Recombinant Anti-CTAG1B antibody [SP349] (ab223498)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP349] to CTAG1B
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CTAG1B antibody [SP349]
See all CTAG1B primary antibodies -
Description
Rabbit monoclonal [SP349] to CTAG1B -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human CTAG1B.
Database link: P78358 -
Positive control
- IHC-P: Human testis, and Human gastric carcinoma tissue; WB: NY-ESO-1 or LAGE-2 transfected HEK-293 cell lysates.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
SP349 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab223498 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/100.
|
|
IHC-P |
1/100.
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|
Flow Cyt |
1/100.
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Notes |
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WB
1/100. |
IHC-P
1/100. |
Flow Cyt
1/100. |
Target
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Tissue specificity
Expressed in testis and ovary and in a wide variety of cancers. Detected in uterine myometrium. Expressed from 18 weeks until birth in human fetal testis. In the adult testis, is strongly expressed in spermatogonia and in primary spermatocytes, but not in post-meiotic cells or in testicular somatic cells (at protein level). -
Sequence similarities
Belongs to the CTAG family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 1485 Human
- Entrez Gene: 246100 Human
- Omim: 300156 Human
- SwissProt: P78358 Human
- Unigene: 534310 Human
- Unigene: 729551 Human
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Alternative names
- CTAG1A antibody
- CTAG1B antibody
- Autoimmunogenic cancer/testis antigen NY ESO 1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human gastric carcinoma tissue sections labeling CTAG1B with Purified ab223498 at 1/100 dilution (3.83 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human testis tissue sections labeling CTAG1B with Purified ab223498 at 1/100 dilution (3.83 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Flow Cytometry analysis of NCI-H1299 (human lung carcinoma epithelial cell) cells labeling CTAG1B with purified ab223498 at 1:380 dilution (1.01 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue.
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Formalin-fixed, paraffin-embedded human testis tissue stained for CTAG1B using ab223498 at 1/100 dilution in immunohistochemical analysis.
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All lanes : Anti-CTAG1B antibody [SP349] (ab223498) at 1/100 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) transfected with LAGE-1 cell lysate.
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) CTAG1B isoform1a transfected cell lysate. -
Flow cytometric analysis of ab223498 staining HeLa (human epithelial cell line from cervix adenocarcinoma) cells for CTAG1B (green) at 1/100 dilution compared to negative control of rabbit IgG (blue).
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Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for CTAG1B with ab223498 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab223498 has not yet been referenced specifically in any publications.