Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3941] to CT A
- Suitable for: WB, IP, Flow Cyt, ICC/IF
- Reacts with: Human
Product nameAnti-CT A antibody [EPR3941]
See all CT A primary antibodies
DescriptionRabbit monoclonal [EPR3941] to CT A
Tested applicationsSuitable for: WB, IP, Flow Cyt, ICC/IFmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Human
corresponding to Human CT A aa 350-450.
Database link: P49585
- A549, K562, HeLa and HepG2 cell lysates; HeLa cells
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Previously labelled as PCYT1A.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab92440 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: 1/100 - 1/250.
IP: 1/10 - 1/100.
WB: 1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
Is unsuitable for IHC-P.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionControls phosphatidylcholine synthesis.
PathwayPhospholipid metabolism; phosphatidylcholine biosynthesis; phosphatidylcholine from phosphocholine: step 1/2.
Sequence similaritiesBelongs to the cytidylyltransferase family.
modificationsThe serine residues of the C-terminus are phosphorylated. The inactive soluble form is stabilized by phosphorylation, the active membrane bound form is promoted by anionic lipids or diacylglycerol, and is stabilized by dephosphorylation.
Cellular localizationCytoplasm > cytosol. Membrane. It can interconvert between an inactive cytosolic form and an active membrane-bound form.
- Information by UniProt
- CCT A antibody
- CCT alpha antibody
- CCT-alpha antibody
All lanes : Anti-CT A antibody [EPR3941] (ab92440) at 1/1000 dilution
Lane 1 : A549 cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 42 kDa
ab92440, at 1/100 dilution, staining CT A in HeLa cells by Immunofluorescence.
ab92440 has not yet been referenced specifically in any publications.