Overview

  • Product name
  • Description
    Rabbit polyclonal to CTGF
  • Host species
    Rabbit
  • Specificity
    This antibody detects recombinant human CTGF by Western Blotting. The band can be blocked by the immunising peptide (ab13741). We have been unable to detect endogenous protein in WB with ab5097.
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Rat, Human, Pig
  • Immunogen

    Synthetic peptide corresponding to Human CTGF aa 150-250 conjugated to keyhole limpet haemocyanin.
    Database link: P29279
    (Peptide available as ab13741)

  • Positive control
    • This antibody gave a positive signal in recombinant human CTGF in WB; This antibody gave a positive signal in the following Formaldehyde fixed cell lines:HepG2.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS. pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 17028989
WB 1/500. Predicted molecular weight: 38 kDa.

This application has only been tested on recombinant protein.

ICC/IF Use a concentration of 5 µg/ml.
IHC-Fr 1/200.

Target

  • Function
    Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis.
  • Tissue specificity
    Expressed in bone marrow and thymic cells. Also expressed one of two Wilms tumors tested.
  • Sequence similarities
    Belongs to the CCN family.
    Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 1 IGFBP N-terminal domain.
    Contains 1 TSP type-1 domain.
    Contains 1 VWFC domain.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix. Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCN 2 antibody
    • CCN family member 2 antibody
    • CCN2 antibody
    • Connective tissue growth factor antibody
    • Ctgf antibody
    • CTGF_HUMAN antibody
    • Hcs 24 antibody
    • Hcs24 antibody
    • Hypertrophic chondrocyte specific protein 24 antibody
    • Hypertrophic chondrocyte-specific gene product 24 antibody
    • Hypertrophic chondrocyte-specific protein 24 antibody
    • IBP-8 antibody
    • IGF-binding protein 8 antibody
    • IGFBP-8 antibody
    • IGFBP8 antibody
    • Insulin like Growth Factor Binding Protein 8 antibody
    • Insulin-like growth factor-binding protein 8 antibody
    • MGC102839 antibody
    • NOV 2 antibody
    • NOV2 antibody
    see all

Images

  • Western blot, on recombinant Human CTGF produced in E. coli, using ab5097 at 1/500.

    Not yet tested against endogenous CTGF

     Secondary antibody - anti-rabbit HRP (ab6721)

  • ab5097 staining CTGF in human bronchus tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in formaldehyde and an antigen retrieval step was performed using EDTA pH9.0. Samples were then incubated with ab5097 at a 1/400 dilution for 20 minutes at 25°C. The secondary used was an undiluted HRP conjugated goat anti-mouse/rabbit polyclonal.

    See Abreview

  • ICC/IF image of ab5097 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab5097 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab5097 staining CTGF in human heart tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in buffer and blocking (5 minutes/peroxidase block then 10 minutes/protein block) for 15 minutes at 20°C. The primary antibody was diluted, 1/50 and incubated with sample for 45 minutes at 20°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary.

    See Abreview

  • ab5097 staining CTGF in Human Hela, cervix cancer cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 1% normal goat serum for 30 minutes. Samples were incubated with primary antibody (1/750) for 4 hours at 20°C. A Cy3®-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody (1/800).

    See Abreview

  • ab5097 staining CTGF in Pig skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 2 hours at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200 in blocking buffer) for 24 hours at 4°C. A BHRP-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • ab5097 staining CTGF in Pig skin tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 5% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/200 in PBS Tween 0.01% + donkey serum 1%) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Donkey anti-rat IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Sourbier C  et al. Targeting loss of the Hippo signaling pathway in NF2-deficient papillary kidney cancers. Oncotarget 9:10723-10733 (2018). Read more (PubMed: 29535838) »
  • Lambers E  et al. Foxc1 Regulates Early Cardiomyogenesis and Functional Properties of Embryonic Stem Cell Derived Cardiomyocytes. Stem Cells 34:1487-500 (2016). Read more (PubMed: 26824887) »
See all 13 Publications for this product

Customer reviews and Q&As

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1-8 of 8 Abreviews

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Specification
skin
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jun 10 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Pig Tissue sections (Ureter)
Specification
Ureter
Fixative
Paraformaldehyde
Permeabilization
Yes - 0,2 % Trition/PBS
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1%

Ms. Beate Thode

Verified customer

Submitted Feb 22 2013

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Hela, Cervix Cancer Cells)
Specification
Hela, Cervix Cancer Cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0,2 Triton/PBS
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1%

Ms. Beate Thode

Verified customer

Submitted Feb 22 2013

Application
Immunohistochemistry (Frozen sections)
Sample
Pig Tissue sections (skin)
Specification
skin
Fixative
Formaldehyde
Permeabilization
Yes - Triton 0.1% (5min)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Gabrielle Le Provost

Verified customer

Submitted Jun 01 2012

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (bronchus)
Specification
bronchus
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA, pH9.0, 20 min, 100C
Permeabilization
No

Abcam user community

Verified customer

Submitted Nov 02 2011

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Skin)
Specification
Skin
Fixative
10% NBF
Antigen retrieval step
None
Blocking step
Serum as blocking agent for 5 minute(s) · Concentration: .25% · Temperature: 25°C

Ms. Elizabeth Chlipala

Verified customer

Submitted Aug 02 2010

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (lung (pulmonary fibrosis))
Specification
lung (pulmonary fibrosis)
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate-Buffer
Permeabilization
No
Blocking step
SuperBlock as blocking agent for 20 minute(s) · Concentration: 100%

Mr. Nico Jentsch

Verified customer

Submitted Sep 28 2009

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (heart)
Specification
heart
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
Permeabilization
No
Blocking step
5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C

Mr. Antibody Solutions

Verified customer

Submitted Apr 02 2009

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