Anti-CTBP2 antibody [EPR7611(B)] Recombinant (ab128871)

Product name

Anti-CTBP2 antibody [EPR7611(B)]
See all CTBP2 primary antibodies
Description

Rabbit monoclonal [EPR7611(B)] to CTBP2
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide within Human CTBP2 aa 400-500 (C terminal). The exact sequence is proprietary.
Positive control
- HeLa, SKBR-3 and Saos 2 cell lysates; Human thyroid carcinoma tissue; SKBR-3 cells.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR7611(B)
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab128871 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/10000 - 1/50000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
IHC-P		1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF		1/100 - 1/250.

Application notes

Is unsuitable for Flow Cyt or IP.

Function

Corepressor targeting diverse transcription regulators. Functions in brown adipose tissue (BAT) differentiation.
Isoform 2 probably acts as a scaffold for specialized synapses.
Tissue specificity

Ubiquitous. Highest levels in heart, skeletal muscle, and pancreas.
Sequence similarities

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.
Post-translational
modifications

Isoform 2 is phosphorylated upon DNA damage, probably by ATM or ATR at Thr-179; Ser-181 and Ser-185. Phosphorylation by HIPK2 on Ser-428 induces proteasomal degradation.
Cellular localization

Nucleus. Cell junction > synapse.
Target information above from: UniProt accession P56545 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 1488 Human
- Entrez Gene: 13017 Mouse
- Entrez Gene: 81717 Rat
- Omim: 602619 Human
- SwissProt: P56545 Human
- SwissProt: P56546 Mouse
- SwissProt: Q9EQH5 Rat
- Unigene: 501345 Human
- Unigene: 246240 Mouse
- Unigene: 389984 Mouse
- Unigene: 138124 Rat
see all
Alternative names
- C terminal binding protein 2 antibody
- C-terminal-binding protein 2 antibody
- CtBP2 antibody
- CTBP2_HUMAN antibody
- ribeye antibody
see all

Western blot - Anti-CTBP2 antibody [EPR7611(B)] (ab128871)

All lanes : Anti-CTBP2 antibody [EPR7611(B)] (ab128871) at 1/10000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : SKBR-3 cell lysate
Lane 3 : Saos 2 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

Developed using the ECL technique.

Predicted band size: 49 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTBP2 antibody [EPR7611(B)] (ab128871)

ab128871 at 1/250 dilution staining CTBP2 in Paraffin-embedded Human thyroid carcinoma tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-CTBP2 antibody [EPR7611(B)] (ab128871)

ab128871 at 1/100 dilution staining CTBP2 in SKBR-3 cells by Immunofluorescence.

Crespo-Garcia S et al. Individual and temporal variability of the retina after chronic bilateral common carotid artery occlusion (BCCAO). PLoS One 13:e0193961 (2018). Read more (PubMed: 29547662) »
Dai F et al. CtBP2 overexpression promotes tumor cell proliferation and invasion in gastric cancer and is associated with poor prognosis. Oncotarget 8:28736-28749 (2017). IHC . Read more (PubMed: 28404932) »

Publishing research using ab128871? Please let us know so that we can cite the reference in this datasheet.

Anti-CTBP2 antibody [EPR7611(B)] (ab128871)