Overview

  • Product name

    Anti-CTAG1B antibody [SP349]
    See all CTAG1B primary antibodies
  • Description

    Rabbit monoclonal [SP349] to CTAG1B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human CTAG1B.
    Database link: P78358

  • Positive control

    • IHC-P: Human testis, and Human gastric carcinoma tissue; WB: NY-ESO-1 or LAGE-1 transfected HEK-293 cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.6
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Clone number

    SP349
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab223498 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100.
IHC-P 1/100.
Flow Cyt 1/100.

Target

  • Tissue specificity

    Expressed in testis and ovary and in a wide variety of cancers. Detected in uterine myometrium. Expressed from 18 weeks until birth in human fetal testis. In the adult testis, is strongly expressed in spermatogonia and in primary spermatocytes, but not in post-meiotic cells or in testicular somatic cells (at protein level).
  • Sequence similarities

    Belongs to the CTAG family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • CTAG1A antibody
    • CTAG1B antibody
    • Autoimmunogenic cancer/testis antigen NY ESO 1 antibody
    • Autoimmunogenic cancer/testis antigen NY-ESO-1 antibody
    • Cancer antigen 3 antibody
    • Cancer/testis antigen 1 antibody
    • Cancer/testis antigen 1B antibody
    • Cancer/testis antigen 6.1 antibody
    • CT6.1 antibody
    • CTAG 1 antibody
    • CTAG 1B antibody
    • CTAG antibody
    • CTAG1 antibody
    • CTAG1B antibody
    • CTG1B_HUMAN antibody
    • ESO 1 antibody
    • ESO1 antibody
    • L antigen family member 2 antibody
    • LAGE 2 antibody
    • LAGE 2 protein antibody
    • LAGE 2B antibody
    • LAGE-2 antibody
    • LAGE2 antibody
    • LAGE2 protein antibody
    • LAGE2A antibody
    • LAGE2B antibody
    • New York esophageal squamous cell carcinoma 1 antibody
    • NY ESO 1 antibody
    • NYESO 1 antibody
    • NYESO1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human gastric carcinoma tissue sections labeling CTAG1B with Purified ab223498 at 1/100 dilution (3.83 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human testis tissue sections labeling CTAG1B with Purified ab223498 at 1/100 dilution (3.83 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Flow Cytometry analysis of NCI-H1299 (human lung carcinoma epithelial cell) cells labeling CTAG1B with purified ab223498 at 1:380 dilution (1.01 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue.
  • Formalin-fixed, paraffin-embedded human testis tissue stained for CTAG1B using ab223498 at 1/100 dilution in immunohistochemical analysis.

  • All lanes : Anti-CTAG1B antibody [SP349] (ab223498) at 1/100 dilution

    Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) transfected with LAGE-1 cell lysate.
    Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) CTAG1B isoform1a transfected cell lysate.
  • Flow cytometric analysis of ab223498 staining HeLa (human epithelial cell line from cervix adenocarcinoma) cells for CTAG1B (green) at 1/100 dilution compared to negative control of rabbit IgG (blue).

  • Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for CTAG1B with ab223498 at 1/100 dilution in immunohistochemical analysis.

References

ab223498 has not yet been referenced specifically in any publications.

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