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Synthetic peptide corresponding to a region between residues 650 and 700 of human CTCF (Peptide available as ab115042.)
Our Abpromise guarantee covers the use of ab70303 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2500 - 1/10000.
Detects a band of approximately 100 kDa (predicted molecular weight: 83 kDa).
|IP||Use at 2-5 µg/mg of lysate.|
|ChIP||Use at an assay dependent concentration.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|CHIPseq||Use at an assay dependent concentration. PubMed: 28945252|
|EMSA||Use at an assay dependent concentration. PubMed: 22210889|
Samples: Whole cell lysate (50 µg) from CT26.WT, 4T1, Renca, and TCMK-1 cells. ab70303 used at a dilution of 0.2 µg/ml.
Detection: Chemiluminescence with an exposure time of 30 seconds.
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