Overview

  • Product name

  • Description

    Rabbit polyclonal to CTBP2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat, Cow, Monkey, Zebrafish
  • Immunogen

    Recombinant full length protein, corresponding to a region within amino acids 737-927 of Human CTBP2 (NP_073713).

  • Positive control

    • Molt-4 whole cell lysate (WB) and HeLa cell (IF)

Properties

Applications

Our Abpromise guarantee covers the use of ab96107 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 106 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Function

    Corepressor targeting diverse transcription regulators. Functions in brown adipose tissue (BAT) differentiation.
    Isoform 2 probably acts as a scaffold for specialized synapses.
  • Tissue specificity

    Ubiquitous. Highest levels in heart, skeletal muscle, and pancreas.
  • Sequence similarities

    Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.
  • Post-translational
    modifications

    Isoform 2 is phosphorylated upon DNA damage, probably by ATM or ATR at Thr-179; Ser-181 and Ser-185. Phosphorylation by HIPK2 on Ser-428 induces proteasomal degradation.
  • Cellular localization

    Nucleus. Cell junction > synapse.
  • Information by UniProt
  • Database links

  • Alternative names

    • C terminal binding protein 2 antibody
    • C-terminal-binding protein 2 antibody
    • CtBP2 antibody
    • CTBP2_HUMAN antibody
    • ribeye antibody
    see all

Images

  • Anti-CTBP2 antibody (ab96107) at 1/500 dilution + Molt-4 whole cell lysate at 30 µg

    Predicted band size: 106 kDa



    7.5% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab96107 at 1/100 dilution.
  • Anti-CTBP2 antibody (ab96107) at 1/200 dilution + zebrafish embryo tissue lysate at 40 µg

    Secondary
    HRP-conjugated goat anti-rabbit polyclonal IgG at 1/4000 dilution

    Predicted band size: 106 kDa
    Observed band size: 55-60,85-90 kDa
    why is the actual band size different from the predicted?



    Blocked with 5% milk for 1 hour at 25°C

    See Abreview

References

ab96107 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Loading amount
50 µg
Specification
HEK293
Gel Running Conditions
Reduced Denaturing (4°12% Bis-Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Evgenia Salta

Verified customer

Submitted Jul 17 2012

Application
Western blot
Sample
Zebrafish Tissue lysate - whole (Whole-mount, Larvae, 3 dpf)
Loading amount
40 µg
Specification
Whole-mount, Larvae, 3 dpf
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Evgenia Salta

Verified customer

Submitted Jul 11 2012

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