Recombinant Anti-CTBP2 antibody [EPR7611(B)] - BSA and Azide free (ab248197)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7611(B)] to CTBP2 - BSA and Azide free
- Suitable for: ICC/IF, IHC-P, WB, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-CTBP2 antibody [EPR7611(B)] - BSA and Azide free
See all CTBP2 primary antibodies -
Description
Rabbit monoclonal [EPR7611(B)] to CTBP2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WB, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa and SK-BR-3 cell lysates. Mouse brain and Rat brain tissue lysates. Flow Cyt (Intra): MCF7 cells. ICC/IF: SH-SY5Y cells IHC-P: Rat stomach, Mouse stomach, Human ovarian carcinoma, and Human glioma tissues.
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General notes
ab248197 is the carrier-free version of ab128871.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.20
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7611(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-CTBP2 antibody [EPR7611(B)] (ab128871)
- Alexa Fluor® 488 Anti-CTBP2 antibody [EPR7611(B)] (ab204662)
- Alexa Fluor® 647 Anti-CTBP2 antibody [EPR7611(B)] (ab204663)
- PE Anti-CTBP2 antibody [EPR7611(B)] (ab303202)
- APC Anti-CTBP2 antibody [EPR7611(B)] (ab303203)
- HRP Anti-CTBP2 antibody [EPR7611(B)] (ab303204)
- Alexa Fluor® 594 Anti-CTBP2 antibody [EPR7611(B)] (ab310531)
- Alexa Fluor® 555 Anti-CTBP2 antibody [EPR7611(B)] (ab312061)
- Alexa Fluor® 568 Anti-CTBP2 antibody [EPR7611(B)] (ab312538)
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab248197 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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Notes |
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ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa). |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
Target
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Function
Corepressor targeting diverse transcription regulators. Functions in brown adipose tissue (BAT) differentiation.
Isoform 2 probably acts as a scaffold for specialized synapses. -
Tissue specificity
Ubiquitous. Highest levels in heart, skeletal muscle, and pancreas. -
Sequence similarities
Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. -
Post-translational
modificationsIsoform 2 is phosphorylated upon DNA damage, probably by ATM or ATR at Thr-179; Ser-181 and Ser-185. Phosphorylation by HIPK2 on Ser-428 induces proteasomal degradation. -
Cellular localization
Nucleus. Cell junction > synapse. - Information by UniProt
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Database links
- Entrez Gene: 1488 Human
- Entrez Gene: 13017 Mouse
- Entrez Gene: 81717 Rat
- Omim: 602619 Human
- SwissProt: P56545 Human
- SwissProt: P56546 Mouse
- SwissProt: Q9EQH5 Rat
- Unigene: 501345 Human
see all -
Alternative names
- C terminal binding protein 2 antibody
- C-terminal-binding protein 2 antibody
- CtBP2 antibody
see all
Images
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This data was developed using ab128871, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling CTBP2 with purified ab128871 at 1:50 dilution (2.5 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (2.5 µg/ml) (ab195889) (red). Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as a nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. -
This data was developed using ab128871, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse stomach tissue sections labeling CTBP2 with purified ab128871 at 1:12000 (0.011 µg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. LeicaDS9800 (BondTM Polymer Refine Detection) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. -
All lanes : Anti-CTBP2 antibody [EPR7611(B)] (ab128871) at 1/10000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : Mouse brain lysate
Lane 4 : Rat brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDaThis data was developed using ab128871, the same antibody clone in a different buffer formulation.
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This data was developed using ab128871, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue sections labeling CTBP2 with purified ab128871 at 1:6000 (0.021 µg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. LeicaDS9800 (BondTM Polymer Refine Detection) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. -
This data was developed using ab128871, the same antibody clone in a different buffer formulation.
Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labelling CTBP2 with purified ab128871 at 1/20 dilution (5 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150081) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (blue). -
This data was developed using ab128871, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat stomach tissue sections labeling CTBP2 with purified ab128871 at 1:12000 (0.011 µg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. LeicaDS9800 (BondTM Polymer Refine Detection) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. -
This data was developed using ab128871, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human glioma tissue sections labeling CTBP2 with purified ab128871 at 1:6000 (0.021 µg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. LeicaDS9800 (BondTM Polymer Refine Detection) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab248197 has not yet been referenced specifically in any publications.