Overview

  • Product name

  • Description

    Goat polyclonal to CTCF
  • Host species

    Goat
  • Specificity

    ab223224 is expected to recognize reported isoform 1 (NP_006556.1) only.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to Human CTCF aa 208-220 (internal sequence) (Cysteine residue). (NP_006556.1).
    Sequence:

    CKKSKLRYTEEGKD


    Database link: P49711

  • Positive control

    • WB: Jurkat cell and nuclear lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab223224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.03 - 0.3 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 83 kDa).

Target

  • Function

    Chromatin binding factor that binds to DNA sequence specific sites. Involved in transcriptional regulation by binding to chromatin insulators and preventing interaction between promoter and nearby enhancers and silencers. Acts as transcriptional repressor binding to promoters of vertebrate MYC gene and BAG1 gene. Also binds to the PLK and PIM1 promoters. Acts as a transcriptional activator of APP. Regulates APOA1/C3/A4/A5 gene cluster and controls MHC class II gene expression. Plays an essential role in oocyte and preimplantation embryo development by activating or repressing transcription. Seems to act as tumor suppressor. Plays a critical role in the epigenetic regulation. Participates to the allele-specific gene expression at the imprinted IGF2/H19 gene locus. On the maternal allele, binding within the H19 imprinting control region (ICR) mediates maternally inherited higher-order chromatin conformation to restrict enhancer access to IGF2. Plays a critical role in gene silencing over considerable distances in the genome. Preferentially interacts with unmethylated DNA, preventing spreading of CpG methylation and maintaining methylation-free zones. Inversely, binding to target sites is prevented by CpG methylation. Plays a important role in chromatin remodeling. Can dimerize when it is bound to different DNA sequences, mediating long-range chromatin looping. Mediates interchromosomal association between IGF2/H19 and WSB1/NF1 and may direct distant DNA segments to a common transcription factory. Causes local loss of histone acetylation and gain of histone methylation in the beta-globin locus, without affecting transcription. When bound to chromatin, it provides an anchor point for nucleosomes positioning. Seems to be essential for homologous X-chromosome pairing. May participate with Tsix in establishing a regulatable epigenetic switch for X chromosome inactivation. May play a role in preventing the propagation of stable methylation at the escape genes from X- inactivation. Involved in sister chromatid cohesion. Associates with both centromeres and chromosomal arms during metaphase and required for cohesin localization to CTCF sites. Regulates asynchronous replication of IGF2/H19.
  • Tissue specificity

    Ubiquitous. Absent in primary spermatocytes.
  • Sequence similarities

    Belongs to the CTCF zinc-finger protein family.
    Contains 11 C2H2-type zinc fingers.
  • Domain

    The 11 zinc fingers are highly conserved among vertebrates, exhibiting almost identical amino acid sequences. Different subsets or combination of individual zinc fingers gives the ability to CTCF to recognize multiple DNA target sites.
  • Post-translational
    modifications

    Sumoylated on Lys-74 and Lys-689; sumoylation of CTCF contributes to the repressive function of CTCF on the MYC P2 promoter.
  • Cellular localization

    Nucleus > nucleoplasm. Chromosome. Chromosome > centromere. May translocate to the nucleolus upon cell differentiation. Associates with both centromeres and chromosomal arms during metaphase. Associates with the H19 ICR in mitotic chromosomes. May be preferentially excluded from heterochromatin during interphase.
  • Information by UniProt
  • Database links

  • Alternative names

    • 11 zinc finger protein antibody
    • 11 zinc finger transcriptional repressor antibody
    • 11-zinc finger protein antibody
    • CCCTC binding factor (zinc finger protein) antibody
    • CCCTC binding factor antibody
    • CCCTC-binding factor antibody
    • Ctcf antibody
    • CTCF_HUMAN antibody
    • CTCFL paralog antibody
    • MRD21 antibody
    • Transcriptional repressor CTCF antibody
    see all

Images

  • All lanes : Anti-CTCF antibody (ab223224) at 0.1 µg/ml

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate (in RIPA buffer).
    Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) nuclear lysate (in RIPA buffer).

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 83 kDa
    Observed band size: 150 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour.

References

ab223224 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Chicken Cell lysate - whole cell (DF-1 (fibroblast spontaneously transformed from ch)
Gel Running Conditions
Non-reduced Denaturing (10% (Fast-cast stain -free - Biorad))
Loading amount
30 µg
Specification
DF-1 (fibroblast spontaneously transformed from ch
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

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Verified customer

Submitted Oct 02 2017

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