Anti-CstF-64 antibody (ab167554)

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Western blot - Anti-CstF-64 antibody (ab167554)

    Key features and details

    • Mouse polyclonal to CstF-64
    • Suitable for: WB
    • Reacts with: Recombinant fragment
    • Isotype: IgG

    Overview

    • Product name

      Anti-CstF-64 antibody
      See all CstF-64 primary antibodies

    • Description

      Mouse polyclonal to CstF-64

    • Host species

      Mouse

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Recombinant fragment
      Predicted to work with: Mouse, Cow, Human

    • Immunogen

      Recombinant full length protein corresponding to Human CstF-64 aa 1-577.
      Sequence:

      MAGLTVRDPAVDRSLRSVFVGNIPYEATEEQLKDIFSEVGPVVSFRLVYD RETGKPKGYGFCEYQDQETALSAMRNLNGREFSGRALRVDNAASEKNKEE LKSLGTGAPVIESPYGETISPEDAPESISKAVASLPPEQMFELMKQMKLC VQNSPQEARNMLLQNPQLAYALLQAQVVMRIVDPEIALKILHRQTNIPTL IAGNPQPVHGAGPGSGSNVSMNQQNPQAPQAQSLGGMHVNGAPPLMQASM QGGVPAPGQMPAAVTGPGPGSLAPGGGMQAQVGMPGSGPVSMERGQVPMQ DPRAAMQRGSLPANVPTPRGLLGDAPNDPRGGTLLSVTGEVEPRGYLGPP HQGPPMHHVPGHESRGPPPHELRGGPLPEPRPLMAEPRGPMLDQRGPPLD GRGGRDPRGIDARGMEARAMEARGLDARGLEARAMEARAMEARAMEARAM EARAMEVRGMEARGMDTRGPVPGPRGPIPSGMQGPSPINMGAVVPQGSRQ VPVMQGTGMQGASIQGGSQPGGFSPGQNQVTPQDHEKAALIMQVLQLTAD QIAMLPPEQRQSILILKEQIQKSTGAP


      Database link: NP_001316.1
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • Positive control

      • CstF-64 transfected 293T cell line lysate

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

    • Storage buffer

      pH: 7.4
      Constituent: 99% PBS
    • Concentration information loading...

    • Purity

      Protein A purified

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Applications

    Our Abpromise guarantee covers the use of ab167554 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB Use a concentration of 1 µg/ml. Predicted molecular weight: 61 kDa. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Target

    • Function

      One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. This subunit is directly involved in the binding to pre-mRNAs.

    • Sequence similarities

      Contains 1 RRM (RNA recognition motif) domain.

    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.

    • Cellular localization

      Nucleus. Localized with DDX1 in cleavage bodies.
    • Information by UniProt

    • Database links

      • Alternative names

        • betaCstF 64 variant 2 antibody
        • CF-1 64 kDa subunit antibody
        • CF1 64 kDa subunit antibody
        • Cleavage stimulation factor 2 antibody
        • Cleavage stimulation factor 64 kDa subunit antibody
        • Cleavage stimulation factor subunit 2 antibody
        • Cleavage stimulation factor, 3' pre RNA, subunit 2, 64kDa antibody
        • CSTF 2 antibody
        • CstF 64 antibody
        • CSTF 64 kDa subunit antibody
        • CstF-64 antibody
        • CSTF2 antibody
        • CSTF2_HUMAN antibody
        • MGC188397 antibody
        see all

      Images

      • Western blot - Anti-CstF-64 antibody (ab167554)
        Western blot - Anti-CstF-64 antibody (ab167554)
        All lanes : Anti-CstF-64 antibody (ab167554) at 1 µg/ml

        Lane 1 : CstF-64 transfected 293T cell line lysate
        Lane 2 : Non-transfected 293T cell line lysate

        Lysates/proteins at 15 µl per lane.

        Predicted band size: 61 kDa

      References (0)

      Publishing research using ab167554? Please let us know so that we can cite the reference in this datasheet.

      ab167554 has not yet been referenced specifically in any publications.

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