Anti-CSTF3 antibody (ab72299)
Key features and details
- Rabbit polyclonal to CSTF3
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CSTF3 antibody
See all CSTF3 primary antibodies -
Description
Rabbit polyclonal to CSTF3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Horse, Cow, Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide corresponding to Human CSTF3 (N terminal). Corresponding to a region between residue 1 and 50 of human CSTF3 (NP_001317.1)
Database link: Q12996 -
Positive control
- Whole cell lysates from HeLa and 293T cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72299 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/2000 - 1/10000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
CSTF3 (cleavage stimulation factor subunit 3) is required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs and is part of the CSTF complex. The CSTF complex is composed of CSTF1 (50 kDa subunit), CSTF2 (64 kDa subunit) and CSTF3 (77 kDa subunit). CSTF3 functions as a homodimer and interacts directly with CSTF1 and CSTF2. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 1479 Human
- Entrez Gene: 100174283 Orangutan
- Omim: 600367 Human
- SwissProt: Q12996 Human
- SwissProt: Q5RDW9 Orangutan
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Alternative names
- CF 1 77 kDa subunit antibody
- Cleavage stimulation factor 3' pre RNA subunit 3 antibody
- Cleavage stimulation factor 77 kDa subunit antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling CSTF3 with ab72299 at 1/200 (1µg/ml). Detection: DAB.
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All lanes : Anti-CSTF3 antibody (ab72299) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Predicted band size: 83 kDa
Observed band size: 83 kDa
Additional bands at: 150 kDa, 31 kDa, 50 kDa, 60 kDa. We are unsure as to the identity of these extra bands. -
Detection of Human CSTF3 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72299 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab72299 has not yet been referenced specifically in any publications.