Recombinant
RabMAb

Recombinant Anti-CTAG1B antibody [EPR13780] (ab183740)

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Overview

  • Product name

    Anti-CTAG1B antibody [EPR13780]
    See all CTAG1B primary antibodies

  • Description

    Rabbit monoclonal [EPR13780] to CTAG1B

  • Host species

    Rabbit

  • Specificity

    The immunogen used for this product shares 85% homology with CTAG2/LAGE1. Cross-reactivity with this protein has not been confirmed experimentally.

  • Tested applications

    Suitable for: IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein corresponding to Human CTAG1B aa 1 to the C-terminus.
    Database link: P78358

  • Positive control

    • Human melanoma and testis tissue.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR13780

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183740 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/2000 - 1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Tissue specificity

    Expressed in testis and ovary and in a wide variety of cancers. Detected in uterine myometrium. Expressed from 18 weeks until birth in human fetal testis. In the adult testis, is strongly expressed in spermatogonia and in primary spermatocytes, but not in post-meiotic cells or in testicular somatic cells (at protein level).

  • Sequence similarities

    Belongs to the CTAG family.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    • Alternative names

      • CTAG1A antibody
      • CTAG1B antibody
      • Autoimmunogenic cancer/testis antigen NY ESO 1 antibody
      • Autoimmunogenic cancer/testis antigen NY-ESO-1 antibody
      • Cancer antigen 3 antibody
      • Cancer/testis antigen 1 antibody
      • Cancer/testis antigen 1B antibody
      • Cancer/testis antigen 6.1 antibody
      • CT6.1 antibody
      • CTAG 1 antibody
      • CTAG 1B antibody
      • CTAG antibody
      • CTAG1 antibody
      • CTAG1B antibody
      • CTG1B_HUMAN antibody
      • ESO 1 antibody
      • ESO1 antibody
      • L antigen family member 2 antibody
      • LAGE 2 antibody
      • LAGE 2 protein antibody
      • LAGE 2B antibody
      • LAGE-2 antibody
      • LAGE2 antibody
      • LAGE2 protein antibody
      • LAGE2A antibody
      • LAGE2B antibody
      • New York esophageal squamous cell carcinoma 1 antibody
      • NY ESO 1 antibody
      • NYESO 1 antibody
      • NYESO1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [EPR13780] (ab183740)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [EPR13780] (ab183740)

      Immunohistochemical analysis of paraffin-embedded Human melanoma tissue labeling CTAG1B with ab183740 at 1/4000 dilution. The slide is counterstained with Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [EPR13780] (ab183740)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [EPR13780] (ab183740)

      Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling CTAG1B with ab183740 at 1/4000 dilution. The slide is counterstained with Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    References

    ab183740 has not yet been referenced specifically in any publications.

    Publishing research using ab183740? Please let us know so that we can cite the reference in this datasheet.

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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CTAG1B antibody [EPR13780]

     Average
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (melanoma xenograft)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
    Permeabilization
    No
    Specification
    melanoma xenograft
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: RT°C
    Fixative
    10% NBF
    Read More

    Abcam user community

    Verified customer

    Submitted May 07 2018

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