Anti-CTCF antibody (ab70303)
Key features and details
- Rabbit polyclonal to CTCF
- Suitable for: IHC-Fr, WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-CTCF antibody
See all CTCF primary antibodies -
Description
Rabbit polyclonal to CTCF -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Fr, WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
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Immunogen
Synthetic peptide corresponding to Human CTCF aa 650-750.
Database link: P49711 -
Positive control
- IP: Jurkat whole cell lysate. IHC-P: Human lung cancer tissue, mouse renal cancer tissue. WB: Jurkat, HEK293T and HeLa whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab70303 was affinity purified using an epitope specific to CTCF immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70303 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | (1) |
Use at an assay dependent concentration.
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| WB | (5) |
1/2500 - 1/10000.
Detects a band of approximately 100 kDa (predicted molecular weight: 83 kDa). |
| IP |
Use at 2-5 µg/mg of lysate.
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| IHC-P | (1) |
1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| Notes |
|---|
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IHC-Fr
Use at an assay dependent concentration. |
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WB
1/2500 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 83 kDa). |
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IP
Use at 2-5 µg/mg of lysate. |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Chromatin binding factor that binds to DNA sequence specific sites. Involved in transcriptional regulation by binding to chromatin insulators and preventing interaction between promoter and nearby enhancers and silencers. Acts as transcriptional repressor binding to promoters of vertebrate MYC gene and BAG1 gene. Also binds to the PLK and PIM1 promoters. Acts as a transcriptional activator of APP. Regulates APOA1/C3/A4/A5 gene cluster and controls MHC class II gene expression. Plays an essential role in oocyte and preimplantation embryo development by activating or repressing transcription. Seems to act as tumor suppressor. Plays a critical role in the epigenetic regulation. Participates to the allele-specific gene expression at the imprinted IGF2/H19 gene locus. On the maternal allele, binding within the H19 imprinting control region (ICR) mediates maternally inherited higher-order chromatin conformation to restrict enhancer access to IGF2. Plays a critical role in gene silencing over considerable distances in the genome. Preferentially interacts with unmethylated DNA, preventing spreading of CpG methylation and maintaining methylation-free zones. Inversely, binding to target sites is prevented by CpG methylation. Plays a important role in chromatin remodeling. Can dimerize when it is bound to different DNA sequences, mediating long-range chromatin looping. Mediates interchromosomal association between IGF2/H19 and WSB1/NF1 and may direct distant DNA segments to a common transcription factory. Causes local loss of histone acetylation and gain of histone methylation in the beta-globin locus, without affecting transcription. When bound to chromatin, it provides an anchor point for nucleosomes positioning. Seems to be essential for homologous X-chromosome pairing. May participate with Tsix in establishing a regulatable epigenetic switch for X chromosome inactivation. May play a role in preventing the propagation of stable methylation at the escape genes from X- inactivation. Involved in sister chromatid cohesion. Associates with both centromeres and chromosomal arms during metaphase and required for cohesin localization to CTCF sites. Regulates asynchronous replication of IGF2/H19. -
Tissue specificity
Ubiquitous. Absent in primary spermatocytes. -
Sequence similarities
Belongs to the CTCF zinc-finger protein family.
Contains 11 C2H2-type zinc fingers. -
Domain
The 11 zinc fingers are highly conserved among vertebrates, exhibiting almost identical amino acid sequences. Different subsets or combination of individual zinc fingers gives the ability to CTCF to recognize multiple DNA target sites. -
Post-translational
modificationsSumoylated on Lys-74 and Lys-689; sumoylation of CTCF contributes to the repressive function of CTCF on the MYC P2 promoter. -
Cellular localization
Nucleus > nucleoplasm. Chromosome. Chromosome > centromere. May translocate to the nucleolus upon cell differentiation. Associates with both centromeres and chromosomal arms during metaphase. Associates with the H19 ICR in mitotic chromosomes. May be preferentially excluded from heterochromatin during interphase. - Information by UniProt
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Database links
- Entrez Gene: 517097 Cow
- Entrez Gene: 10664 Human
- Entrez Gene: 13018 Mouse
- Entrez Gene: 100623274 Pig
- Entrez Gene: 83726 Rat
- Omim: 604167 Human
- SwissProt: P49711 Human
- SwissProt: Q61164 Mouse
see all -
Alternative names
- 11 zinc finger protein antibody
- 11 zinc finger transcriptional repressor antibody
- 11-zinc finger protein antibody
see all
Images
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Immunoprecipitation - Anti-CTCF antibody (ab70303)
CTCF was immunoprecipitated from 1 mg Jurkat whole cell lysate with ab70303 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab70303 at 0.02 µg/mL.
Lane 1: ab70303 IP in Jurkat whole cell lysate.
Lane 2: Contol IgG in Jurkat whole cell lysate.
Detection: Chemiluminescence.
Exposure time: 10 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTCF antibody (ab70303)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human small cell lung cancer tissue, labeling CTCF with ab70303 at a 1/1000 dilution. HRP-conjugated goat anti-rabbit IgG was used as a secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTCF antibody (ab70303)Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse renal cancer tissue, labeling CTCF with ab70303 at a 1/1000 dilution. HRP-conjugated goat anti-rabbit IgG was used as a secondary antibody.
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Western blot - Anti-CTCF antibody (ab70303)All lanes : Anti-CTCF antibody (ab70303) at 0.02 µg/ml
Lane 1 : Jurkat whole cell lysate
Lane 2 : HEK293T whole cell lysate
Lane 3 : HeLa whole cell lysate
Lysates/proteins at 50 µg per lane.
Exposure time: 10 secondsDetection: Chemiluminescence.Lysates prepared using NETN lysis buffer.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (63)
ab70303 has been referenced in 63 publications.
- Li X et al. Cohesin promotes HSV-1 lytic transcription by facilitating the binding of RNA Pol II on viral genes. Virol J 18:26 (2021). PubMed: 33485391
- Laffleur B et al. Noncoding RNA processing by DIS3 regulates chromosomal architecture and somatic hypermutation in B cells. Nat Genet 53:230-242 (2021). PubMed: 33526923
- Elder EG et al. Regulation of host and viral promoters during human cytomegalovirus latency via US28 and CTCF. J Gen Virol 102:N/A (2021). PubMed: 34042564
- Xu Y et al. Sp1 Targeted PARP1 Inhibition Protects Cardiomyocytes From Myocardial Ischemia-Reperfusion Injury via Downregulation of Autophagy. Front Cell Dev Biol 9:621906 (2021). PubMed: 34124031
- Xu B et al. Acute depletion of CTCF rewires genome-wide chromatin accessibility. Genome Biol 22:244 (2021). PubMed: 34429148