Anti-CTGF antibody [17-73-F1] (ab94939)

Mouse monoclonal CTGF antibody [17-73-F1]. Validated in WB, ELISA and tested in Human. Cited in 1 publication(s). Independently reviewed in 1 review(s). Immunogen corresponding to synthetic peptide.

Overview

  • Product name

    Anti-CTGF antibody [17-73-F1]
    See all CTGF primary antibodies
  • Description

    Mouse monoclonal [17-73-F1] to CTGF
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Cow, Pig, Baboon
  • Immunogen

    Synthetic peptide corresponding to Human CTGF aa 150-250. corresponding to the hinge region of Human CTGF
    Database link: P29279

  • Positive control

    • This antibody gave a positive signal when tested against recombinant CTGF protein, and in the following lysates: HeLa whole cell; HEK293 whole cell; Bone marrow (human) tissue; ovary (human) tissue.
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    17-73-F1
  • Isotype

    IgG2
  • Light chain type

    lambda
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab94939 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 10 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
ELISA Use at an assay dependent dilution.

Target

  • Function

    Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis.
  • Tissue specificity

    Expressed in bone marrow and thymic cells. Also expressed one of two Wilms tumors tested.
  • Sequence similarities

    Belongs to the CCN family.
    Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 1 IGFBP N-terminal domain.
    Contains 1 TSP type-1 domain.
    Contains 1 VWFC domain.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix. Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • CCN 2 antibody
    • CCN family member 2 antibody
    • CCN2 antibody
    • Connective tissue growth factor antibody
    • Ctgf antibody
    • CTGF_HUMAN antibody
    • Hcs 24 antibody
    • Hcs24 antibody
    • Hypertrophic chondrocyte specific protein 24 antibody
    • Hypertrophic chondrocyte-specific gene product 24 antibody
    • Hypertrophic chondrocyte-specific protein 24 antibody
    • IBP-8 antibody
    • IGF-binding protein 8 antibody
    • IGFBP-8 antibody
    • IGFBP8 antibody
    • Insulin like Growth Factor Binding Protein 8 antibody
    • Insulin-like growth factor-binding protein 8 antibody
    • MGC102839 antibody
    • NOV 2 antibody
    • NOV2 antibody
    see all

Images

  • Anti-CTGF antibody [17-73-F1] (ab94939) at 10 µg/ml + CTGF Recombinant Protein (Human) at 0.1 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 38 kDa
    Observed band size: 38 kDa


    Exposure time: 4 minutes


    The human CTGF His-tagged fusion protein, produced in E. coli, is 38.3KDa protein containing 323 amino acid residues of the human CTGF and 23 additional amino acid residues- His Tag, Xa- cleavage site.
  • All lanes : Anti-CTGF antibody [17-73-F1] (ab94939) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
    Lane 4 : Human ovary tissue lysate - total protein (ab30222)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 38 kDa
    Observed band size: 38 kDa
    Additional bands at: 44 kDa (possible glycosylated form), 50 kDa, 60 kDa, 70 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 8 minutes


    CTGF contains two glycosylation sites (SwissProt) which may explain the higher migrating band.

References

This product has been referenced in:

See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Rabbit Tissue sections (Brain section)
Permeabilization
No
Specification
Brain section
Blocking step
NDS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde

Govindaiah Vinukonda

Verified customer

Submitted Jul 31 2018

Question
Answer


Le laboratoire vient de me confirmer que l'anticorps monoclonal de souris anti-GTCF, clone [17-73-F1], référence ab94939 est purifié par protéine G. La concentration est de 1 mg/ml.
La fiche technique a été mise à jour avec cette correction. Désolés pour cette erreur.
Le peptide immunogène correspond aux acides aminés 181-197 de la CTGF humaine.

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