Key features and details
- Mouse monoclonal [4D8] to CTAG1B
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-CTAG1B antibody [4D8]
See all CTAG1B primary antibodies
DescriptionMouse monoclonal [4D8] to CTAG1B
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
- HEK293T cell lysate transfected with pCMV6-ENTRY CTAG1B cDNA; COS7 cells transiently transfected with pCMV6-ENTRY CTAG1B cDNA; Human pancreas tissue.
General notesDilute in PBS (pH7.3) before use.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 50% Glycerol, 48% PBS
Concentration information loading...
PurityProtein G purified
Purification notesab139339 is purified from TCS by affinity chromatography.
Our Abpromise guarantee covers the use of ab139339 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 18 kDa.|
|IHC-P||1/150. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Tissue specificityExpressed in testis and ovary and in a wide variety of cancers. Detected in uterine myometrium. Expressed from 18 weeks until birth in human fetal testis. In the adult testis, is strongly expressed in spermatogonia and in primary spermatocytes, but not in post-meiotic cells or in testicular somatic cells (at protein level).
Sequence similaritiesBelongs to the CTAG family.
- Information by UniProt
- CTAG1A antibody
- CTAG1B antibody
- Autoimmunogenic cancer/testis antigen NY ESO 1 antibody
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labelling CTAG1B with ab139339 at 1/150 dilution.
All lanes : Anti-CTAG1B antibody [4D8] (ab139339) at 1/2000 dilution
Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY CTAG1B cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 18 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC207790)
immunofluorescent analysis of COS7 cells transiently transfected with pCMV6-ENTRY CTAG1B, labelling CTAG1B with ab139339 at 1/100 dilution.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab139339 has not yet been referenced specifically in any publications.