Anti-CTAG1B antibody [4D8] (ab139339)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-CTAG1B antibody [4D8]
See all CTAG1B primary antibodies -
Description
Mouse monoclonal [4D8] to CTAG1B -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length Human CTAG1B (amino acids 1-180) produced in HEK293T cells.
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Positive control
- HEK293T cell lysate transfected with pCMV6-ENTRY CTAG1B cDNA; COS7 cells transiently transfected with pCMV6-ENTRY CTAG1B cDNA; Human pancreas tissue.
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General notes
Dilute in PBS (pH7.3) before use.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 50% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
ab139339 is purified from TCS by affinity chromatography. -
Clonality
Monoclonal -
Clone number
4D8 -
Isotype
IgG1 -
Research areas
Associated products
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab139339 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000. Predicted molecular weight: 18 kDa. | |
| IHC-P | 1/150. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/100. |
Target
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Tissue specificity
Expressed in testis and ovary and in a wide variety of cancers. Detected in uterine myometrium. Expressed from 18 weeks until birth in human fetal testis. In the adult testis, is strongly expressed in spermatogonia and in primary spermatocytes, but not in post-meiotic cells or in testicular somatic cells (at protein level). -
Sequence similarities
Belongs to the CTAG family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 1485 Human
- Entrez Gene: 246100 Human
- Omim: 300156 Human
- SwissProt: P78358 Human
- Unigene: 534310 Human
- Unigene: 729551 Human
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Alternative names
- CTAG1A antibody
- CTAG1B antibody
- Autoimmunogenic cancer/testis antigen NY ESO 1 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [4D8] (ab139339)](https://www.abcam.com/ps/products/139/ab139339/Images/ab139339-154659-anti-ctag1b-antibody-4d8-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [4D8] (ab139339)Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labelling CTAG1B with ab139339 at 1/150 dilution. -
![Western blot - Anti-CTAG1B antibody [4D8] (ab139339)](https://www.abcam.com/ps/products/139/ab139339/Images/ab139339-154661-anti-ctag1b-antibody-4d8-western-blot.jpg)
Western blot - Anti-CTAG1B antibody [4D8] (ab139339)All lanes : Anti-CTAG1B antibody [4D8] (ab139339) at 1/2000 dilution
Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY CTAG1B cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 18 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC207790) -
![Immunocytochemistry/ Immunofluorescence - Anti-CTAG1B antibody [4D8] (ab139339)](https://www.abcam.com/ps/products/139/ab139339/Images/ab139339-154660-anti-ctag1b-antibody-4d8-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CTAG1B antibody [4D8] (ab139339)immunofluorescent analysis of COS7 cells transiently transfected with pCMV6-ENTRY CTAG1B, labelling CTAG1B with ab139339 at 1/100 dilution.
Datasheets and documents
References
ab139339 has not yet been referenced specifically in any publications.
