Anti-CTGF antibody (ab5097)
Key features and details
- Rabbit polyclonal to CTGF
- Suitable for: ICC, IHC-Fr, WB, IHC-P, ICC/IF
- Reacts with: Rat, Human, Pig, Recombinant fragment
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-CTGF antibody
See all CTGF primary antibodies -
Description
Rabbit polyclonal to CTGF -
Host species
Rabbit -
Specificity
This antibody detects recombinant human CTGF by Western Blotting. The band can be blocked by the immunising peptide (ab13741). We have been unable to detect endogenous protein in WB with ab5097. -
Tested applications
Suitable for: ICC, IHC-Fr, WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human, Pig, Recombinant fragment -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5097 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use a concentration of 1 µg/ml.
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IHC-Fr | (2) |
Use at an assay dependent concentration.
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WB |
1/500. Predicted molecular weight: 38 kDa.
This application has only been tested on recombinant protein. |
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IHC-P | (6) |
Use at an assay dependent concentration. PubMed: 17028989
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ICC/IF | (1) |
Use at an assay dependent concentration.
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Notes |
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ICC
Use a concentration of 1 µg/ml. |
IHC-Fr
Use at an assay dependent concentration. |
WB
1/500. Predicted molecular weight: 38 kDa. This application has only been tested on recombinant protein. |
IHC-P
Use at an assay dependent concentration. PubMed: 17028989 |
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis. -
Tissue specificity
Expressed in bone marrow and thymic cells. Also expressed one of two Wilms tumors tested. -
Sequence similarities
Belongs to the CCN family.
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 1 IGFBP N-terminal domain.
Contains 1 TSP type-1 domain.
Contains 1 VWFC domain. -
Cellular localization
Secreted > extracellular space > extracellular matrix. Secreted. - Information by UniProt
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Database links
- Entrez Gene: 1490 Human
- Entrez Gene: 100152141 Pig
- Entrez Gene: 64032 Rat
- Omim: 121009 Human
- SwissProt: P29279 Human
- SwissProt: O19113 Pig
- SwissProt: Q9R1E9 Rat
- Unigene: 410037 Human
see all -
Alternative names
- CCN 2 antibody
- CCN family member 2 antibody
- CCN2 antibody
see all
Images
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ab5097 staining CTGF in HepG2 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab5097 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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Western blot, on recombinant Human CTGF produced in E. coli, using ab5097 at 1/500.
Not yet tested against endogenous CTGFSecondary antibody - anti-rabbit HRP (ab6721)
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ab5097 staining CTGF in Human Hela, cervix cancer cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 1% normal goat serum for 30 minutes. Samples were incubated with primary antibody (1/750) for 4 hours at 20°C. A Cy3®-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody (1/800).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (21)
ab5097 has been referenced in 21 publications.
- Bhat IP et al. Connective tissue growth factor expression hints at aggressive nature of colorectal cancer. World J Gastroenterol 28:547-569 (2022). PubMed: 35316957
- Jang SD et al. Anti-Cancer Activity Profiling of Chemotherapeutic Agents in 3D Co-Cultures of Pancreatic Tumor Spheroids with Cancer-Associated Fibroblasts and Macrophages. Cancers (Basel) 13:N/A (2021). PubMed: 34885065
- Verhagen JMA et al. Multi-Omics Profiling in Marfan Syndrome: Further Insights into the Molecular Mechanisms Involved in Aortic Disease. Int J Mol Sci 23:N/A (2021). PubMed: 35008861
- Wang Y et al. Connective tissue growth factor promotes retinal pigment epithelium mesenchymal transition via the PI3K/AKT signaling pathway. Mol Med Rep 23:N/A (2021). PubMed: 33760200
- Thankam FG et al. Biomarkers and heterogeneous fibroblast phenotype associated with incisional hernia. Mol Cell Biochem 476:3353-3363 (2021). PubMed: 33942219