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    ctgf-antibody-ab6992.pdf

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Cardiovascular Angiogenesis Growth Factors Other
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Anti-CTGF antibody (ab6992)

  • Datasheet
  • SDS
Reviews (27)Q&A (34)References (214)

Product price, shipping and contact information

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Key features and details

  • Rabbit polyclonal to CTGF
  • Reacts with: Mouse, Rat, Sheep, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-CTGF antibody
    See all CTGF primary antibodies
  • Description

    Rabbit polyclonal to CTGF
  • Host species

    Rabbit
  • Species reactivity

    Reacts with: Mouse, Rat, Sheep, Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment corresponding to Mouse CTGF aa 223-348.
    Database link: P29268
    (Peptide available as ab7861)

  • Positive control

    • human hippocampal protein extracts (see Ueberham et al paper).
  • General notes

      

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Growth Factors
    • Other
    • Immunology
    • Secreted Molecules
    • CCN Family

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human CTGF protein (His tag) (ab203539)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)

Target

  • Function

    Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis.
  • Tissue specificity

    Expressed in bone marrow and thymic cells. Also expressed one of two Wilms tumors tested.
  • Sequence similarities

    Belongs to the CCN family.
    Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 1 IGFBP N-terminal domain.
    Contains 1 TSP type-1 domain.
    Contains 1 VWFC domain.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix. Secreted.
  • Target information above from: UniProt accession P29279 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 281103 Cow
    • Entrez Gene: 1490 Human
    • Entrez Gene: 14219 Mouse
    • Entrez Gene: 64032 Rat
    • Omim: 121009 Human
    • SwissProt: O18739 Cow
    • SwissProt: P29279 Human
    • SwissProt: P29268 Mouse
    • SwissProt: Q9R1E9 Rat
    • Unigene: 410037 Human
    • Unigene: 390287 Mouse
    • Unigene: 17145 Rat
    see all
  • Alternative names

    • CCN 2 antibody
    • CCN family member 2 antibody
    • CCN2 antibody
    • Connective tissue growth factor antibody
    • Ctgf antibody
    • CTGF_HUMAN antibody
    • Hcs 24 antibody
    • Hcs24 antibody
    • Hypertrophic chondrocyte specific protein 24 antibody
    • Hypertrophic chondrocyte-specific gene product 24 antibody
    • Hypertrophic chondrocyte-specific protein 24 antibody
    • IBP-8 antibody
    • IGF-binding protein 8 antibody
    • IGFBP-8 antibody
    • IGFBP8 antibody
    • Insulin like Growth Factor Binding Protein 8 antibody
    • Insulin-like growth factor-binding protein 8 antibody
    • MGC102839 antibody
    • NOV 2 antibody
    • NOV2 antibody
    see all

Protocols

  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (214)

    Publishing research using ab6992? Please let us know so that we can cite the reference in this datasheet.

    ab6992 has been referenced in 214 publications.

    • Wilson DH  et al. Non-canonical Wnt signalling regulates scarring in biliary disease via the planar cell polarity receptors. Nat Commun 11:445 (2020). PubMed: 31974352
    • Cao Y  et al. BAFF is involved in the pathogenesis of IgA nephropathy by activating the TRAF6/NF-?B signaling pathway in glomerular mesangial cells. Mol Med Rep 21:795-805 (2020). PubMed: 31974601
    • Yu Q  et al. Antifibrotic effects of specific siRNA targeting connective tissue growth factor delivered by polyethyleneimine-functionalized magnetic iron oxide nanoparticles on LX-2 cells. Mol Med Rep 21:181-190 (2020). PubMed: 31746398
    • Fallah M  et al. Plasminogen is a master regulator and a potential drug candidate for the healing of radiation wounds. Cell Death Dis 11:201 (2020). PubMed: 32205839
    • Omori H  et al. YAP1 is a potent driver of the onset and progression of oral squamous cell carcinoma. Sci Adv 6:eaay3324 (2020). PubMed: 32206709
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-10 of 61 Abreviews or Q&A

    Question

    IHC-P with mouse skin no signal tried with a nd withour AR (citrate buffer, microwave) warm vial (was left out for 2 days on loading dock) used same Ab before and worked, different lot Ab: 1/100 - 1/1000 several dilutions, o/n 4 degree block with goat serum 10% + TX-100 secondary: fine

    Read More

    Abcam community

    Verified customer

    Asked on Nov 29 2011

    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

    Read More

    Abcam Scientific Support

    Answered on Nov 29 2011

    Western blot abreview for Anti-CTGF antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (lung)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (10% gel)
    Loading amount
    30 µg
    Treatment
    particles
    Specification
    lung
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Pureunhaneul Lee

    Verified customer

    Submitted Dec 25 2019

    Western blot abreview for Anti-CTGF antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (mouse neural stem cell)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    50 µg
    Specification
    mouse neural stem cell
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 15°C
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 17 2019

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-CTGF antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (brain)
    Antigen retrieval step
    None
    Permeabilization
    No
    Specification
    brain
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 19 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CTGF antibody

    Poor
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Prostate)
    Antigen retrieval step
    None
    Permeabilization
    No
    Specification
    Prostate
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 02 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CTGF antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rhesus monkey Tissue sections (Jejunum)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Ventana CC1 (Tris/EDTA)
    Permeabilization
    No
    Specification
    Jejunum
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 19 2017

    Western blot abreview for Anti-CTGF antibody

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Human Cell lysate - whole cell (iPSC derived nuerons)
    Specification
    iPSC derived nuerons
    Blocking step
    Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 26 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CTGF antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 15% · Temperature: 20°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate
    Sample
    Human Tissue sections (saphenous vein)
    Specification
    saphenous vein
    Permeabilization
    Yes - tween20
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 19 2014

    Western blot abreview for Anti-CTGF antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Human Cell lysate - whole cell (retina)
    Specification
    retina
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 12 2014

    Western blot abreview for Anti-CTGF antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (12% Bis-Tris)
    Sample
    Human Tissue lysate - whole (Skeletal Muscle)
    Specification
    Skeletal Muscle
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 03 2014

    1-10 of 61 Abreviews or Q&A

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