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  1. Link

    ctcf-antibody-ab70303.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger
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Anti-CTCF antibody (ab70303)

  • Datasheet
  • SDS
Reviews (12)Q&A (9)References (63)

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Immunoprecipitation - Anti-CTCF antibody (ab70303)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTCF antibody (ab70303)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTCF antibody (ab70303)
  • Western blot - Anti-CTCF antibody (ab70303)

Key features and details

  • Rabbit polyclonal to CTCF
  • Suitable for: IHC-Fr, WB, IP, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-CTCF antibody [EPR18253] - ChIP Grade (ab188408)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-CTCF antibody
    See all CTCF primary antibodies
  • Description

    Rabbit polyclonal to CTCF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Fr, WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to Human CTCF aa 650-750.
    Database link: P49711

  • Positive control

    • IP: Jurkat whole cell lysate. IHC-P: Human lung cancer tissue, mouse renal cancer tissue. WB: Jurkat, HEK293T and HeLa whole cell lysate.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab70303 was affinity purified using an epitope specific to CTCF immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding
    • Epigenetics and Nuclear Signaling
    • DNA methylation
    • Methylated DNA Associated

Associated products

  • ChIP Related Products

    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • NIH/3T3 whole cell lysate (ab7179)
  • Recombinant Protein

    • Recombinant Human CTCF protein (ab153114)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab70303 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr (1)
Use at an assay dependent concentration.
WB (5)
1/2500 - 1/10000.

Detects a band of approximately 100 kDa (predicted molecular weight: 83 kDa).

IP
Use at 2-5 µg/mg of lysate.
IHC-P (1)
1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
IHC-Fr
Use at an assay dependent concentration.
WB
1/2500 - 1/10000.

Detects a band of approximately 100 kDa (predicted molecular weight: 83 kDa).

IP
Use at 2-5 µg/mg of lysate.
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Chromatin binding factor that binds to DNA sequence specific sites. Involved in transcriptional regulation by binding to chromatin insulators and preventing interaction between promoter and nearby enhancers and silencers. Acts as transcriptional repressor binding to promoters of vertebrate MYC gene and BAG1 gene. Also binds to the PLK and PIM1 promoters. Acts as a transcriptional activator of APP. Regulates APOA1/C3/A4/A5 gene cluster and controls MHC class II gene expression. Plays an essential role in oocyte and preimplantation embryo development by activating or repressing transcription. Seems to act as tumor suppressor. Plays a critical role in the epigenetic regulation. Participates to the allele-specific gene expression at the imprinted IGF2/H19 gene locus. On the maternal allele, binding within the H19 imprinting control region (ICR) mediates maternally inherited higher-order chromatin conformation to restrict enhancer access to IGF2. Plays a critical role in gene silencing over considerable distances in the genome. Preferentially interacts with unmethylated DNA, preventing spreading of CpG methylation and maintaining methylation-free zones. Inversely, binding to target sites is prevented by CpG methylation. Plays a important role in chromatin remodeling. Can dimerize when it is bound to different DNA sequences, mediating long-range chromatin looping. Mediates interchromosomal association between IGF2/H19 and WSB1/NF1 and may direct distant DNA segments to a common transcription factory. Causes local loss of histone acetylation and gain of histone methylation in the beta-globin locus, without affecting transcription. When bound to chromatin, it provides an anchor point for nucleosomes positioning. Seems to be essential for homologous X-chromosome pairing. May participate with Tsix in establishing a regulatable epigenetic switch for X chromosome inactivation. May play a role in preventing the propagation of stable methylation at the escape genes from X- inactivation. Involved in sister chromatid cohesion. Associates with both centromeres and chromosomal arms during metaphase and required for cohesin localization to CTCF sites. Regulates asynchronous replication of IGF2/H19.
  • Tissue specificity

    Ubiquitous. Absent in primary spermatocytes.
  • Sequence similarities

    Belongs to the CTCF zinc-finger protein family.
    Contains 11 C2H2-type zinc fingers.
  • Domain

    The 11 zinc fingers are highly conserved among vertebrates, exhibiting almost identical amino acid sequences. Different subsets or combination of individual zinc fingers gives the ability to CTCF to recognize multiple DNA target sites.
  • Post-translational
    modifications

