Anti-CTGF antibody - C-terminal (ab238246)
Key features and details
- Goat polyclonal to CTGF - C-terminal
- Suitable for: ICC/IF
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-CTGF antibody - C-terminal
See all CTGF primary antibodies -
Description
Goat polyclonal to CTGF - C-terminal -
Host species
Goat -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Cow, Human, Pig -
Immunogen
Synthetic peptide corresponding to Human CTGF aa 300 to the C-terminus (C terminal) (Cysteine residue). (NP_001892).
Database link: P29279-1 -
Positive control
- ICC/IF: NIH/3T3 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab238246 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 5 µg/ml.
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Notes |
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ICC/IF
Use a concentration of 5 µg/ml. |
Target
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Function
Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis. -
Tissue specificity
Expressed in bone marrow and thymic cells. Also expressed one of two Wilms tumors tested. -
Sequence similarities
Belongs to the CCN family.
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 1 IGFBP N-terminal domain.
Contains 1 TSP type-1 domain.
Contains 1 VWFC domain. -
Cellular localization
Secreted > extracellular space > extracellular matrix. Secreted. - Information by UniProt
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Database links
- Entrez Gene: 281103 Cow
- Entrez Gene: 1490 Human
- Entrez Gene: 14219 Mouse
- Entrez Gene: 100152141 Pig
- Entrez Gene: 64032 Rat
- Omim: 121009 Human
- SwissProt: O18739 Cow
- SwissProt: P29279 Human
see all -
Alternative names
- CCN 2 antibody
- CCN family member 2 antibody
- CCN2 antibody
see all
Images
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Immunofluorescent analysis of paraformaldehyde-fixed NIH/3T3 (mouse embryo fibroblast cell line) cells, permeabilized with 0.15% Triton, labeling CTGF with ab238246 at 5 µg/ml. Primary incubation time 1 hour followed by an Alexa-Fluor®488 secondary antibody (2 µg/ml). The nuclear stain is DAPI (blue).
Negative control: Non-immunized goat IgG (5 µg/ml) followed by an Alexa-Fluor®488 secondary antibody (2 µg/ml).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab238246 has not yet been referenced specifically in any publications.