Anti-CTAG1B antibody [4D8] (ab139339)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [4D8] (ab139339)](https://www.abcam.com/ps/products/139/ab139339/Images/ab139339-154659-anti-ctag1b-antibody-4d8-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [4D8] (ab139339)")
Key features and details
- Mouse monoclonal [4D8] to CTAG1B
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CTAG1B antibody [4D8]
See all CTAG1B primary antibodies -
Description
Mouse monoclonal [4D8] to CTAG1B -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length Human CTAG1B (amino acids 1-180) produced in HEK293T cells.
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Positive control
- HEK293T cell lysate transfected with pCMV6-ENTRY CTAG1B cDNA; COS7 cells transiently transfected with pCMV6-ENTRY CTAG1B cDNA; Human pancreas tissue.
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General notes
Dilute in PBS (pH7.3) before use.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 50% Glycerol, 48% PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
ab139339 is purified from TCS by affinity chromatography. -
Clonality
Monoclonal -
Clone number
4D8 -
Isotype
IgG1 -
Research areas
Associated products
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab139339 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000. Predicted molecular weight: 18 kDa. | |
| IHC-P | 1/150. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/100. |
Target
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Tissue specificity
Expressed in testis and ovary and in a wide variety of cancers. Detected in uterine myometrium. Expressed from 18 weeks until birth in human fetal testis. In the adult testis, is strongly expressed in spermatogonia and in primary spermatocytes, but not in post-meiotic cells or in testicular somatic cells (at protein level). -
Sequence similarities
Belongs to the CTAG family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 1485 Human
- Entrez Gene: 246100 Human
- Omim: 300156 Human
- SwissProt: P78358 Human
- Unigene: 534310 Human
- Unigene: 729551 Human
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Alternative names
- CTAG1A antibody
- CTAG1B antibody
- Autoimmunogenic cancer/testis antigen NY ESO 1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [4D8] (ab139339)Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labelling CTAG1B with ab139339 at 1/150 dilution.
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![Western blot - Anti-CTAG1B antibody [4D8] (ab139339)](https://www.abcam.com/ps/products/139/ab139339/Images/ab139339-154661-anti-ctag1b-antibody-4d8-western-blot.jpg)
Western blot - Anti-CTAG1B antibody [4D8] (ab139339)All lanes : Anti-CTAG1B antibody [4D8] (ab139339) at 1/2000 dilution
Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY CTAG1B cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 18 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC207790) -
![Immunocytochemistry/ Immunofluorescence - Anti-CTAG1B antibody [4D8] (ab139339)](https://www.abcam.com/ps/products/139/ab139339/Images/ab139339-154660-anti-ctag1b-antibody-4d8-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CTAG1B antibody [4D8] (ab139339)immunofluorescent analysis of COS7 cells transiently transfected with pCMV6-ENTRY CTAG1B, labelling CTAG1B with ab139339 at 1/100 dilution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab139339 has not yet been referenced specifically in any publications.
