Anti-Ctip1/BCL-11A antibody (ab264122)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Ctip1/BCL-11A antibody
See all Ctip1/BCL-11A primary antibodies -
Description
Rabbit polyclonal to Ctip1/BCL-11A -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide aa 450-500. The exact sequence is proprietary.
Database link: Q9H165 -
Positive control
- WB: Ramos and HEK-293T whole cell lysate. IP: Ramos whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab264122 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at 2-10 µg/mg of lysate. | |
| WB | 1/2000 - 1/10000. |
Target
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Function
Functions as a myeloid and B-cell proto-oncogene. May play important roles in leukemogenesis and hematopoiesis. An essential factor in lymphopoiesis, is required for B-cell formation in fetal liver. May function as a modulator of the transcriptional repression activity of ARP1. -
Tissue specificity
Expressed at high levels in brain, spleen thymus, bone marrow and testis. Expressed in CD34-positive myeloid precursor cells, B-cells, monocytes and megakaryocytes. Expression is tightly regulated during B-cell development. -
Involvement in disease
Note=Chromosomal aberrations involving BCL11A may be a cause of lymphoid malignancies. Translocation t(2;14)(p13;q32.3) causes BCL11A deregulation and amplification. -
Sequence similarities
Contains 6 C2H2-type zinc fingers. -
Post-translational
modificationsSumoylated by SUMO1. -
Cellular localization
Cytoplasm. Nucleus. Associates with the nuclear body. Colocalizes with SUMO1 and SENP2 in nuclear speckles. - Information by UniProt
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Database links
- Entrez Gene: 53335 Human
- Entrez Gene: 14025 Mouse
- Omim: 606557 Human
- SwissProt: Q9H165 Human
- SwissProt: Q9QYE3 Mouse
- Unigene: 370549 Human
- Unigene: 441102 Mouse
- Unigene: 471050 Mouse
see all -
Alternative names
- 2810047E18Rik antibody
- B cell CLL/lymphoma 11A (zinc finger protein) antibody
- B cell CLL/lymphoma 11A (zinc finger protein) isoform 2 antibody
see all
Images
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Western blot - Anti-Ctip1/BCL-11A antibody (ab264122)All lanes : Anti-Ctip1/BCL-11A antibody (ab264122) at 0.1 µg/ml
Lane 1 : Ramos whole cell lysate at 50 µg
Lane 2 : Ramos whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : HEK-293T whole cell lysate at 50 µg
Developed using the ECL technique.
Exposure time: 3 minutes4-8% SDS-PAGE.
Cells prepared using NETN lysis buffer.
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Immunoprecipitation - Anti-Ctip1/BCL-11A antibody (ab264122)Ctip1/BCL-11A was immunoprecipitated from 1mg Ramos whole cell lysate (20% of IP loaded) using ab264122 at 6 µl/mg lysate. Western blot was performed on the immunoprecipitate using a different antibody ab264423 at a 1/1000 dilution.
Lane 1: ab264122 IP in Ramos whole cell lysate.
Lane 2: Control IgG.Exposure time: 75 secs.
Datasheets and documents
References
ab264122 has not yet been referenced specifically in any publications.
