Anti-CTBP2 antibody (ab151766)
Key features and details
- Rabbit polyclonal to CTBP2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CTBP2 antibody
See all CTBP2 primary antibodies -
Description
Rabbit polyclonal to CTBP2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
-
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-172 of Human CTBP2 (UniProt: P56545).
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Positive control
- HepG2 and HCT116 whole cell lysates, HeLa cells, Huh7 xenograft
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151766 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| IHC-P |
Human
|
| WB |
Human
|
| All applications |
Mouse
Xenopus laevis
Zebrafish
Xenopus tropicalis
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 49 kDa.
|
|
| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) is also possible.
|
|
| ICC/IF |
1/100 - 1/1000.
|
| Notes |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 49 kDa. |
|
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) is also possible. |
|
ICC/IF
1/100 - 1/1000. |
Target
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Function
Corepressor targeting diverse transcription regulators. Functions in brown adipose tissue (BAT) differentiation.
Isoform 2 probably acts as a scaffold for specialized synapses. -
Tissue specificity
Ubiquitous. Highest levels in heart, skeletal muscle, and pancreas. -
Sequence similarities
Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. -
Post-translational
modificationsIsoform 2 is phosphorylated upon DNA damage, probably by ATM or ATR at Thr-179; Ser-181 and Ser-185. Phosphorylation by HIPK2 on Ser-428 induces proteasomal degradation. -
Cellular localization
Nucleus. Cell junction > synapse. - Information by UniProt
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Database links
- Entrez Gene: 1488 Human
- Entrez Gene: 13017 Mouse
- Entrez Gene: 398147 Xenopus laevis
- Omim: 602619 Human
- SwissProt: P56545 Human
- SwissProt: P56546 Mouse
- SwissProt: Q9W758 Xenopus laevis
- SwissProt: Q9DEG6 Zebrafish
see all -
Alternative names
- C terminal binding protein 2 antibody
- C-terminal-binding protein 2 antibody
- CtBP2 antibody
see all
Images
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Western blot - Anti-CTBP2 antibody (ab151766)All lanes : Anti-CTBP2 antibody (ab151766) at 1/1000 dilution
Lane 1 : HepG2 whole cell
lysate
Lane 2 : HCT116 whole cell
lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 49 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-CTBP2 antibody (ab151766)Immunofluorescent analysis of methanol-fixed HeLa cells labeling CTBP2 with ab151766 at 1/500. Lower panel co-stained with Hoechst 33342. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTBP2 antibody (ab151766)Immunohistochemical analysis of paraffin embedded Huh7 xenograft labeling CTBP2 with ab151766 antibody at 1/500.
Protocols
References (0)
ab151766 has not yet been referenced specifically in any publications.
