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RecombinantRabMAb

Recombinant Anti-CtBP1 antibody [EPR6800] - BSA and Azide free (ab248335)

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Western blot - Anti-CtBP1 antibody [EPR6800] - BSA and Azide free (ab248335)
  • Immunocytochemistry - Anti-CtBP1 antibody [EPR6800] - BSA and Azide free (ab248335)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CtBP1 antibody [EPR6800] - BSA and Azide free (ab248335)
  • Flow Cytometry (Intracellular) - Anti-CtBP1 antibody [EPR6800] - BSA and Azide free (ab248335)
  • OI-RD Scanning - Anti-CtBP1 antibody [EPR6800] - BSA and Azide free (ab248335)
  • Anti-CtBP1 antibody [EPR6800] - BSA and Azide free (ab248335)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6800] to CtBP1 - BSA and Azide free
  • Suitable for: ICC, IHC-P, WB, Flow Cyt (Intra)
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

HRP Unconjugated

Overview

  • Product name

    Anti-CtBP1 antibody [EPR6800] - BSA and Azide free
    See all CtBP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR6800] to CtBP1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IHC-P, WB, Flow Cyt (Intra)more details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248335 is the carrier-free version of ab129181.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab248335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
Flow Cyt (Intra)
Use at an assay dependent concentration.
Notes
ICC
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
Flow Cyt (Intra)
Use at an assay dependent concentration.

Target

  • Function

    Involved in controlling the equilibrium between tubular and stacked structures in the Golgi complex. Functions in brown adipose tissue (BAT) differentiation. Corepressor targeting diverse transcription regulators such as GLIS2. Has dehydrogenase activity.

  • Sequence similarities

    Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.

  • Post-translational
    modifications

    The level of phosphorylation appears to be regulated during the cell cycle. Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation by HIPK2 on Ser-422 induces proteasomal degradation.
    ADP-ribosylated; when cells are exposed to brefeldin A.
    Sumoylation on Lys-428 is promoted by the E3 SUMO-protein ligase CBX4.

  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • BARS antibody
    • brefeldin A- ribosylated substrate antibody
    • C terminal binding protein 1 antibody
    • C-terminal-binding protein 1 antibody
    • CTBP antibody
    • CtBP1 antibody
    • CTBP1_HUMAN antibody
    • MGC104684 antibody
    see all

Images

  • Western blot - Anti-CtBP1 antibody [EPR6800] - BSA and Azide free (ab248335)
    Western blot - Anti-CtBP1 antibody [EPR6800] - BSA and Azide free (ab248335)
    All lanes : Anti-CtBP1 antibody [EPR6800] (ab129181) at 1/1000 dilution

    Lane 1 : SH-SY5Y cell lysate
    Lane 2 : Raji cell lysate
    Lane 3 : A549 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG

    Predicted band size: 48 kDa
    Observed band size: 48 kDa



    This data was developed using ab129181, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-CtBP1 antibody [EPR6800] - BSA and Azide free (ab248335)
    Immunocytochemistry - Anti-CtBP1 antibody [EPR6800] - BSA and Azide free (ab248335)
    This data was developed using ab129181, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling CtBP1 with ab129181 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
    Control: PBS only.
    Nuclear counter stain: DAPI.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CtBP1 antibody [EPR6800] - BSA and Azide free (ab248335)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CtBP1 antibody [EPR6800] - BSA and Azide free (ab248335)
    This data was developed using ab129181, the same antibody clone in a different buffer formulation.ab129181, at 1/100 dilution, staining CtBP1 in formalin-fixed, paraffin-embedded human colon tissue by immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Flow Cytometry (Intracellular) - Anti-CtBP1 antibody [EPR6800] - BSA and Azide free (ab248335)
    Flow Cytometry (Intracellular) - Anti-CtBP1 antibody [EPR6800] - BSA and Azide free (ab248335)

    This data was developed using ab129181, the same antibody clone in a different buffer formulation. 

    Intracellular Flow Cytometry analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells labeling CtBP1 (red) with ab129181 at a 1/2000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue). 

     

     

  • OI-RD Scanning - Anti-CtBP1 antibody [EPR6800] - BSA and Azide free (ab248335)
    OI-RD Scanning - Anti-CtBP1 antibody [EPR6800] - BSA and Azide free (ab248335)
    This data was developed using ab129181, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-CtBP1 antibody [EPR6800] - BSA and Azide free (ab248335)
    Anti-CtBP1 antibody [EPR6800] - BSA and Azide free (ab248335)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab248335? Please let us know so that we can cite the reference in this datasheet.

ab248335 has not yet been referenced specifically in any publications.

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