Overview

  • Product name
    Anti-CTBP2 antibody [EPR7611(B)]
    See all CTBP2 primary antibodies
  • Description
    Rabbit monoclonal [EPR7611(B)] to CTBP2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CTBP2 aa 400-500 (C terminal). The exact sequence is proprietary.

  • Positive control
    • HeLa, SKBR-3 and Saos 2 cell lysates; Human thyroid carcinoma tissue; SKBR-3 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab128871 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Corepressor targeting diverse transcription regulators. Functions in brown adipose tissue (BAT) differentiation.
      Isoform 2 probably acts as a scaffold for specialized synapses.
    • Tissue specificity
      Ubiquitous. Highest levels in heart, skeletal muscle, and pancreas.
    • Sequence similarities
      Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.
    • Post-translational
      modifications
      Isoform 2 is phosphorylated upon DNA damage, probably by ATM or ATR at Thr-179; Ser-181 and Ser-185. Phosphorylation by HIPK2 on Ser-428 induces proteasomal degradation.
    • Cellular localization
      Nucleus. Cell junction > synapse.
    • Information by UniProt
    • Database links
    • Alternative names
      • C terminal binding protein 2 antibody
      • C-terminal-binding protein 2 antibody
      • CtBP2 antibody
      • CTBP2_HUMAN antibody
      • ribeye antibody
      see all

    Images

    • All lanes : Anti-CTBP2 antibody [EPR7611(B)] (ab128871) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : SKBR-3 cell lysate
      Lane 3 : Saos 2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 49 kDa

    • ab128871 at 1/250 dilution staining CTBP2 in Paraffin-embedded Human thyroid carcinoma tissue by Immunohistochemistry.
    • ab128871 at 1/100 dilution staining CTBP2 in SKBR-3 cells by Immunofluorescence.

    References

    This product has been referenced in:
    • Crespo-Garcia S  et al. Individual and temporal variability of the retina after chronic bilateral common carotid artery occlusion (BCCAO). PLoS One 13:e0193961 (2018). Read more (PubMed: 29547662) »
    • Dai F  et al. CtBP2 overexpression promotes tumor cell proliferation and invasion in gastric cancer and is associated with poor prognosis. Oncotarget 8:28736-28749 (2017). IHC . Read more (PubMed: 28404932) »

    See all 4 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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