lane 1 : fetal heart lysate
Lane 2 : fetal muscle lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 21 kDa
Immunoprecipitation. ab173301 at 1/1000 labeling CSRP3 immunoprecipitated from fetal heart lysate using ab173301 at 1/10.
Lane 2: 1X PBS (negative control).
Immunohistochemical analysis of paraffin embedded Human heart tissue labeling CSRP3 with ab173301 at 1/100.
Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue labeling CSRP3 with ab173301 at 1/100.
ab173301 has not yet been referenced specifically in any publications.