Anti-CstF-64 antibody (ab72297)
Key features and details
- Rabbit polyclonal to CstF-64
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CstF-64 antibody
See all CstF-64 primary antibodies -
Description
Rabbit polyclonal to CstF-64 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rabbit, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide corresponding to Human CstF-64 aa 375-425.
Database link: NP_001316.1 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab72297 was affinity purified using an epitope specific to CstF-64 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72297 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 61 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
Use at an assay dependent concentration.
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Notes |
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WB
1/2000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 61 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
Use at an assay dependent concentration. |
Target
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Function
One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. This subunit is directly involved in the binding to pre-mRNAs. -
Sequence similarities
Contains 1 RRM (RNA recognition motif) domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. Localized with DDX1 in cleavage bodies. - Information by UniProt
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Database links
- Entrez Gene: 282588 Cow
- Entrez Gene: 1478 Human
- Omim: 300907 Human
- SwissProt: P33240 Human
- Unigene: 132370 Human
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Alternative names
- betaCstF 64 variant 2 antibody
- CF-1 64 kDa subunit antibody
- CF1 64 kDa subunit antibody
see all
Images
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All lanes : Anti-CstF-64 antibody (ab72297) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 61 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?
Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling CstF-64 with ab72297 at 1/1000 (1µg/ml). Detection: DAB.
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IHC image of CstF-64 staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72297, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Detection of human CstF-64 by Immunoprecipitation using whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), and ab72297 at 3 µg/mg lysate. Subsequent WB detection was performed using ab72297 at 0.1 µg/ml. Detection was by chemiluminescence with an exposure time of 3 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab72297 has been referenced in 2 publications.
- Ko SH et al. Interference of DNAJB6/MRJ Isoform Switch by Morpholino Inhibits Replication of HIV-1 and RSV. Mol Ther Nucleic Acids 14:251-261 (2019). PubMed: 30641477
- Kim C et al. Regulation of post-translational protein arginine methylation during HeLa cell cycle. Biochim Biophys Acta 1800:977-85 (2010). WB ; Human . PubMed: 20541591