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Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger
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Anti-CTCF antibody [mAbcam 37477] (ab37477)

  • Datasheet
Submit a review Submit a question References (4)

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Western blot - Anti-CTCF antibody [mAbcam 37477] (ab37477)
  • Western blot - Anti-CTCF antibody [mAbcam 37477] (ab37477)
  • Flow Cytometry - Anti-CTCF antibody [mAbcam 37477] (ab37477)

Key features and details

  • Mouse monoclonal [mAbcam 37477] to CTCF
  • Suitable for: Flow Cyt, WB
  • Reacts with: Human
  • Isotype: IgG1

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Secondary
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Goat Anti-Mouse IgG H&L (HRP) (ab205719)

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Overview

  • Product name

    Anti-CTCF antibody [mAbcam 37477]
    See all CTCF primary antibodies
  • Description

    Mouse monoclonal [mAbcam 37477] to CTCF
  • Host species

    Mouse
  • Specificity

    This antibody has previously been successfully used in western blot after immunoprecipitation from mouse NIH3T3 lysates. Please see Yao H et al. Genes Dev 24:2543-55 (2010) PMID: 20966046 for more details.
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to Human CTCF aa 1-300 (N terminal).

  • Positive control

    • This antibody gave a positive signal in MCF7 whole cell lysate and HeLa whole cell lysate.
  • General notes

    This antibody clone is manufactured by Abcam.

    CTCF is known to migrate by SDS-PAGE at 130 kDa (PMID: 9016583) We can conjugate this antibody to FITC for you (please see ab150242 for details).

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    IgG fraction
  • Clonality

    Monoclonal
  • Clone number

    mAbcam 37477
  • Myeloma

    Sp2/0-Ag14
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding
    • Epigenetics and Nuclear Signaling
    • DNA methylation
    • Methylated DNA Associated

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Recombinant Protein

    • Recombinant Human CTCF protein (ab153114)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab37477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
WB
Human
All applications
Mouse
Application Abreviews Notes
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

WB
Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 83 kDa).
Notes
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

WB
Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 83 kDa).

Target

  • Function

    Chromatin binding factor that binds to DNA sequence specific sites. Involved in transcriptional regulation by binding to chromatin insulators and preventing interaction between promoter and nearby enhancers and silencers. Acts as transcriptional repressor binding to promoters of vertebrate MYC gene and BAG1 gene. Also binds to the PLK and PIM1 promoters. Acts as a transcriptional activator of APP. Regulates APOA1/C3/A4/A5 gene cluster and controls MHC class II gene expression. Plays an essential role in oocyte and preimplantation embryo development by activating or repressing transcription. Seems to act as tumor suppressor. Plays a critical role in the epigenetic regulation. Participates to the allele-specific gene expression at the imprinted IGF2/H19 gene locus. On the maternal allele, binding within the H19 imprinting control region (ICR) mediates maternally inherited higher-order chromatin conformation to restrict enhancer access to IGF2. Plays a critical role in gene silencing over considerable distances in the genome. Preferentially interacts with unmethylated DNA, preventing spreading of CpG methylation and maintaining methylation-free zones. Inversely, binding to target sites is prevented by CpG methylation. Plays a important role in chromatin remodeling. Can dimerize when it is bound to different DNA sequences, mediating long-range chromatin looping. Mediates interchromosomal association between IGF2/H19 and WSB1/NF1 and may direct distant DNA segments to a common transcription factory. Causes local loss of histone acetylation and gain of histone methylation in the beta-globin locus, without affecting transcription. When bound to chromatin, it provides an anchor point for nucleosomes positioning. Seems to be essential for homologous X-chromosome pairing. May participate with Tsix in establishing a regulatable epigenetic switch for X chromosome inactivation. May play a role in preventing the propagation of stable methylation at the escape genes from X- inactivation. Involved in sister chromatid cohesion. Associates with both centromeres and chromosomal arms during metaphase and required for cohesin localization to CTCF sites. Regulates asynchronous replication of IGF2/H19.
  • Tissue specificity

