Recombinant
RabMAb

Anti-CstF-50 antibody [EPR12332] (ab173288)

Overview

  • Product name
    Anti-CstF-50 antibody [EPR12332]
    See all CstF-50 primary antibodies
  • Description
    Rabbit monoclonal [EPR12332] to CstF-50
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human CstF-50. The exact sequence is proprietary.
    Database link: Q05048

  • Positive control
    • 293T, HeLa, A549 and K562 cell lysates; Human cervical carcinoma and testis tissues; A549 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR12332
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab173288 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/100.
IHC-P 1/100 - 1/250.
WB 1/1000 - 1/10000. Predicted molecular weight: 48 kDa.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. May be responsible for the interaction of CSTF with other factors to form a stable complex on the pre-mRNA.
    • Sequence similarities
      Contains 6 WD repeats.
    • Domain
      The WD6 domain is required for interaction with BARD1. WD domains are responsible for interaction with CSTF3.
      N-terminus mediates homodimerization.
    • Post-translational
      modifications
      The N-terminus is blocked.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • CF 1 50 kDa subunit antibody
      • CF-1 50 kDa subunit antibody
      • Cleavage stimulation factor 50 kDa subunit antibody
      • Cleavage stimulation factor 50kDa subunit antibody
      • Cleavage stimulation factor antibody
      • Cleavage stimulation factor subunit 1 antibody
      • Cleavage stimulation factor, 3 prime pre RNA, subunit 1, 50 kD antibody
      • cleavage stimulation factor, 3' pre RNA, subunit 1, 50kD antibody
      • cleavage stimulation factor, 3' pre RNA, subunit 1, 50kDa antibody
      • CstF 50 antibody
      • CSTF 50 kDa subunit antibody
      • CstF-50 antibody
      • CSTF1 antibody
      • CSTF1_HUMAN antibody
      • CstFp50 antibody
      • OTTHUMP00000031332 antibody
      see all

    Images

    • All lanes : Anti-CstF-50 antibody [EPR12332] (ab173288) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : A549 cell lysate
      Lane 4 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 48 kDa

    • Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling CstF-50 with ab173288 at 1/100 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling CstF-50 with ab173288 at 1/100 dilution.

    • Immunofluorescent analysis of A549 cells labeling CstF-50 with ab173288 at 1/50 dilution.

    References

    ab173288 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab173288.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up