Anti-CTHRC1 antibody (ab264410)
Key features and details
- Rabbit polyclonal to CTHRC1
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CTHRC1 antibody
See all CTHRC1 primary antibodies -
Description
Rabbit polyclonal to CTHRC1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human CTHRC1 aa 25-75. The exact sequence is proprietary.
Database link: Q96CG8 -
Positive control
- WB: UACC-62 whole cell lysate. IP: UACC-62 whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab264410 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IP |
Use at an assay dependent concentration.
Use 50-100 µl/mg lysate. |
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WB |
1/1000. Predicted molecular weight: 26 kDa.
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Notes |
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IP
Use at an assay dependent concentration. Use 50-100 µl/mg lysate. |
WB
1/1000. Predicted molecular weight: 26 kDa. |
Target
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Function
May act as a negative regulator of collagen matrix deposition. -
Tissue specificity
Isoform 1 is expressed in calcified atherosclerotic plaque and chondrocyte-like cells. -
Sequence similarities
Contains 1 collagen-like domain. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 115908 Human
- Omim: 610635 Human
- SwissProt: Q96CG8 Human
- Unigene: 405614 Human
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Alternative names
- Collagen triple helix repeat containing 1 antibody
- Collagen triple helix repeat containing protein 1 antibody
- Collagen triple helix repeat-containing protein 1 antibody
see all
Images
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All lanes : Anti-CTHRC1 antibody (ab264410) at 1/1000 dilution
Lane 1 : UACC-62 whole cell lysate
Lane 2 : MALME-3M whole cell lysate
Lane 3 : SR whole cell lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 26 kDa
Exposure time: 10 secondsLysates prepared using NETN lysis buffer.
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CTHRC1 was immunoprecipitated from UACC-62 whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) with ab264410 at 100 µl per reaction. Western blot was performed from the immunoprecipitate using an alternative anti-CTHRC1 antibody
Lane 1: ab264410 IP in UACC-62 whole cell lysate.
Lane 2: Control IgG IP in UACC-62 whole cell lysate.Detection: Chemiluminescence with an exposure time of 1 second.
Lysates prepared using NETN lysis buffer.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab264410 has been referenced in 1 publication.
- Chen L et al. NEDD4L-induced β-catenin ubiquitination suppresses the formation and progression of interstitial pulmonary fibrosis via inhibiting the CTHRC1/HIF-1α axis. Int J Biol Sci 17:3320-3330 (2021). PubMed: 34512149