    Ubiquitous. Absent in primary spermatocytes.
  • Sequence similarities

    Belongs to the CTCF zinc-finger protein family.
    Contains 11 C2H2-type zinc fingers.
  • Domain

    The 11 zinc fingers are highly conserved among vertebrates, exhibiting almost identical amino acid sequences. Different subsets or combination of individual zinc fingers gives the ability to CTCF to recognize multiple DNA target sites.
  • Post-translational
    modifications

    Sumoylated on Lys-74 and Lys-689; sumoylation of CTCF contributes to the repressive function of CTCF on the MYC P2 promoter.
  • Cellular localization

    Nucleus > nucleoplasm. Chromosome. Chromosome > centromere. May translocate to the nucleolus upon cell differentiation. Associates with both centromeres and chromosomal arms during metaphase. Associates with the H19 ICR in mitotic chromosomes. May be preferentially excluded from heterochromatin during interphase.
  • Target information above from: UniProt accession P49711 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10664 Human
    • Entrez Gene: 13018 Mouse
    • Omim: 604167 Human
    • SwissProt: P49711 Human
    • SwissProt: Q61164 Mouse
    • Unigene: 368367 Human
    • Unigene: 269474 Mouse
    • Alternative names

      • 11 zinc finger protein antibody
      • 11 zinc finger transcriptional repressor antibody
      • 11-zinc finger protein antibody
      • CCCTC binding factor (zinc finger protein) antibody
      • CCCTC binding factor antibody
      • CCCTC-binding factor antibody
      • Ctcf antibody
      • CTCF_HUMAN antibody
      • CTCFL paralog antibody
      • MRD21 antibody
      • Transcriptional repressor CTCF antibody
      see all

    Images

    • Western blot - Anti-CTCF antibody [mAbcam 37477] (ab37477)
      Western blot - Anti-CTCF antibody [mAbcam 37477] (ab37477)
      All lanes : Anti-CTCF antibody [mAbcam 37477] (ab37477) at 5 µg/ml

      Lane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
      Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 83 kDa


      Exposure time: 90 seconds
    • Western blot - Anti-CTCF antibody [mAbcam 37477] (ab37477)
      Western blot - Anti-CTCF antibody [mAbcam 37477] (ab37477)This image is courtesy of Elena Klenova, University of Essex, UK
      All lanes : Anti-CTCF antibody [mAbcam 37477] (ab37477) at 2 µg/ml

      Lane 1 : ZR-75-1 cell extract
      Lane 2 : HCT-116 cell extract

      Predicted band size: 83 kDa



      ab37477 recognises a band at approximately 130 kDa corresponding to CTCF.
    • Flow Cytometry - Anti-CTCF antibody [mAbcam 37477] (ab37477)
      Flow Cytometry - Anti-CTCF antibody [mAbcam 37477] (ab37477)
      Overlay histogram showing HeLa cells stained with ab37477 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab37477, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Protocols

    • Flow cytometry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (4)

    Publishing research using ab37477? Please let us know so that we can cite the reference in this datasheet.

    ab37477 has been referenced in 4 publications.

    • Wiechens N  et al. The Chromatin Remodelling Enzymes SNF2H and SNF2L Position Nucleosomes adjacent to CTCF and Other Transcription Factors. PLoS Genet 12:e1005940 (2016). PubMed: 27019336
    • Huang K  et al. Ribosomal RNA gene transcription mediated by the master genome regulator protein CCCTC-binding factor (CTCF) is negatively regulated by the condensin complex. J Biol Chem 288:26067-77 (2013). WB . PubMed: 23884423
    • Golan-Mashiach M  et al. Identification of CTCF as a master regulator of the clustered protocadherin genes. Nucleic Acids Res : (2011). EMSA . PubMed: 22210889
    • Yao H  et al. Mediation of CTCF transcriptional insulation by DEAD-box RNA-binding protein p68 and steroid receptor RNA activator SRA. Genes Dev 24:2543-55 (2010). WB, IP ; Human, Mouse . PubMed: 20966046

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