Product nameAnti-Ctip1/BCL-11A antibody
See all Ctip1/BCL-11A primary antibodies
DescriptionRabbit polyclonal to Ctip1/BCL-11A
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide aa 450-500. The exact sequence is proprietary.
Database link: Q9H165
- WB: Ramos and HEK-293T whole cell lysate. IP: Ramos whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264122 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000.|
FunctionFunctions as a myeloid and B-cell proto-oncogene. May play important roles in leukemogenesis and hematopoiesis. An essential factor in lymphopoiesis, is required for B-cell formation in fetal liver. May function as a modulator of the transcriptional repression activity of ARP1.
Tissue specificityExpressed at high levels in brain, spleen thymus, bone marrow and testis. Expressed in CD34-positive myeloid precursor cells, B-cells, monocytes and megakaryocytes. Expression is tightly regulated during B-cell development.
Involvement in diseaseNote=Chromosomal aberrations involving BCL11A may be a cause of lymphoid malignancies. Translocation t(2;14)(p13;q32.3) causes BCL11A deregulation and amplification.
Sequence similaritiesContains 6 C2H2-type zinc fingers.
modificationsSumoylated by SUMO1.
Cellular localizationCytoplasm. Nucleus. Associates with the nuclear body. Colocalizes with SUMO1 and SENP2 in nuclear speckles.
- Information by UniProt
- 2810047E18Rik antibody
- B cell CLL/lymphoma 11A (zinc finger protein) antibody
- B cell CLL/lymphoma 11A (zinc finger protein) isoform 2 antibody
All lanes : Anti-Ctip1/BCL-11A antibody (ab264122) at 0.1 µg/ml
Lane 1 : Ramos whole cell lysate at 50 µg
Lane 2 : Ramos whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : HEK-293T whole cell lysate at 50 µg
Developed using the ECL technique.
Exposure time: 3 minutes
Cells prepared using NETN lysis buffer.
Ctip1/BCL-11A was immunoprecipitated from 1mg Ramos whole cell lysate (20% of IP loaded) using ab264122 at 6 µl/mg lysate. Western blot was performed on the immunoprecipitate using a different antibody ab264423 at a 1/1000 dilution.
Lane 1: ab264122 IP in Ramos whole cell lysate.
Lane 2: Control IgG.
Exposure time: 75 secs.
ab264122 has not yet been referenced specifically in any publications.