Key features and details
- Mouse monoclonal [OTI7F5] to CTDSP2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-CTDSP2 antibody [OTI7F5]
See all CTDSP2 primary antibodies
DescriptionMouse monoclonal [OTI7F5] to CTDSP2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Recombinant full length protein corresponding to Human CTDSP2 aa 1-271.
MEHGSIITQARREDALVLTKQGLVSKSSPKKPRGRNIFKALFCCFRAQHV GQSSSSTELAAYKEEANTIAKSDLLQCLQYQFYQIPGTCLLPEVTEEDQG RICVVIDLDETLVHSSFKPINNADFIVPIEIEGTTHQVYVLKRPYVDEFL RRMGELFECVLFTASLAKYADPVTDLLDRCGVFRARLFRESCVFHQGCYV KDLSRLGRDLRKTLILDNSPASYIFHPENAVPVQSWFDDMADTELLNLIP IFEELSGAEDVYTSLGQLRAP
Database link: O14595
- IHC-P: Human ovary adenocarcinoma tissue. WB: pCMV6-ENTRY CTDSP2 cDNA transfected HEK-293T cell lysate.
Clone OTI7F5 (formerly 7F5).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purification notesPurified from cell culture supernatant.
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
Our Abpromise guarantee covers the use of ab236379 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 31 kDa.|
Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 minutes.
FunctionPreferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residues repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation. Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells. May contribute to the development of sarcomas.
Tissue specificityExpression is restricted to non-neuronal tissues. Highest expression in pancreas and lowest in liver.
Sequence similaritiesContains 1 FCP1 homology domain.
- Information by UniProt
- Carboxy terminal domain RNA polymerase II polypeptide A small phosphatase 2 antibody
- Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 2 antibody
- conserved gene amplified in osteosarcoma 4 antibody
Paraffin-embedded human ovary adenocarcinoma tissue stained for CTDSP2 with ab236379 at a 1/150 dilution in immunohistochemical analysis.
All lanes : Anti-CTDSP2 antibody [OTI7F5] (ab236379) at 1/2000 dilution
Lane 1 : pCMV6-ENTRY control cDNA transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : pCMV6-ENTRY CTDSP2 cDNA transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 31 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236379 has not yet been referenced specifically in any publications.