For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    ctbp2-antibody-ab151766.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Microbiology Organism Virus DNA Virus double stranded DNA Virus Adenovirus
Share by email

Anti-CTBP2 antibody (ab151766)

  • Datasheet
  • SDS
Reviews (1) Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-CTBP2 antibody (ab151766)
  • Immunocytochemistry/ Immunofluorescence - Anti-CTBP2 antibody (ab151766)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTBP2 antibody (ab151766)

Key features and details

  • Rabbit polyclonal to CTBP2
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human CTBP2 protein (ab114954)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-CTBP2 antibody
    See all CTBP2 primary antibodies
  • Description

    Rabbit polyclonal to CTBP2
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-172 of Human CTBP2 (UniProt: P56545).

  • Positive control

    • HepG2 and HCT116 whole cell lysates, HeLa cells, Huh7 xenograft

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Microbiology
    • Organism
    • Virus
    • DNA Virus
    • double stranded DNA Virus
    • Adenovirus
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Co-factors

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human CTBP2 protein (ab114954)
  • Related Products

    • Recombinant Human CTBP2 protein (ab114954)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab151766 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
WB
Human
All applications
Mouse
Xenopus laevis
Zebrafish
Xenopus tropicalis
Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 49 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) is also possible.
ICC/IF
1/100 - 1/1000.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 49 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) is also possible.
ICC/IF
1/100 - 1/1000.

Target

  • Function

    Corepressor targeting diverse transcription regulators. Functions in brown adipose tissue (BAT) differentiation.
    Isoform 2 probably acts as a scaffold for specialized synapses.
  • Tissue specificity

    Ubiquitous. Highest levels in heart, skeletal muscle, and pancreas.
  • Sequence similarities

    Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.
  • Post-translational
    modifications

    Isoform 2 is phosphorylated upon DNA damage, probably by ATM or ATR at Thr-179; Ser-181 and Ser-185. Phosphorylation by HIPK2 on Ser-428 induces proteasomal degradation.
  • Cellular localization

    Nucleus. Cell junction > synapse.
  • Target information above from: UniProt accession P56545 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1488 Human
    • Entrez Gene: 13017 Mouse
    • Entrez Gene: 398147 Xenopus laevis
    • Omim: 602619 Human
    • SwissProt: P56545 Human
    • SwissProt: P56546 Mouse
    • SwissProt: Q9W758 Xenopus laevis
    • SwissProt: Q9DEG6 Zebrafish
    • Unigene: 501345 Human
    • Unigene: 246240 Mouse
    • Unigene: 389984 Mouse
    • Unigene: 41670 Xenopus laevis
    see all
  • Alternative names

    • C terminal binding protein 2 antibody
    • C-terminal-binding protein 2 antibody
    • CtBP2 antibody
    • CTBP2_HUMAN antibody
    • ribeye antibody
    see all

Images

  • Western blot - Anti-CTBP2 antibody (ab151766)
    Western blot - Anti-CTBP2 antibody (ab151766)
    All lanes : Anti-CTBP2 antibody (ab151766) at 1/1000 dilution

    Lane 1 : HepG2 whole cell
    lysate
    Lane 2 : HCT116 whole cell
    lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 49 kDa



    10% SDS PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-CTBP2 antibody (ab151766)
    Immunocytochemistry/ Immunofluorescence - Anti-CTBP2 antibody (ab151766)
    Immunofluorescent analysis of methanol-fixed HeLa cells labeling CTBP2 with ab151766 at 1/500. Lower panel co-stained with Hoechst 33342.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTBP2 antibody (ab151766)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTBP2 antibody (ab151766)
    Immunohistochemical analysis of paraffin embedded Huh7 xenograft labeling CTBP2 with ab151766 antibody at 1/500.

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab151766? Please let us know so that we can cite the reference in this datasheet.

    ab151766 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-CTBP2 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Xenopus laevis Tissue sections (Retina)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10 mM Sodium citrate, 0.05% Tween 20, pH 6.0
    Permeabilization
    Yes - Triton X-100 0.1%
    Specification
    Retina
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More

    Brittany Carr

    Verified customer

    Submitted Jan 21 2021

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.