Product nameAnti-CTF18 antibody [2182C3a]
See all CTF18 primary antibodies
DescriptionMouse monoclonal [2182C3a] to CTF18
Tested applicationsSuitable for: WB, IP, Dot blotmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human CTF18.
- HT1080 whole cell lysate.
Previously labelled as CHTF18.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride, PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab51331 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at 10µg/mg RIPA lysate of HeLa cells.
WB: 1/100. Detects a band of approximately 118 kDa (predicted molecular weight: 107 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionChromosome cohesion factor involved in sister chromatid cohesion and fidelity of chromosome transmission. Component of one of the cell nuclear antigen loader complexes, CTF18-replication factor C (CTF18-RFC), which consists of CTF18, CTF8, DCC1, RFC2, RFC3, RFC4 and RFC5. The CTF18-RFC complex binds to single-stranded and primed DNAs and has weak ATPase activity that is stimulated by the presence of primed DNA, replication protein A (RPA) and by proliferating cell nuclear antigen (PCNA). The CTF18-RFC complex catalyzes the ATP-dependent loading of PCNA onto primed and gapped DNA. It also interacts with and stimulates DNA polymerase POLH.
Sequence similaritiesBelongs to the activator 1 small subunits family. CTF18 subfamily.
Cellular localizationNucleus. Associates with chromatin during S phase.
- Information by UniProt
- 6030457M03Rik antibody
- C16orf41 antibody
- C321D2.2 antibody
Anti-CTF18 antibody [2182C3a] (ab51331) at 1/100 dilution + HT1080 whole cell lysate at 25 µg
anti-mouse IgG at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 107 kDa
Observed band size: 118 kDa why is the actual band size different from the predicted?
Immunoprecipitation analysis using ab51331 at 5ug/500ug RIPA lysate of HeLa cells. Immunoblot using ab51331 at 1/100 dilution and anti-mouse IgG at 1/2500 dilution as secondary.
ab51331 has not yet been referenced specifically in any publications.