Immunohistochemistry (Frozen sections) abreview for Anti-CTHRC1 antibody

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Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (E8.5 mouse embryo sections)
Specification
E8.5 mouse embryo sections
Fixative
Paraformaldehyde
Permeabilization
Yes - PBST (PBS/0.5%v/v TritonX100 (Sigma)
Blocking step
NOVOCASTRA protein block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C

Other product details

Dilution
1/100
Incubation time
48 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA + 5% donkey serum + PBST

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Fluor 555 donkey anti rabbit (Invitrogen)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Dilution
1/1000

Additional data

Additional Notes
According to current literature, Cthrc1 expresses in notochord at E8.5 mouse embryo.

Here is a transverse section of E8.5 embryo.

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Submitted Jun 08 2012

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