Overview

  • Product name
  • Description
    Goat polyclonal to CSTF3
  • Host species
    Goat
  • Specificity
    ab174677 is expected to recognize isoform 1 (NP_001317.1) only.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human CSTF3 aa 109-120 (internal sequence) (Cysteine residue).
    Sequence:

    RETKGKLPSYKE


    Database link: NP_001317

  • Positive control
    • HeLa nuclear lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.3
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, 99% Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab174677 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab174677 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 83 kDa).

Target

  • Relevance
    CSTF3 (cleavage stimulation factor subunit 3) is required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs and is part of the CSTF complex. The CSTF complex is composed of CSTF1 (50 kDa subunit), CSTF2 (64 kDa subunit) and CSTF3 (77 kDa subunit). CSTF3 functions as a homodimer and interacts directly with CSTF1 and CSTF2.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • CF 1 77 kDa subunit antibody
    • Cleavage stimulation factor 3' pre RNA subunit 3 antibody
    • Cleavage stimulation factor 77 kDa subunit antibody
    • Cleavage stimulation factor subunit 3 antibody
    • Cleavage stimulation factor subunit 3 isoform 1 antibody
    • CSTF 3 antibody
    • CSTF 77 antibody
    • CSTF 77 kDa subunit antibody
    • CSTF77 antibody
    • MGC117398 antibody
    • MGC43001 antibody
    • MGC75122 antibody
    see all

Images

  • Anti-CSTF3 antibody (ab174677) at 0.1 µg/ml + HeLa nuclear lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 83 kDa
    Observed band size: 75 kDa (why is the actual band size different from the predicted?)



    Primary incubation was 1 hour.

References

ab174677 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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