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Rabbit monoclonal [EPR7611(B)] to CTBP2
ICC/IF more details Unsuitable for:
Flow Cyt or IP
Mouse, Rat, Human
Synthetic peptide within Human CTBP2 aa 400-500 (C terminal). The exact sequence is proprietary.
HeLa, SKBR-3 and Saos 2 cell lysates; Human thyroid carcinoma tissue; SKBR-3 cells.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.01% Sodium azide Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10000 - 1/50000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
1/100 - 1/250.
Is unsuitable for Flow Cyt or IP.
Corepressor targeting diverse transcription regulators. Functions in brown adipose tissue (BAT) differentiation.
Isoform 2 probably acts as a scaffold for specialized synapses.
Ubiquitous. Highest levels in heart, skeletal muscle, and pancreas.
Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.
Isoform 2 is phosphorylated upon DNA damage, probably by ATM or ATR at Thr-179; Ser-181 and Ser-185. Phosphorylation by HIPK2 on Ser-428 induces proteasomal degradation.
Nucleus. Cell junction > synapse.
Information by UniProt
C terminal binding protein 2 antibody
C-terminal-binding protein 2 antibody
Western blot - Anti-CTBP2 antibody [EPR7611(B)] (ab128871)
All lanes : Anti-CTBP2 antibody [EPR7611(B)] (ab128871) at 1/10000 dilution Lane 1 : HeLa cell lysate Lane 2 : SKBR-3 cell lysate Lane 3 : Saos 2 cell lysate Lysates/proteins at 10 µg per lane. Secondary All lanes : Goat-anti-rabbit HRP at 1/2000 dilution Developed using the ECL technique. Predicted band size: 49 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTBP2 antibody [EPR7611(B)] (ab128871)
ab128871 at 1/250 dilution staining CTBP2 in Paraffin-embedded Human thyroid carcinoma tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-CTBP2 antibody [EPR7611(B)] (ab128871)
ab128871 at 1/100 dilution staining CTBP2 in SKBR-3 cells by
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"