• Product name

  • Description

    Rabbit polyclonal to CtIP
  • Host species

  • Tested applications

    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human CtIP (internal sequence) conjugated to keyhole limpet haemocyanin.
    Database link: Q99708

  • Positive control

    • Human Breast, Colon Myenteric Plexus and Pancreas tissues.



Our Abpromise guarantee covers the use of ab117722 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 101 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • Function

    Endonuclease that cooperates with the MRE11-RAD50-NBN (MRN) complex in processing meiotic and mitotic double-strand breaks (DSBs) by ensuring both resection and intrachromosomal association of the broken ends. Functions downstream of the MRN complex and ATM, promotes ATR activation and its recruitment to DSBs in the S/G2 phase facilitating the generation of ssDNA. Component of the BRCA1-RBBP8 complex that regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage. Promotes microhomology-mediated alternative end joining (A-NHEJ) during class-switch recombination and plays an essential role in chromosomal translocations.
  • Involvement in disease

    Seckel syndrome 2
    Jawad syndrome
    Genetic variability in RBBP8 is noted as a factor in BRCA1-associated breast cancer risk (PubMed:21799032). Exhibits sensitivity to tamoxifen in certain breast cancer cell lines (PubMed:18171986).
  • Sequence similarities

    Belongs to the COM1/SAE2/CtIP family.
  • Domain

    The PXDLS motif binds to a cleft in CtBP proteins.
    The damage-recruitment motif is required for DNA binding and translocation to sites of DNA damage.
  • Post-translational

    Acetylated. Deacetylation by SIRT6 upon DNA damage promotes DNA end resection.
    Hyperphosphorylation upon ionizing radiation results in dissociation from BRCA1. Phosphorylation at Thr-847 by CDK1 is essential for the recruitment to DNA and the DNA repair function. Phosphorylated on Ser-327 as cells enter G2 phase. This phosphorylation is required for binding BRCA1 and for the G2/M DNA damage transition checkpoint control.
    Ubiquitinated (PubMed:14654780, PubMed:16818604). Ubiquitination at multiple sites by BRCA1 (via its N-terminal RING domain) does not lead to its proteosomal degradation but instead the ubiquitinated RBBP8 binds to chromatin following DNA damage and may play a role in G2/M checkpoint control (PubMed:16818604). Ubiquitinated by RNF138 at its N-terminus (PubMed:26502057).
  • Cellular localization

    Nucleus. Chromosome. Associates with sites of DNA damage in S/G2 phase (PubMed:10764811). Ubiquitinated RBBP8 binds to chromatin following DNA damage (PubMed:16818604).
  • Information by UniProt
  • Database links

  • Alternative names

    • COM1 antibody
    • COM1_HUMAN antibody
    • CtBP interacting protein antibody
    • CtBP-interacting protein antibody
    • CtIP antibody
    • DNA endonuclease RBBP8 antibody
    • JWDS antibody
    • RB binding protein 8 endonuclease antibody
    • RBBP-8 antibody
    • RBBP8 antibody
    • Retinoblastoma-binding protein 8 antibody
    • Retinoblastoma-interacting protein and myosin-like antibody
    • Rim antibody
    • SAE2 antibody
    • SCKL2 antibody
    • Sporulation in the absence of SPO11 protein 2 homolog antibody
    see all


  • Lane 1 : Anti-CtIP antibody (ab117722) at 1 µg/ml
    Lane 2 : Anti-CtIP antibody (ab117722) at 2 µg/ml

    Lane 1 : Mouse spleen tissue lystae
    Lane 2 : Mouse spleen tissue lysate

    Predicted band size: 101 kDa

  • Immunohistochemistry Image of Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE) stained with anti-CTIP antibody (ab117722).
  • Immunohistochemistry Image of Human Colon, Myenteric Plexus: Formalin-Fixed, Paraffin-Embedded (FFPE) stained with anti-CTIP antibody (ab117722).
  • Immunohistochemistry Image of Human Pancreas, Duct: Formalin-Fixed, Paraffin-Embedded (FFPE) stained with anti-CTIP antibody (ab117722).


ab117722 has not yet been referenced specifically in any publications.

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