Key features and details
- Rabbit polyclonal to CTAGE5
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CTAGE5 antibody
See all CTAGE5 primary antibodies
DescriptionRabbit polyclonal to CTAGE5
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
- WB: RT4, U-251 MG sp and A431 cell lysates. IHC-P: Human hippocampus tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224218 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 91 kDa.|
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Tissue specificityIsoform 5A is expressed only in testis (at the protein level). Other isoforms are expressed in several other normal tissues, including brain, muscle and cranial skin. Isoform 5A (at protein level) and isoform 5B are expressed in cutaneous T-cell lymphoma (CTCL) cell lines, colorectal carcinomas, breast carcinomas and melanoma. Isoform 5B, but not isoform 5A, is expressed in head and neck squamous cell carcinoma.
Involvement in diseaseNote=Autoantibodies against CTAGE5 are present in several cancer types, including benign meningioma and cutaneous T-cell lymphoma (CTCL).
Sequence similaritiesBelongs to the cTAGE family.
- Information by UniProt
- cTAGE 5A antibody
- cTAGE 5B antibody
- cTAGE 5C antibody
Paraffin-embedded human hippocampus tissue stained for CTAGE5 using ab224218 at 1/200 dilution in immunohistochemical analysis.
All lanes : Anti-CTAGE5 antibody (ab224218) at 1/100 dilution
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG sp (human brain glioma cell line) cell lysate
Lane 3 : A431 (human epidermoid carcinoma cell line) cell lysate
Developed using the ECL technique.
Predicted band size: 91 kDa
ab224218 has not yet been referenced specifically in any publications.