Key features and details
- Rabbit polyclonal to CTGF
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-CTGF antibody
See all CTGF primary antibodies
DescriptionRabbit polyclonal to CTGF
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human CTGF aa 200-250 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: P29279
- WB: Mouse lung lysate. IHC-P: Human laryngeal cancer tissue; Rat heart tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol
Aqueous buffered solution
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab227180 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500.|
|WB||1/100 - 1/1000. Predicted molecular weight: 38 kDa.|
FunctionMajor connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis.
Tissue specificityExpressed in bone marrow and thymic cells. Also expressed one of two Wilms tumors tested.
Sequence similaritiesBelongs to the CCN family.
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 1 IGFBP N-terminal domain.
Contains 1 TSP type-1 domain.
Contains 1 VWFC domain.
Cellular localizationSecreted > extracellular space > extracellular matrix. Secreted.
- Information by UniProt
- CCN 2 antibody
- CCN family member 2 antibody
- CCN2 antibody
Anti-CTGF antibody (ab227180) at 1/300 dilution + Mouse lung lysate
Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/5000 dilution
Predicted band size: 38 kDa
Formalin-fixed, paraffin-embedded human laryngeal cancer tissue stained for CTFG using ab227180 at 1/200 dilution in immunohistochemical analysis, followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed, paraffin-embedded rat heart tissue stained for CTFG using ab227180 at 1/200 dilution in immunohistochemical analysis, followed by conjugation to the secondary antibody and DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227180 has been referenced in 2 publications.
- Song ZM et al. CTGF-mediated ERK signaling pathway influences the inflammatory factors and intestinal flora in ulcerative colitis. Biomed Pharmacother 111:1429-1437 (2019). PubMed: 30841458
- Weng Y et al. Effects of high glucose on proliferation and function of circulating fibrocytes: Involvement of CXCR4/SDF-1 axis. Int J Mol Med 44:927-938 (2019). PubMed: 31257476