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  1. Link

    ctgf-antibody-ab6992.pdf

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Cardiovascular Angiogenesis Growth Factors Other
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Anti-CTGF antibody (ab6992)

  • Datasheet
  • SDS
Reviews (27)Q&A (34)References (262)

Product price, shipping and contact information

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Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Key features and details

  • Rabbit polyclonal to CTGF
  • Suitable for: IHC-P, ICC/IF, IHC-FoFr, WB, IHC-Fr, IP
  • Reacts with: Mouse, Rat, Sheep, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-CTGF antibody [EPR20728] (ab209780)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-CTGF antibody
    See all CTGF primary antibodies
  • Description

    Rabbit polyclonal to CTGF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, IHC-FoFr, WB, IHC-Fr, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Sheep, Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment corresponding to Mouse CTGF aa 223-348.
    Database link: P29268
    (Peptide available as ab7861)

  • Positive control

    • human hippocampal protein extracts (see Ueberham et al paper).
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Growth Factors
    • Other
    • Immunology
    • Secreted Molecules
    • CCN Family

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab6992 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (10)
Use at an assay dependent concentration.
ICC/IF (4)
Use at an assay dependent concentration.
IHC-FoFr (1)
Use at an assay dependent concentration.
WB (10)
Use at an assay dependent concentration. Predicted molecular weight: 38 kDa.

Block membrane in 4% BSA in Tris-buffered saline containing 0.05% Tween 20 (TBST). See Fu M et al.

IHC-Fr (2)
Use at an assay dependent concentration.

See Ueberham et al.

IP
Use at an assay dependent concentration. PubMed: 16100286
Notes
IHC-P
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IHC-FoFr
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 38 kDa.

Block membrane in 4% BSA in Tris-buffered saline containing 0.05% Tween 20 (TBST). See Fu M et al.

IHC-Fr
Use at an assay dependent concentration.

See Ueberham et al.

IP
Use at an assay dependent concentration. PubMed: 16100286

Target

  • Function

    Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis.
  • Tissue specificity

    Expressed in bone marrow and thymic cells. Also expressed one of two Wilms tumors tested.
  • Sequence similarities

    Belongs to the CCN family.
    Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 1 IGFBP N-terminal domain.
    Contains 1 TSP type-1 domain.
    Contains 1 VWFC domain.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix. Secreted.
  • Target information above from: UniProt accession P29279 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 281103 Cow
    • Entrez Gene: 1490 Human
    • Entrez Gene: 14219 Mouse
    • Entrez Gene: 64032 Rat
    • Omim: 121009 Human
    • SwissProt: O18739 Cow
    • SwissProt: P29279 Human
    • SwissProt: P29268 Mouse
    • SwissProt: Q9R1E9 Rat
    • Unigene: 410037 Human
    • Unigene: 390287 Mouse
    • Unigene: 17145 Rat
    see all
  • Alternative names

    • CCN 2 antibody
    • CCN family member 2 antibody
    • CCN2 antibody
    • Connective tissue growth factor antibody
    • Ctgf antibody
    • CTGF_HUMAN antibody
    • Hcs 24 antibody
    • Hcs24 antibody
    • Hypertrophic chondrocyte specific protein 24 antibody
    • Hypertrophic chondrocyte-specific gene product 24 antibody
    • Hypertrophic chondrocyte-specific protein 24 antibody
    • IBP-8 antibody
    • IGF-binding protein 8 antibody
    • IGFBP-8 antibody
    • IGFBP8 antibody
    • Insulin like Growth Factor Binding Protein 8 antibody
    • Insulin-like growth factor-binding protein 8 antibody
    • MGC102839 antibody
    • NOV 2 antibody
    • NOV2 antibody
    see all

Protocols

  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (262)

Publishing research using ab6992? Please let us know so that we can cite the reference in this datasheet.

ab6992 has been referenced in 262 publications.

  • Zhang Y  et al. miR-212 promotes renal interstitial fibrosis by inhibiting hypoxia-inducible factor 1-a inhibitor. Mol Med Rep 23:N/A (2021). PubMed: 33495813
  • Lyu L  et al. Scoparone alleviates Ang II-induced pathological myocardial hypertrophy in mice by inhibiting oxidative stress. J Cell Mol Med 25:3136-3148 (2021). PubMed: 33560596
  • Zhu F  et al. Tubular Numb promotes renal interstitial fibrosis via modulating HIF-1a protein stability. Biochim Biophys Acta Mol Basis Dis 1867:166081 (2021). PubMed: 33486098
  • Tokuda K  et al. Development of a novel knee contracture mouse model by immobilization using external fixation. Connect Tissue Res N/A:1-14 (2021). PubMed: 33602048
  • Liu Y  et al. Inhibiting miR-1 attenuates pulmonary arterial hypertension in rats. Mol Med Rep 23:N/A (2021). PubMed: 33604679
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-10 of 61 Abreviews or Q&A

Western blot abreview for Anti-CTGF antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (lung)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10% gel)
Loading amount
30 µg
Treatment
particles
Specification
lung
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Pureunhaneul Lee

Verified customer

Submitted Dec 25 2019

Western blot abreview for Anti-CTGF antibody

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse neural stem cell)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
50 µg
Specification
mouse neural stem cell
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 15°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Sep 17 2019

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-CTGF antibody

Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (brain)
Antigen retrieval step
None
Permeabilization
No
Specification
brain
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 19 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CTGF antibody

Poor
Abreviews
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Prostate)
Antigen retrieval step
None
Permeabilization
No
Specification
Prostate
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 02 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CTGF antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rhesus monkey Tissue sections (Jejunum)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Ventana CC1 (Tris/EDTA)
Permeabilization
No
Specification
Jejunum
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 19 2017

Western blot abreview for Anti-CTGF antibody

Good
Abreviews
Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (iPSC derived nuerons)
Specification
iPSC derived nuerons
Blocking step
Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 26 2015

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CTGF antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 15% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Sample
Human Tissue sections (saphenous vein)
Specification
saphenous vein
Permeabilization
Yes - tween20
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 19 2014

Western blot abreview for Anti-CTGF antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (retina)
Specification
retina
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 12 2014

Western blot abreview for Anti-CTGF antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (12% Bis-Tris)
Sample
Human Tissue lysate - whole (Skeletal Muscle)
Specification
Skeletal Muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 03 2014

Question

IHC-P with mouse skin no signal tried with a nd withour AR (citrate buffer, microwave) warm vial (was left out for 2 days on loading dock) used same Ab before and worked, different lot Ab: 1/100 - 1/1000 several dilutions, o/n 4 degree block with goat serum 10% + TX-100 secondary: fine

Read More

Abcam community

Verified customer

Asked on Nov 29 2011

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

Read More

Abcam Scientific Support

Answered on Nov 29 2011

1-10 of 61 Abreviews or Q&A

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