RecombinantRabMAb

Recombinant Anti-CTBP2 antibody [EPR7611(B)] - BSA and Azide free (ab248197)

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Western blot - Anti-CTBP2 antibody [EPR7611(B)] - BSA and Azide free (ab248197)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTBP2 antibody [EPR7611(B)] - BSA and Azide free (ab248197)
  • Immunocytochemistry - Anti-CTBP2 antibody [EPR7611(B)] - BSA and Azide free (ab248197)
  • Anti-CTBP2 antibody [EPR7611(B)] - BSA and Azide free (ab248197)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7611(B)] to CTBP2 - BSA and Azide free
  • Suitable for: ICC, IHC-P, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Unconjugated

Overview

  • Product name

    Anti-CTBP2 antibody [EPR7611(B)] - BSA and Azide free
    See all CTBP2 primary antibodies

  • Description

    Rabbit monoclonal [EPR7611(B)] to CTBP2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IHC-P, WBmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab248197 is the carrier-free version of ab128871. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab248197 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab248197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
Notes
ICC
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
  • Application notes
    Is unsuitable for Flow Cyt or IP.

    • Target

    • Function

      Corepressor targeting diverse transcription regulators. Functions in brown adipose tissue (BAT) differentiation.
      Isoform 2 probably acts as a scaffold for specialized synapses.

    • Tissue specificity

      Ubiquitous. Highest levels in heart, skeletal muscle, and pancreas.

    • Sequence similarities

      Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.

    • Post-translational
      modifications

      Isoform 2 is phosphorylated upon DNA damage, probably by ATM or ATR at Thr-179; Ser-181 and Ser-185. Phosphorylation by HIPK2 on Ser-428 induces proteasomal degradation.

    • Cellular localization

      Nucleus. Cell junction > synapse.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • C terminal binding protein 2 antibody
      • C-terminal-binding protein 2 antibody
      • CtBP2 antibody
      • CTBP2_HUMAN antibody
      • ribeye antibody
      see all

    Images

    • Western blot - Anti-CTBP2 antibody [EPR7611(B)] - BSA and Azide free (ab248197)
      Western blot - Anti-CTBP2 antibody [EPR7611(B)] - BSA and Azide free (ab248197)
      All lanes : Anti-CTBP2 antibody [EPR7611(B)] (ab128871) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : SKBR-3 cell lysate
      Lane 3 : Saos 2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 49 kDa



      This data was developed using ab128871, the same antibody clone in a different buffer formulation.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTBP2 antibody [EPR7611(B)] - BSA and Azide free (ab248197)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTBP2 antibody [EPR7611(B)] - BSA and Azide free (ab248197)
      This data was developed using ab128871, the same antibody clone in a different buffer formulation.ab128871 at 1/250 dilution staining CTBP2 in Paraffin-embedded Human thyroid carcinoma tissue by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    • Immunocytochemistry - Anti-CTBP2 antibody [EPR7611(B)] - BSA and Azide free (ab248197)
      Immunocytochemistry - Anti-CTBP2 antibody [EPR7611(B)] - BSA and Azide free (ab248197)
      This data was developed using ab128871, the same antibody clone in a different buffer formulation.ab128871 at 1/100 dilution staining CTBP2 in SKBR-3 cells by Immunofluorescence.
    • Anti-CTBP2 antibody [EPR7611(B)] - BSA and Azide free (ab248197)
      Anti-CTBP2 antibody [EPR7611(B)] - BSA and Azide free (ab248197)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab248197? Please let us know so that we can cite the reference in this datasheet.

    ab248197 has not yet been referenced specifically in any publications.

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