Anti-CSTF3 antibody (ab74407)
Key features and details
- Rabbit polyclonal to CSTF3
- Suitable for: IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CSTF3 antibody
See all CSTF3 primary antibodies -
Description
Rabbit polyclonal to CSTF3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus -
Immunogen
Synthetic peptide within Human CSTF3 aa 650 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_001317.1
Database link: Q12996 -
Positive control
- Human prostate carcinoma and mouse teratoma tissue. Other suitable positive controls are: Breast Carcinoma, Colon Carcinoma, Linitis Plastica Stomach Cancer, Non-Small Cell Lung Cancer, Ovarian Carcinoma, Pancreatic Islet Cell Tumor, Small Cell Lung Cancer, Stomach Adenocarcinoma and Testicular Seminoma tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab74407 was affinity purified using an epitope specific to CSTF3 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab74407 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Notes |
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Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
ab74407 is likely to work with frozen sections.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Relevance
CSTF3 (cleavage stimulation factor subunit 3) is required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs and is part of the CSTF complex. The CSTF complex is composed of CSTF1 (50 kDa subunit), CSTF2 (64 kDa subunit) and CSTF3 (77 kDa subunit). CSTF3 functions as a homodimer and interacts directly with CSTF1 and CSTF2. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 475948 Dog
- Entrez Gene: 1479 Human
- Entrez Gene: 228410 Mouse
- Entrez Gene: 100174283 Orangutan
- Entrez Gene: 362178 Rat
- Omim: 600367 Human
- SwissProt: Q12996 Human
- SwissProt: Q99LI7 Mouse
see all -
Alternative names
- CF 1 77 kDa subunit antibody
- Cleavage stimulation factor 3' pre RNA subunit 3 antibody
- Cleavage stimulation factor 77 kDa subunit antibody
see all
Images
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ab74407, at 1/250 dilution, staining CSTF3 in formalin fixed, paraffin embedded human prostate carcinoma tissue by Immunohistochemistry. Detection: DAB staining.
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ab74407, at 1/250 dilution, staining CSTF3 in formalin fixed, paraffin embedded mouse teratoma tissue by Immunohistochemistry. Detection: DAB staining.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab74407 has not yet been referenced specifically in any publications.