Anti-CTHRC1 antibody (ab85739)
Key features and details
- Rabbit polyclonal to CTHRC1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CTHRC1 antibody
See all CTHRC1 primary antibodies -
Description
Rabbit polyclonal to CTHRC1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow
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Immunogen
Synthetic peptide corresponding to Human CTHRC1 aa 150 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab101727) -
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab85739 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 26 kDa). | |
| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | Use a concentration of 5 µg/ml. |
Target
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Function
May act as a negative regulator of collagen matrix deposition. -
Tissue specificity
Isoform 1 is expressed in calcified atherosclerotic plaque and chondrocyte-like cells. -
Sequence similarities
Contains 1 collagen-like domain. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 115908 Human
- Entrez Gene: 68588 Mouse
- Entrez Gene: 282836 Rat
- Omim: 610635 Human
- SwissProt: Q96CG8 Human
- SwissProt: Q9D1D6 Mouse
- SwissProt: Q8CG08 Rat
- Unigene: 405614 Human
see all -
Alternative names
- Collagen triple helix repeat containing 1 antibody
- Collagen triple helix repeat containing protein 1 antibody
- Collagen triple helix repeat-containing protein 1 antibody
see all
Images
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Western blot - Anti-CTHRC1 antibody (ab85739)All lanes : Anti-CTHRC1 antibody (ab85739) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human heart tissue lysate - total protein (ab29431)
Lane 3 : Human blood vessel: artery normal tissue lysate - membrane extract (ab28989)
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
Exposure time: 10 minutes
We predict that the 20 kDa band represents the cleaved form of CTHRC1. Abcam welcomes customer feedback. -
Immunocytochemistry/ Immunofluorescence - Anti-CTHRC1 antibody (ab85739)ICC/IF image of ab85739 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85739, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Western blot - Anti-CTHRC1 antibody (ab85739)All lanes : Anti-CTHRC1 antibody (ab85739) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human heart tissue lysate - total protein (ab29431)
Lane 3 : Human blood vessel: artery normal tissue lysate - membrane extract (ab28989)
Lane 4 : Human brain tissue lysate - total protein (ab29466) with Human CTHRC1 peptide (ab101727) at 1 µg/ml
Lane 5 : Human heart tissue lysate - total protein (ab29431) with Human CTHRC1 peptide (ab101727) at 1 µg/ml
Lane 6 : Human blood vessel: artery normal tissue lysate - membrane extract (ab28989) with Human CTHRC1 peptide (ab101727) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTHRC1 antibody (ab85739)IHC image of CTHRC1 staining in human skin melanoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85739, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
References (10)
ab85739 has been referenced in 10 publications.
- Tsukui T et al. Collagen-producing lung cell atlas identifies multiple subsets with distinct localization and relevance to fibrosis. Nat Commun 11:1920 (2020). PubMed: 32317643
- Yin H et al. Identification of a 13-mRNA signature for predicting disease progression and prognosis in patients with bladder cancer. Oncol Rep 43:379-394 (2020). PubMed: 31894276
- Zheng M et al. CTHRC1 overexpression promotes cervical carcinoma progression by activating the Wnt/PCP signaling pathway. Oncol Rep 41:1531-1538 (2019). PubMed: 30628714
- Ni S et al. CTHRC1 overexpression predicts poor survival and enhances epithelial-mesenchymal transition in colorectal cancer. Cancer Med 7:5643-5654 (2018). PubMed: 30302922
- Wang CY et al. MicroRNA-98 suppresses cell proliferation, migration and invasion by targeting collagen triple helix repeat containing 1 in hepatocellular carcinoma. Mol Med Rep 13:2639-44 (2016). PubMed: 26846175
- Qi C et al. Preliminary observations regarding the expression of collagen triple helix repeat-containing 1 is an independent prognostic factor for Wilms' tumor. J Pediatr Surg 51:1501-6 (2016). WB, IHC-P ; Human . PubMed: 27230801
- Eriksson J et al. Gene expression analyses of primary melanomas reveal CTHRC1 as an important player in melanoma progression. Oncotarget 7:15065-92 (2016). WB . PubMed: 26918341
- Lee CE et al. Collagen Triple Helix Repeat Containing-1 (CTHRC1) Expression in Oral Squamous Cell Carcinoma (OSCC): Prognostic Value and Clinico-Pathological Implications. Int J Med Sci 12:937-45 (2015). IHC . PubMed: 26664254
- Ke Z et al. Overexpression of collagen triple helix repeat containing 1 (CTHRC1) is associated with tumour aggressiveness and poor prognosis in human non-small cell lung cancer. Oncotarget 5:9410-24 (2014). PubMed: 25238260
- Kim HC et al. Collagen triple helix repeat containing 1 (CTHRC1) acts via ERK-dependent induction of MMP9 to promote invasion of colorectal cancer cells. Oncotarget 5:519-29 (2014). IHC . PubMed: 24504172
