Product nameAnti-CTBP2 antibody - C-terminal
See all CTBP2 primary antibodies
DescriptionRabbit polyclonal to CTBP2 - C-terminal
Tested applicationsSuitable for: ICC/IF, IHC-Fr, WB, IHC-P, ICCmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow
- Rat brain and spleen tissue lysates; HeLa, 293T, COLO320, U87, SW620 cell lysates; Human mammary cancer tissue; rat intestine tissue; HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Sodium phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab190933 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.5 - 1 µg/ml.|
|IHC-Fr||Use a concentration of 0.5 - 1 µg/ml.|
|WB||Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 49 kDa.|
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC||Use a concentration of 0.5 - 1 µg/ml.|
FunctionCorepressor targeting diverse transcription regulators. Functions in brown adipose tissue (BAT) differentiation.
Isoform 2 probably acts as a scaffold for specialized synapses.
Tissue specificityUbiquitous. Highest levels in heart, skeletal muscle, and pancreas.
Sequence similaritiesBelongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.
modificationsIsoform 2 is phosphorylated upon DNA damage, probably by ATM or ATR at Thr-179; Ser-181 and Ser-185. Phosphorylation by HIPK2 on Ser-428 induces proteasomal degradation.
Cellular localizationNucleus. Cell junction > synapse.
- Information by UniProt
- C terminal binding protein 2 antibody
- C-terminal-binding protein 2 antibody
- CtBP2 antibody
All lanes : Anti-CTBP2 antibody - C-terminal (ab190933) at 0.5 µg/ml
Lane 1 : Rat brain tissue lysate
Lane 2 : Rat spleen tissue lysate
Lane 3 : HeLa cell lysate
Lane 4 : 293T cell lysate
Lane 5 : COLO320 cell lysate
Lane 6 : U87 cell lysate
Lane 7 : SW620 cell lysate
Predicted band size: 49 kDa
Immunocytochemical analysis of HeLa cells labeling CTBP2 using ab190933 at 1 μg/ml.
Immunohistochemical analysis of frozen rat intestine tissue labeling CTBP2 using ab190933 at 1 μg/ml.
Immunohistochemical analysis of paraffin embedded Human mammary cancer tissue labeling CTBP2 using ab190933 at 1 μg/ml.
ab190933 has not yet been referenced specifically in any publications.