    Sumoylated on Lys-74 and Lys-689; sumoylation of CTCF contributes to the repressive function of CTCF on the MYC P2 promoter.
  • Cellular localization

    Nucleus > nucleoplasm. Chromosome. Chromosome > centromere. May translocate to the nucleolus upon cell differentiation. Associates with both centromeres and chromosomal arms during metaphase. Associates with the H19 ICR in mitotic chromosomes. May be preferentially excluded from heterochromatin during interphase.
  • Target information above from: UniProt accession P49711 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 517097 Cow
    • Entrez Gene: 10664 Human
    • Entrez Gene: 13018 Mouse
    • Entrez Gene: 100623274 Pig
    • Entrez Gene: 83726 Rat
    • Omim: 604167 Human
    • SwissProt: P49711 Human
    • SwissProt: Q61164 Mouse
    • SwissProt: Q9R1D1 Rat
    • Unigene: 368367 Human
    • Unigene: 269474 Mouse
    • Unigene: 18612 Rat
    see all
  • Alternative names

    • 11 zinc finger protein antibody
    • 11 zinc finger transcriptional repressor antibody
    • 11-zinc finger protein antibody
    • CCCTC binding factor (zinc finger protein) antibody
    • CCCTC binding factor antibody
    • CCCTC-binding factor antibody
    • Ctcf antibody
    • CTCF_HUMAN antibody
    • CTCFL paralog antibody
    • MRD21 antibody
    • Transcriptional repressor CTCF antibody
    see all

Images

  • Immunoprecipitation - Anti-CTCF antibody (ab70303)
    Immunoprecipitation - Anti-CTCF antibody (ab70303)

    CTCF was immunoprecipitated from 1 mg Jurkat whole cell lysate with ab70303 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab70303 at 0.02 µg/mL.

    Lane 1: ab70303 IP in Jurkat whole cell lysate.

    Lane 2: Contol IgG in Jurkat whole cell lysate.

    Detection: Chemiluminescence.

    Exposure time: 10 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTCF antibody (ab70303)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTCF antibody (ab70303)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded human small cell lung cancer tissue, labeling CTCF with ab70303 at a 1/1000 dilution. HRP-conjugated goat anti-rabbit IgG was used as a secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTCF antibody (ab70303)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTCF antibody (ab70303)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse renal cancer tissue, labeling CTCF with ab70303 at a 1/1000 dilution. HRP-conjugated goat anti-rabbit IgG was used as a secondary antibody.

  • Western blot - Anti-CTCF antibody (ab70303)
    Western blot - Anti-CTCF antibody (ab70303)
    All lanes : Anti-CTCF antibody (ab70303) at 0.02 µg/ml

    Lane 1 : Jurkat whole cell lysate
    Lane 2 : HEK293T whole cell lysate
    Lane 3 : HeLa whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Exposure time: 10 seconds


    Detection: Chemiluminescence.
     
    Lysates prepared using NETN lysis buffer.

Protocols

  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (63)

Publishing research using ab70303? Please let us know so that we can cite the reference in this datasheet.

ab70303 has been referenced in 63 publications.

  • Li X  et al. Cohesin promotes HSV-1 lytic transcription by facilitating the binding of RNA Pol II on viral genes. Virol J 18:26 (2021). PubMed: 33485391
  • Laffleur B  et al. Noncoding RNA processing by DIS3 regulates chromosomal architecture and somatic hypermutation in B cells. Nat Genet 53:230-242 (2021). PubMed: 33526923
  • Elder EG  et al. Regulation of host and viral promoters during human cytomegalovirus latency via US28 and CTCF. J Gen Virol 102:N/A (2021). PubMed: 34042564
  • Xu Y  et al. Sp1 Targeted PARP1 Inhibition Protects Cardiomyocytes From Myocardial Ischemia-Reperfusion Injury via Downregulation of Autophagy. Front Cell Dev Biol 9:621906 (2021). PubMed: 34124031
  • Xu B  et al. Acute depletion of CTCF rewires genome-wide chromatin accessibility. Genome Biol 22:244 (2021). PubMed: 34429148
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-10 of 21 Abreviews or Q&A

