Overview

  • Product name

  • Description

    Rabbit polyclonal to CTF/NFIA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IHC-Fr, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow
  • Immunogen

    Recombinant fragment within Human CTF/NFIA (internal sequence). The exact sequence is proprietary.
    Database link: Q12857

  • Positive control

    • WB: HeLa nuclear lysate. IHC-P: Mouse liver and muscle tissues; Rat hindbrain tissue. IHC-Fr: Adult mouse retina tissue. ICC/IF: A431 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab228897 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 55 kDa.
IHC-P 1/100 - 1/1000.
IHC-Fr 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
  • Sequence similarities

    Belongs to the CTF/NF-I family.
    Contains 1 CTF/NF-I DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • 1110047K16Rik antibody
    • 9430022M17Rik antibody
    • CCAAT Box Binding Transcription Factor antibody
    • CCAAT-box-binding transcription factor antibody
    • CTF antibody
    • FLJ39164 antibody
    • NF I/A antibody
    • NF-I/A antibody
    • NF1-A antibody
    • NFI A antibody
    • NFI L antibody
    • NFI-A antibody
    • NFIA antibody
    • NFIA_HUMAN antibody
    • Nuclear factor 1 A type antibody
    • Nuclear factor 1 A-type antibody
    • Nuclear factor 1/A antibody
    • Nuclear factor I/A antibody
    • TGGCA binding protein antibody
    • TGGCA-binding protein antibody
    see all

Images

  • Anti-CTF/NFIA antibody (ab228897) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear lysate at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size: 55 kDa



    10% SDS-PAGE gel.

  • Frozen section of adult mouse retina tissue stained for CTF/NFIA using ab228897 at 1/250 dilution in immunohistochemical analysis.

    Nuclear counterstain: Fluoroshield with DAPI (blue). Beta-Tubulin is detected with an anti-Tubulin 3/TUJ1 antibody (red) at 1/250 dilution.

  • 4% paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells stained for CTF/NFIA (green) using ab228897 at 1/1000 dilution in ICC/IF.

    Nuclear counterstain: Hoechst 33342 (blue).

  • Paraffin-embedded mouse liver tissue stained for CTF/NFIA using ab228897 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded mouse muscle tissue stained for CTF/NFIA using ab228897 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded rat hindbrain tissue stained for CTF/NFIA using ab228897 at 1/500 dilution in immunohistochemical analysis.

References

ab228897 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228897.
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