Anti-CTC1 antibody (ab230538)
Key features and details
- Rabbit polyclonal to CTC1
- Suitable for: IHC-P, WB, Flow Cyt (Intra)
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CTC1 antibody -
Description
Rabbit polyclonal to CTC1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human CTC1 aa 767-795 (internal sequence) conjugated to keyhole limpet haemocyanin.
Database link: Q2NKJ3 -
Positive control
- WB: K562 whole cell lysate. IHC-P: Human kidney tissue. Flow Cyt (Intra): K562 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab230538 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB |
1/1000. Predicted molecular weight: 135 kDa.
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Flow Cyt (Intra) |
1/10 - 1/50.
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Notes |
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IHC-P
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
1/1000. Predicted molecular weight: 135 kDa. |
Flow Cyt (Intra)
1/10 - 1/50. |
Target
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Function
Component of the CST complex, a complex that binds to single-stranded DNA and is required to protect telomeres from DNA degradation. The CST complex binds single-stranded DNA with high affinity in a sequence-independent manner, while isolated subunits bind DNA with low affinity by themselves. In addition to telomere protection, the CST complex has probably a more general role in DNA metabolism at non-telomeric sites. -
Involvement in disease
Cerebroretinal microangiopathy with calcifications and cysts -
Sequence similarities
Belongs to the CTC1 family. -
Cellular localization
Nucleus. Chromosome > telomere. A transmembrane region is predicted by sequence analysis tools (ESKW, MEMSAT and Phobius); however, given the telomeric localization of the protein, the relevance of the transmembrane region is unsure in vivo. - Information by UniProt
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Database links
- Entrez Gene: 80169 Human
- Omim: 613129 Human
- SwissProt: Q2NKJ3 Human
- Unigene: 156055 Human
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Alternative names
- CTC1 antibody
- C17orf68 antibody
- Conserved telomere maintenance component 1 antibody
see all
Images
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Formalin-fixed, parafin-embedded human kidney tissue stained for CTC1 using ab230538 at 1/50 dilution in immunohistochemical analysis.
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Anti-CTC1 antibody (ab230538) at 1/1000 dilution + K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 135 kDa -
Intracellular flow cytometric analysis ofK562 (human chronic myelogenous leukemia cell line from bone marrow) cell line labeling CTC1 with ab230538 at 1/10 dilution (green) compared with a negative control (blue). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (2)
ab230538 has been referenced in 2 publications.
- Lyu X et al. Human CST complex protects stalled replication forks by directly blocking MRE11 degradation of nascent-strand DNA. EMBO J 40:e103654 (2021). PubMed: 33210317
- Lei KH et al. Crosstalk between CST and RPA regulates RAD51 activity during replication stress. Nat Commun 12:6412 (2021). PubMed: 34741010