Recombinant Anti-CTBP2 antibody [EPR7611(B)] (ab128871)
![Western blot - Anti-CTBP2 antibody [EPR7611(B)] (ab128871)](https://www.abcam.com/ps/products/128/ab128871/Images/ab128871-142646-anti-ctbp2-antibody-epr7611b-western-blot.jpg "Western blot - Anti-CTBP2 antibody [EPR7611(B)] (ab128871)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7611(B)] to CTBP2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-CTBP2 antibody [EPR7611(B)]
See all CTBP2 primary antibodies -
Description
Rabbit monoclonal [EPR7611(B)] to CTBP2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human CTBP2 aa 400-500 (C terminal). The exact sequence is proprietary.
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Positive control
- HeLa, SKBR-3 and Saos 2 cell lysates; Human thyroid carcinoma tissue; SKBR-3 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7611(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab128871 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/10000 - 1/50000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
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| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| ICC/IF |
1/100 - 1/250.
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| Notes |
|---|
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WB
1/10000 - 1/50000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa). |
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IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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ICC/IF
1/100 - 1/250. |
Application notes
Is unsuitable for Flow Cyt or IP.
Target
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Function
Corepressor targeting diverse transcription regulators. Functions in brown adipose tissue (BAT) differentiation.
Isoform 2 probably acts as a scaffold for specialized synapses. -
Tissue specificity
Ubiquitous. Highest levels in heart, skeletal muscle, and pancreas. -
Sequence similarities
Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. -
Post-translational
modificationsIsoform 2 is phosphorylated upon DNA damage, probably by ATM or ATR at Thr-179; Ser-181 and Ser-185. Phosphorylation by HIPK2 on Ser-428 induces proteasomal degradation. -
Cellular localization
Nucleus. Cell junction > synapse. - Information by UniProt
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Database links
- Entrez Gene: 1488 Human
- Entrez Gene: 13017 Mouse
- Entrez Gene: 81717 Rat
- Omim: 602619 Human
- SwissProt: P56545 Human
- SwissProt: P56546 Mouse
- SwissProt: Q9EQH5 Rat
- Unigene: 501345 Human
see all -
Alternative names
- C terminal binding protein 2 antibody
- C-terminal-binding protein 2 antibody
- CtBP2 antibody
see all
Images
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Western blot - Anti-CTBP2 antibody [EPR7611(B)] (ab128871)All lanes : Anti-CTBP2 antibody [EPR7611(B)] (ab128871) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : SKBR-3 cell lysate
Lane 3 : Saos 2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 49 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTBP2 antibody [EPR7611(B)] (ab128871)](https://www.abcam.com/ps/products/128/ab128871/Images/ab128871-142645-anti-ctbp2-antibody-epr7611b-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTBP2 antibody [EPR7611(B)] (ab128871)ab128871 at 1/250 dilution staining CTBP2 in Paraffin-embedded Human thyroid carcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-CTBP2 antibody [EPR7611(B)] (ab128871)](https://www.abcam.com/ps/products/128/ab128871/Images/ab128871-142644-anti-ctbp2-antibody-epr7611b-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CTBP2 antibody [EPR7611(B)] (ab128871)ab128871 at 1/100 dilution staining CTBP2 in SKBR-3 cells by Immunofluorescence. -
![Anti-CTBP2 antibody [EPR7611(B)] (ab128871)](https://www.abcam.com/ps/products/128/ab128871/Images/ab128871-4-benefits-of-recombinant-antibodies.png)
Anti-CTBP2 antibody [EPR7611(B)] (ab128871)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab128871 has been referenced in 7 publications.
- Zhou P et al. Transforming growth factor beta (TGF-ß) is activated by the CtBP2-p300-AP1 transcriptional complex in chronic renal failure. Int J Biol Sci 16:204-215 (2020). PubMed: 31929749
- Li S et al. FGF22 promotes generation of ribbon synapses through downregulating MEF2D. Aging (Albany NY) 12:6456-6466 (2020). PubMed: 32271716
- Wang H et al. Decreased levels of superoxide dismutase in inner pillar cells contribute to ribbon synapse impairment in presbycusis. Am J Transl Res 11:2403-2412 (2019). PubMed: 31105846
- Crespo-Garcia S et al. Individual and temporal variability of the retina after chronic bilateral common carotid artery occlusion (BCCAO). PLoS One 13:e0193961 (2018). PubMed: 29547662
- Dai F et al. CtBP2 overexpression promotes tumor cell proliferation and invasion in gastric cancer and is associated with poor prognosis. Oncotarget 8:28736-28749 (2017). IHC . PubMed: 28404932
- Wang Y et al. Expression and prognostic significance of CTBP2 in human gliomas. Oncol Lett 12:2429-2434 (2016). WB, IHC-P ; Human . PubMed: 27698809
- Karaca E et al. Oxygen-dependent acetylation and dimerization of the corepressor CtBP2 in neural stem cells. Exp Cell Res 332:128-35 (2015). PubMed: 25447313