Anti-CTBP2 antibody (ab151766)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-CTBP2 antibody
See all CTBP2 primary antibodies -
Description
Rabbit polyclonal to CTBP2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Xenopus laevis, Zebrafish, Xenopus tropicalis
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-172 of Human CTBP2 (UniProt: P56545).
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Positive control
- HepG2 and HCT116 whole cell lysates, HeLa cells, Huh7 xenograft
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab151766 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 49 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) is also possible. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Corepressor targeting diverse transcription regulators. Functions in brown adipose tissue (BAT) differentiation.
Isoform 2 probably acts as a scaffold for specialized synapses. -
Tissue specificity
Ubiquitous. Highest levels in heart, skeletal muscle, and pancreas. -
Sequence similarities
Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. -
Post-translational
modificationsIsoform 2 is phosphorylated upon DNA damage, probably by ATM or ATR at Thr-179; Ser-181 and Ser-185. Phosphorylation by HIPK2 on Ser-428 induces proteasomal degradation. -
Cellular localization
Nucleus. Cell junction > synapse. - Information by UniProt
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Database links
- Entrez Gene: 1488 Human
- Entrez Gene: 13017 Mouse
- Entrez Gene: 398147 Xenopus laevis
- Omim: 602619 Human
- SwissProt: P56545 Human
- SwissProt: P56546 Mouse
- SwissProt: Q9W758 Xenopus laevis
- SwissProt: Q9DEG6 Zebrafish
see all -
Alternative names
- C terminal binding protein 2 antibody
- C-terminal-binding protein 2 antibody
- CtBP2 antibody
see all
Images
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Western blot - Anti-CTBP2 antibody (ab151766)All lanes : Anti-CTBP2 antibody (ab151766) at 1/1000 dilution
Lane 1 : HepG2 whole cell
lysate
Lane 2 : HCT116 whole cell
lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 49 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-CTBP2 antibody (ab151766)Immunofluorescent analysis of methanol-fixed HeLa cells labeling CTBP2 with ab151766 at 1/500. Lower panel co-stained with Hoechst 33342. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTBP2 antibody (ab151766)Immunohistochemical analysis of paraffin embedded Huh7 xenograft labeling CTBP2 with ab151766 antibody at 1/500.
Protocols
References
ab151766 has not yet been referenced specifically in any publications.
