Product nameAnti-CtBP1 antibody [EPR6800]
See all CtBP1 primary antibodies
DescriptionRabbit monoclonal [EPR6800] to CtBP1
Tested applicationsSuitable for: Flow Cyt, WB, IP, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human CtBP1 aa 350-450. The exact sequence is proprietary.
- IHC-P: Human colon tissue. WB: SH-SY5Y, Raji, A549 and HeLa whole cell lysate (ab150035). ICC/IF: HeLa cells. Flow Cyt: Raji cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 1.50 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab129181 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).|
|IP||1/10 - 1/100.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/250.|
FunctionInvolved in controlling the equilibrium between tubular and stacked structures in the Golgi complex. Functions in brown adipose tissue (BAT) differentiation. Corepressor targeting diverse transcription regulators such as GLIS2. Has dehydrogenase activity.
Sequence similaritiesBelongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.
modificationsThe level of phosphorylation appears to be regulated during the cell cycle. Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation by HIPK2 on Ser-422 induces proteasomal degradation.
ADP-ribosylated; when cells are exposed to brefeldin A.
Sumoylation on Lys-428 is promoted by the E3 SUMO-protein ligase CBX4.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- BARS antibody
- brefeldin A- ribosylated substrate antibody
- C terminal binding protein 1 antibody
All lanes : Anti-CtBP1 antibody [EPR6800] (ab129181) at 1/1000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : Raji cell lysate
Lane 3 : A549 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP-conjugated goat anti-rabbit IgG
Predicted band size: 48 kDa
Observed band size: 48 kDa
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling CtBP1 with ab129181 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
Control: PBS only.
Nuclear counter stain: DAPI.
ab129181, at 1/100 dilution, staining CtBP1 in formalin-fixed, paraffin-embedded human colon tissue by immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow cytometry analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells labeling CtBP1 (red) with ab129181 at a 1/2000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- Zhang X et al. The CtBP1-HDAC1/2-IRF1 transcriptional complex represses the expression of the long noncoding RNA GAS5 in human osteosarcoma cells. Int J Biol Sci 15:1460-1471 (2019). Read more (PubMed: 31337976) »
- Gay G et al. Toxoplasma gondii TgIST co-opts host chromatin repressors dampening STAT1-dependent gene regulation and IFN-?-mediated host defenses. J Exp Med 213:1779-98 (2016). Read more (PubMed: 27503074) »