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Immunohistochemistry (Frozen sections)
Mouse Tissue sections (E8.5 mouse embryo sections)
E8.5 mouse embryo sections
Yes - PBST (PBS/0.5%v/v TritonX100 (Sigma)
NOVOCASTRA protein block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Other product details
48 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA + 5% donkey serum + PBST
Non-Abcam antibody was used: Alexa Fluor 555 donkey anti rabbit (Invitrogen)
Host species: Donkey
Conjugation: Alexa Fluor® 555
According to current literature, Cthrc1 expresses in notochord at E8.5 mouse embryo.
Here is a transverse section of E8.5 embryo.
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Submitted Jun 08 2012