Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20728] to CTGF
- Suitable for: WB, IP
- Reacts with: Mouse, Human
Product nameAnti-CTGF antibody [EPR20728]
See all CTGF primary antibodies
DescriptionRabbit monoclonal [EPR20728] to CTGF
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment within Human CTGF aa 1-300. The exact sequence is proprietary.
Database link: P29279
- WB: Starved NIH/3T3 cells treated with TGF-ß1 (ab50036) and Heparin sodium salt and HepG2 whole cell lysate. IP: HepG2 whole cell lysate.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab209780 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 38 kDa.|
FunctionMajor connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis.
Tissue specificityExpressed in bone marrow and thymic cells. Also expressed one of two Wilms tumors tested.
Sequence similaritiesBelongs to the CCN family.
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 1 IGFBP N-terminal domain.
Contains 1 TSP type-1 domain.
Contains 1 VWFC domain.
Cellular localizationSecreted > extracellular space > extracellular matrix. Secreted.
- Information by UniProt
- CCN 2 antibody
- CCN family member 2 antibody
- CCN2 antibody
CTGF was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate with ab209780 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab209780 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate 10 µg (Input).
Lane 2: ab209780 IP in HepG2 whole cell lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab209780 in HepG2 whole cell lysate (-).
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
The molecular mass observed is consistent with the literature. (PMID:27126736)
All lanes : Anti-CTGF antibody [EPR20728] (ab209780) at 1/1000 dilution
Lane 1 : NIH/3T3 (mouse embryonic fibroblast) starved for 18 hours, whole cell lysate
Lane 2 : NIH/3T3 starved for 18 hours, then treated with 10 ng/ml transforming growth factor-ß (TGF-ß1, ab50036) and 50 µg/ml Heparin sodium salt for 24 hours, whole cell lysate
Lane 3 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 38 kDa
Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
The level of CTGF expression can be induced by TGF beta treatment (PMID: 17786299).
CTGF is constitutively expressed in HepG2 cells (PMID:15886528).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab209780 has not yet been referenced specifically in any publications.