Recombinant
RabMAb

Recombinant Anti-CTBP2 antibody [EPR7611(B)] (ab128871)

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Overview

  • Product name

    Anti-CTBP2 antibody [EPR7611(B)]
    See all CTBP2 primary antibodies

  • Description

    Rabbit monoclonal [EPR7611(B)] to CTBP2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human CTBP2 aa 400-500 (C terminal). The exact sequence is proprietary.

  • Positive control

    • HeLa, SKBR-3 and Saos 2 cell lysates; Human thyroid carcinoma tissue; SKBR-3 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab128871 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.

    • Target

    • Function

      Corepressor targeting diverse transcription regulators. Functions in brown adipose tissue (BAT) differentiation.
      Isoform 2 probably acts as a scaffold for specialized synapses.

    • Tissue specificity

      Ubiquitous. Highest levels in heart, skeletal muscle, and pancreas.

    • Sequence similarities

      Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.

    • Post-translational
      modifications

      Isoform 2 is phosphorylated upon DNA damage, probably by ATM or ATR at Thr-179; Ser-181 and Ser-185. Phosphorylation by HIPK2 on Ser-428 induces proteasomal degradation.

    • Cellular localization

      Nucleus. Cell junction > synapse.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • C terminal binding protein 2 antibody
      • C-terminal-binding protein 2 antibody
      • CtBP2 antibody
      • CTBP2_HUMAN antibody
      • ribeye antibody
      see all

    Images

    • Western blot - Anti-CTBP2 antibody [EPR7611(B)] (ab128871)
      Western blot - Anti-CTBP2 antibody [EPR7611(B)] (ab128871)
      All lanes : Anti-CTBP2 antibody [EPR7611(B)] (ab128871) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : SKBR-3 cell lysate
      Lane 3 : Saos 2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 49 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTBP2 antibody [EPR7611(B)] (ab128871)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTBP2 antibody [EPR7611(B)] (ab128871)

      ab128871 at 1/250 dilution staining CTBP2 in Paraffin-embedded Human thyroid carcinoma tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-CTBP2 antibody [EPR7611(B)] (ab128871)
      Immunocytochemistry/ Immunofluorescence - Anti-CTBP2 antibody [EPR7611(B)] (ab128871)
      ab128871 at 1/100 dilution staining CTBP2 in SKBR-3 cells by Immunofluorescence.

    References

    This product has been referenced in:

    • Wang H  et al. Decreased levels of superoxide dismutase in inner pillar cells contribute to ribbon synapse impairment in presbycusis. Am J Transl Res 11:2403-2412 (2019). Read more (PubMed: 31105846) »
    • Crespo-Garcia S  et al. Individual and temporal variability of the retina after chronic bilateral common carotid artery occlusion (BCCAO). PLoS One 13:e0193961 (2018). Read more (PubMed: 29547662) »
    See all 5 Publications for this product

    Publishing research using ab128871? Please let us know so that we can cite the reference in this datasheet.

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