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RecombinantRabMAb

Recombinant Anti-CTBP2 antibody [EPR7611(B)] (ab128871)

  • Datasheet
  • SDS
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Western blot - Anti-CTBP2 antibody [EPR7611(B)] (ab128871)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTBP2 antibody [EPR7611(B)] (ab128871)
  • Immunocytochemistry/ Immunofluorescence - Anti-CTBP2 antibody [EPR7611(B)] (ab128871)
  • Anti-CTBP2 antibody [EPR7611(B)] (ab128871)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7611(B)] to CTBP2
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

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Overview

  • Product name

    Anti-CTBP2 antibody [EPR7611(B)]
    See all CTBP2 primary antibodies
  • Description

    Rabbit monoclonal [EPR7611(B)] to CTBP2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human CTBP2 aa 400-500 (C terminal). The exact sequence is proprietary.

  • Positive control

    • HeLa, SKBR-3 and Saos 2 cell lysates; Human thyroid carcinoma tissue; SKBR-3 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR7611(B)
  • Isotype

    IgG
  • Research areas

    • Microbiology
    • Organism
    • Virus
    • DNA Virus
    • double stranded DNA Virus
    • Adenovirus
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Co-factors

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-CTBP2 antibody [EPR7611(B)] (ab204662)
    • Alexa Fluor® 647 Anti-CTBP2 antibody [EPR7611(B)] (ab204663)
    • Anti-CTBP2 antibody [EPR7611(B)] - BSA and Azide free (ab248197)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa nuclear extract lysate (ab14655)
    • Saos-2 nuclear extract lysate (ab14891)
  • Recombinant Protein

    • Recombinant Human CTBP2 protein (ab114954)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab128871 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/10000 - 1/50000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
Notes
WB
1/10000 - 1/50000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Corepressor targeting diverse transcription regulators. Functions in brown adipose tissue (BAT) differentiation.
      Isoform 2 probably acts as a scaffold for specialized synapses.
    • Tissue specificity

      Ubiquitous. Highest levels in heart, skeletal muscle, and pancreas.
    • Sequence similarities

      Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.
    • Post-translational
      modifications

      Isoform 2 is phosphorylated upon DNA damage, probably by ATM or ATR at Thr-179; Ser-181 and Ser-185. Phosphorylation by HIPK2 on Ser-428 induces proteasomal degradation.
    • Cellular localization

      Nucleus. Cell junction > synapse.
    • Target information above from: UniProt accession P56545 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 1488 Human
      • Entrez Gene: 13017 Mouse
      • Entrez Gene: 81717 Rat
      • Omim: 602619 Human
      • SwissProt: P56545 Human
      • SwissProt: P56546 Mouse
      • SwissProt: Q9EQH5 Rat
      • Unigene: 501345 Human
      • Unigene: 246240 Mouse
      • Unigene: 389984 Mouse
      • Unigene: 138124 Rat
      see all
    • Alternative names

      • C terminal binding protein 2 antibody
      • C-terminal-binding protein 2 antibody
      • CtBP2 antibody
      • CTBP2_HUMAN antibody
      • ribeye antibody
      see all

    Images

    • Western blot - Anti-CTBP2 antibody [EPR7611(B)] (ab128871)
      Western blot - Anti-CTBP2 antibody [EPR7611(B)] (ab128871)
      All lanes : Anti-CTBP2 antibody [EPR7611(B)] (ab128871) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : SKBR-3 cell lysate
      Lane 3 : Saos 2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 49 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTBP2 antibody [EPR7611(B)] (ab128871)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTBP2 antibody [EPR7611(B)] (ab128871)

      ab128871 at 1/250 dilution staining CTBP2 in Paraffin-embedded Human thyroid carcinoma tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-CTBP2 antibody [EPR7611(B)] (ab128871)
      Immunocytochemistry/ Immunofluorescence - Anti-CTBP2 antibody [EPR7611(B)] (ab128871)
      ab128871 at 1/100 dilution staining CTBP2 in SKBR-3 cells by Immunofluorescence.
    • Anti-CTBP2 antibody [EPR7611(B)] (ab128871)
      Anti-CTBP2 antibody [EPR7611(B)] (ab128871)

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (7)

    Publishing research using ab128871? Please let us know so that we can cite the reference in this datasheet.

    ab128871 has been referenced in 7 publications.

    • Zhou P  et al. Transforming growth factor beta (TGF-ß) is activated by the CtBP2-p300-AP1 transcriptional complex in chronic renal failure. Int J Biol Sci 16:204-215 (2020). PubMed: 31929749
    • Li S  et al. FGF22 promotes generation of ribbon synapses through downregulating MEF2D. Aging (Albany NY) 12:6456-6466 (2020). PubMed: 32271716
    • Wang H  et al. Decreased levels of superoxide dismutase in inner pillar cells contribute to ribbon synapse impairment in presbycusis. Am J Transl Res 11:2403-2412 (2019). PubMed: 31105846
    • Crespo-Garcia S  et al. Individual and temporal variability of the retina after chronic bilateral common carotid artery occlusion (BCCAO). PLoS One 13:e0193961 (2018). PubMed: 29547662
    • Dai F  et al. CtBP2 overexpression promotes tumor cell proliferation and invasion in gastric cancer and is associated with poor prognosis. Oncotarget 8:28736-28749 (2017). IHC . PubMed: 28404932
    • Wang Y  et al. Expression and prognostic significance of CTBP2 in human gliomas. Oncol Lett 12:2429-2434 (2016). WB, IHC-P ; Human . PubMed: 27698809
    • Karaca E  et al. Oxygen-dependent acetylation and dimerization of the corepressor CtBP2 in neural stem cells. Exp Cell Res 332:128-35 (2015). PubMed: 25447313

    Customer reviews and Q&As

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