Key features and details
- Rabbit polyclonal to CTBP2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CTBP2 antibody
See all CTBP2 primary antibodies
DescriptionRabbit polyclonal to CTBP2
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Rat, Cow, Monkey, Zebrafish
Recombinant full length protein, corresponding to a region within amino acids 737-927 of Human CTBP2 (NP_073713).
- Molt-4 whole cell lysate (WB) and HeLa cell (IF)
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab96107 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 106 kDa.|
|ICC/IF||1/100 - 1/200.|
FunctionCorepressor targeting diverse transcription regulators. Functions in brown adipose tissue (BAT) differentiation.
Isoform 2 probably acts as a scaffold for specialized synapses.
Tissue specificityUbiquitous. Highest levels in heart, skeletal muscle, and pancreas.
Sequence similaritiesBelongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.
modificationsIsoform 2 is phosphorylated upon DNA damage, probably by ATM or ATR at Thr-179; Ser-181 and Ser-185. Phosphorylation by HIPK2 on Ser-428 induces proteasomal degradation.
Cellular localizationNucleus. Cell junction > synapse.
- Information by UniProt
- C terminal binding protein 2 antibody
- C-terminal-binding protein 2 antibody
- CtBP2 antibody
Anti-CTBP2 antibody (ab96107) at 1/500 dilution + Molt-4 whole cell lysate at 30 µg
Predicted band size: 106 kDa
7.5% SDS PAGE
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab96107 at 1/100 dilution.
Anti-CTBP2 antibody (ab96107) at 1/200 dilution + zebrafish embryo tissue lysate at 40 µg
HRP-conjugated goat anti-rabbit polyclonal IgG at 1/4000 dilution
Predicted band size: 106 kDa
Observed band size: 55-60,85-90 kDa why is the actual band size different from the predicted?
Blocked with 5% milk for 1 hour at 25°C
ab96107 has not yet been referenced specifically in any publications.