Western blot abreview for Anti-CTCF antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse vascular smooth muscle cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
35 µg
Specification
Mouse vascular smooth muscle cells
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More

Abcam user community

Verified customer

Submitted Jan 04 2022

Western blot abreview for Anti-CTCF antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
U2OS
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More

Abcam user community

Verified customer

Submitted Jan 04 2022

Immunocytochemistry/ Immunofluorescence abreview for Anti-CTCF antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mouse vascular smooth muscle cells)
Permeabilization
Yes - NP40
Specification
Mouse vascular smooth muscle cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Jan 04 2022

Western blot abreview for Anti-CTCF antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
15 µg
Specification
Heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Aug 19 2020

Immunohistochemistry (Frozen sections) abreview for Anti-CTCF antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Heart)
Permeabilization
Yes - 0.2%Tween-20 in PBS
Specification
Heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Aug 14 2020

Western blot abreview for Anti-CTCF antibody - ChIP Grade

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (DLD1)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
20 µg
Specification
DLD1
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Mar 14 2017

ChIP abreview for Anti-CTCF antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
ChIP
Sample
Human Cell lysate - nuclear (B Cells)
Negative control
IgG
Specification
B Cells
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 5 minute(s) and 0 second(s)
Specification of the cross-linking agent: Methanol free Formaldehyde
Positive control
Gene specific promoter
Read More

Abcam user community

Verified customer

Submitted Feb 24 2017

Immunocytochemistry/ Immunofluorescence abreview for Anti-CTCF antibody - ChIP Grade

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS)
Permeabilization
Yes - Methanol
Specification
U2OS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted May 05 2015

Question

Customer kindly contacted us regarding positve and negative primers for our ChIP grade antibodies.

Read More

Abcam community

Verified customer

Asked on Feb 06 2013

Answer

I've compiled some information below regarding potential primer sets for the ChIP antibodies we talked about, where information was lacking (verified in ChIP by other researches), I included links to abReviews and references that used the antibody in ChIP.

1. ab817: GAPDH (+), GAD1 (-), AFM (-), Factor VIII (-)

2. ab1791: GAPDH, RPL30, ALDOA, MYO-D, SERPINA, AFM, SAT2, SATa (each batch is verified against this primer set, an example image of one batch test is given on the datasheet)

3. ab7030: was verified in ChIP by other researchers submitting data through our abReview system and specific references. Their positive and negative controls used are given through the link below, as well as a "click to contact this user" link that allows you to contact the researchers if you would like more specific primer information. The specific references with ChIP listed in bold are good sources for positive and negative primers as well.
a) Abreview: (NFKBIA promoter (+), GAPDH (-)) https://www.abcam.com/HDAC3-antibody-ChIP-Grade-ab7030-reviews.html?intabreviewid=25057
b) Abreview: https://www.abcam.com/HDAC3-antibody-ChIP-Grade-ab7030-reviews.html?intabreviewid=18386
c) Specific references: https://www.abcam.com/HDAC3-antibody-ChIP-Grade-ab7030-references.html

4. ab111069 Untested in ChIP.

5. ab992: was verified in ChIP by other researchers (abReviews and specific references)
a) Specific references: https://www.abcam.com/Rad21-antibody-ChIP-Grade-ab992-references.html
b) Abreview: https://www.abcam.com/Rad21-antibody-ChIP-Grade-ab992-reviews.html?intabreviewid=14542
c) Abreview: (EBV 3+ (+), EBV1,2,4,5,6 (-), GAPDH (-), Cen (-)) https://www.abcam.com/Rad21-antibody-ChIP-Grade-ab992-reviews.html?intabreviewid=33942

Our ChIP primer sets:
https://www.abcam.com/index.html?pageconfig=resource&rid=10600

Read More

Abcam Scientific Support

Answered on Feb 06 2013

Question

I received this product and it was hot when I opened it. I was advised to test it, but when I did it did not work.
Used in WB: tested various conditions for blocking and dilutions. Still no signal. I would like a replacement.

Read More

Abcam community

Verified customer

Asked on Sep 24 2012

Answer

Thank you for contacting Abcam regarding ab70303.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

Abcam Scientific Support

Answered on Sep 24 2012

1-10 of 21 Abreviews or Q&A

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