Key features and details
- Goat polyclonal to CTGF - C-terminal
- Suitable for: ICC/IF
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-CTGF antibody - C-terminal
See all CTGF primary antibodies
DescriptionGoat polyclonal to CTGF - C-terminal
Tested applicationsSuitable for: ICC/IFmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat, Cow, Human, Pig
- ICC/IF: NIH/3T3 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab238246 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionMajor connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis.
Tissue specificityExpressed in bone marrow and thymic cells. Also expressed one of two Wilms tumors tested.
Sequence similaritiesBelongs to the CCN family.
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 1 IGFBP N-terminal domain.
Contains 1 TSP type-1 domain.
Contains 1 VWFC domain.
Cellular localizationSecreted > extracellular space > extracellular matrix. Secreted.
- Information by UniProt
- CCN 2 antibody
- CCN family member 2 antibody
- CCN2 antibody
Immunofluorescent analysis of paraformaldehyde-fixed NIH/3T3 (mouse embryo fibroblast cell line) cells, permeabilized with 0.15% Triton, labeling CTGF with ab238246 at 5 µg/ml. Primary incubation time 1 hour followed by an Alexa-Fluor®488 secondary antibody (2 µg/ml). The nuclear stain is DAPI (blue).
Negative control: Non-immunized goat IgG (5 µg/ml) followed by an Alexa-Fluor®488 secondary antibody (2 µg/ml).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab238246 has not yet been referenced specifically in any publications.