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RecombinantRabMAb

Recombinant Anti-CTBP2 antibody [EPR7611(B)] (ab128871)

Submit a review Submit a question References (9)
Immunocytochemistry/ Immunofluorescence - Anti-CTBP2 antibody [EPR7611(B)] (ab128871)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTBP2 antibody [EPR7611(B)] (ab128871)
  • Western blot - Anti-CTBP2 antibody [EPR7611(B)] (ab128871)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTBP2 antibody [EPR7611(B)] (ab128871)
  • Flow Cytometry (Intracellular) - Anti-CTBP2 antibody [EPR7611(B)] (ab128871)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTBP2 antibody [EPR7611(B)] (ab128871)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTBP2 antibody [EPR7611(B)] (ab128871)
  • Anti-CTBP2 antibody [EPR7611(B)] (ab128871)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7611(B)] to CTBP2
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free

Overview

  • Product name

    Anti-CTBP2 antibody [EPR7611(B)]
    See all CTBP2 primary antibodies

  • Description

    Rabbit monoclonal [EPR7611(B)] to CTBP2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)more details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa and SK-BR-3 cell lysates. Mouse brain and Rat brain tissue lysates. Flow Cyt (Intra): MCF7 cells. ICC/IF: SH-SY5Y cells IHC-P: Rat stomach, Mouse stomach, Human ovarian carcinoma, and Human glioma tissues.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab128871 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/10000 - 1/50000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
IHC-P
1/6000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

For mouse and rat, use at 1:12000.

For unpurified, use at 1/250 - 1/500.

See IHC antigen retrieval protocols.
ICC/IF
1/50.

For unpurified use at 1/100 - 1/250.
Flow Cyt (Intra)
1/20.
Notes
WB
1/10000 - 1/50000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
IHC-P
1/6000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

For mouse and rat, use at 1:12000.

For unpurified, use at 1/250 - 1/500.

See IHC antigen retrieval protocols.
ICC/IF
1/50.

For unpurified use at 1/100 - 1/250.
Flow Cyt (Intra)
1/20.

Target

  • Function

    Corepressor targeting diverse transcription regulators. Functions in brown adipose tissue (BAT) differentiation.
    Isoform 2 probably acts as a scaffold for specialized synapses.

  • Tissue specificity

    Ubiquitous. Highest levels in heart, skeletal muscle, and pancreas.

  • Sequence similarities

    Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.

  • Post-translational
    modifications

    Isoform 2 is phosphorylated upon DNA damage, probably by ATM or ATR at Thr-179; Ser-181 and Ser-185. Phosphorylation by HIPK2 on Ser-428 induces proteasomal degradation.

  • Cellular localization

    Nucleus. Cell junction > synapse.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • C terminal binding protein 2 antibody
    • C-terminal-binding protein 2 antibody
    • CtBP2 antibody
    • CTBP2_HUMAN antibody
    • ribeye antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-CTBP2 antibody [EPR7611(B)] (ab128871)
    Immunocytochemistry/ Immunofluorescence - Anti-CTBP2 antibody [EPR7611(B)] (ab128871)
    Immunocytochemistry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling CTBP2 with purified ab128871 at 1:50 dilution (2.5 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (2.5 µg/ml) (ab195889) (red). Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as a nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTBP2 antibody [EPR7611(B)] (ab128871)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTBP2 antibody [EPR7611(B)] (ab128871)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse stomach tissue sections labeling CTBP2 with purified ab128871 at 1:12000 (0.011 µg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. LeicaDS9800 (BondTM Polymer Refine Detection) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Western blot - Anti-CTBP2 antibody [EPR7611(B)] (ab128871)
    Western blot - Anti-CTBP2 antibody [EPR7611(B)] (ab128871)
    All lanes : Anti-CTBP2 antibody [EPR7611(B)] (ab128871) at 1/10000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : Mouse brain lysate
    Lane 4 : Rat brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 49 kDa
    Observed band size: 49 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTBP2 antibody [EPR7611(B)] (ab128871)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTBP2 antibody [EPR7611(B)] (ab128871)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue sections labeling CTBP2 with purified ab128871 at 1:6000 (0.021 µg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. LeicaDS9800 (BondTM Polymer Refine Detection) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Flow Cytometry (Intracellular) - Anti-CTBP2 antibody [EPR7611(B)] (ab128871)
    Flow Cytometry (Intracellular) - Anti-CTBP2 antibody [EPR7611(B)] (ab128871)
    Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labelling CTBP2 with purified ab128871 at 1/20 dilution (5 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150081) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (blue).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTBP2 antibody [EPR7611(B)] (ab128871)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTBP2 antibody [EPR7611(B)] (ab128871)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat stomach tissue sections labeling CTBP2 with Purified ab128871 at 1:12000 (0.011 µg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. LeicaDS9800 (BondTM Polymer Refine Detection) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTBP2 antibody [EPR7611(B)] (ab128871)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTBP2 antibody [EPR7611(B)] (ab128871)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human glioma tissue sections labeling CTBP2 with purified ab128871 at 1:6000 (0.021 µg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. LeicaDS9800 (BondTM Polymer Refine Detection) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Anti-CTBP2 antibody [EPR7611(B)] (ab128871)
    Anti-CTBP2 antibody [EPR7611(B)] (ab128871)

References (9)

Publishing research using ab128871? Please let us know so that we can cite the reference in this datasheet.

ab128871 has been referenced in 9 publications.

  • Song F  et al. Hidden hearing loss is associated with loss of ribbon synapses of cochlea inner hair cells. Biosci Rep 41:N/A (2021). PubMed: 33734328
  • Parfenyev SE  et al. Proteomic Analysis of Zeb1 Interactome in Breast Carcinoma Cells. Molecules 26:N/A (2021). PubMed: 34074001
  • Zhou P  et al. Transforming growth factor beta (TGF-ß) is activated by the CtBP2-p300-AP1 transcriptional complex in chronic renal failure. Int J Biol Sci 16:204-215 (2020). PubMed: 31929749
  • Li S  et al. FGF22 promotes generation of ribbon synapses through downregulating MEF2D. Aging (Albany NY) 12:6456-6466 (2020). PubMed: 32271716
  • Wang H  et al. Decreased levels of superoxide dismutase in inner pillar cells contribute to ribbon synapse impairment in presbycusis. Am J Transl Res 11:2403-2412 (2019). PubMed: 31105846
  • Crespo-Garcia S  et al. Individual and temporal variability of the retina after chronic bilateral common carotid artery occlusion (BCCAO). PLoS One 13:e0193961 (2018). PubMed: 29547662
  • Dai F  et al. CtBP2 overexpression promotes tumor cell proliferation and invasion in gastric cancer and is associated with poor prognosis. Oncotarget 8:28736-28749 (2017). IHC . PubMed: 28404932
  • Wang Y  et al. Expression and prognostic significance of CTBP2 in human gliomas. Oncol Lett 12:2429-2434 (2016). WB, IHC-P ; Human . PubMed: 27698809
  • Karaca E  et al. Oxygen-dependent acetylation and dimerization of the corepressor CtBP2 in neural stem cells. Exp Cell Res 332:128-35 (2015). PubMed: 25447313

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