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    ctag1b-antibody-4d8-ab139339.pdf

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Anti-CTAG1B antibody [4D8] (ab139339)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [4D8] (ab139339)
  • Western blot - Anti-CTAG1B antibody [4D8] (ab139339)
  • Immunocytochemistry/ Immunofluorescence - Anti-CTAG1B antibody [4D8] (ab139339)

Key features and details

  • Mouse monoclonal [4D8] to CTAG1B
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-CTAG1B antibody [4D8]
    See all CTAG1B primary antibodies
  • Description

    Mouse monoclonal [4D8] to CTAG1B
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length Human CTAG1B (amino acids 1-180) produced in HEK293T cells.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • HEK293T cell lysate transfected with pCMV6-ENTRY CTAG1B cDNA; COS7 cells transiently transfected with pCMV6-ENTRY CTAG1B cDNA; Human pancreas tissue.
  • General notes

    Dilute in PBS (pH7.3) before use.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, 50% Glycerol, 48% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    ab139339 is purified from TCS by affinity chromatography.
  • Clonality

    Monoclonal
  • Clone number

    4D8
  • Isotype

    IgG1
  • Research areas

    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Cancer
    • Tumor biomarkers
    • Tumor antigens

Associated products

  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

Applications

Our Abpromise guarantee covers the use of ab139339 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 18 kDa.
IHC-P 1/150. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100.

Target

  • Tissue specificity

    Expressed in testis and ovary and in a wide variety of cancers. Detected in uterine myometrium. Expressed from 18 weeks until birth in human fetal testis. In the adult testis, is strongly expressed in spermatogonia and in primary spermatocytes, but not in post-meiotic cells or in testicular somatic cells (at protein level).
  • Sequence similarities

    Belongs to the CTAG family.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession P78358 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1485 Human
    • Entrez Gene: 246100 Human
    • Omim: 300156 Human
    • SwissProt: P78358 Human
    • Unigene: 534310 Human
    • Unigene: 729551 Human
    • Alternative names

      • CTAG1A antibody
      • CTAG1B antibody
      • Autoimmunogenic cancer/testis antigen NY ESO 1 antibody
      • Autoimmunogenic cancer/testis antigen NY-ESO-1 antibody
      • Cancer antigen 3 antibody
      • Cancer/testis antigen 1 antibody
      • Cancer/testis antigen 1B antibody
      • Cancer/testis antigen 6.1 antibody
      • CT6.1 antibody
      • CTAG 1 antibody
      • CTAG 1B antibody
      • CTAG antibody
      • CTAG1 antibody
      • CTAG1B antibody
      • CTG1B_HUMAN antibody
      • ESO 1 antibody
      • ESO1 antibody
      • L antigen family member 2 antibody
      • LAGE 2 antibody
      • LAGE 2 protein antibody
      • LAGE 2B antibody
      • LAGE-2 antibody
      • LAGE2 antibody
      • LAGE2 protein antibody
      • LAGE2A antibody
      • LAGE2B antibody
      • New York esophageal squamous cell carcinoma 1 antibody
      • NY ESO 1 antibody
      • NYESO 1 antibody
      • NYESO1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [4D8] (ab139339)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [4D8] (ab139339)
      Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labelling CTAG1B with ab139339 at 1/150 dilution.
    • Western blot - Anti-CTAG1B antibody [4D8] (ab139339)
      Western blot - Anti-CTAG1B antibody [4D8] (ab139339)
      All lanes : Anti-CTAG1B antibody [4D8] (ab139339) at 1/2000 dilution

      Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
      Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY CTAG1B cDNA

      Lysates/proteins at 5 µg per lane.

      Predicted band size: 18 kDa



      HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC207790)
    • Immunocytochemistry/ Immunofluorescence - Anti-CTAG1B antibody [4D8] (ab139339)
      Immunocytochemistry/ Immunofluorescence - Anti-CTAG1B antibody [4D8] (ab139339)
      immunofluorescent analysis of COS7 cells transiently transfected with pCMV6-ENTRY CTAG1B, labelling CTAG1B with ab139339 at 1/100 dilution.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab139339? Please let us know so that we can cite the reference in this datasheet.

    ab139339 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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