Anti-Ctip1/BCL-11A antibody (ab264122)
Key features and details
- Rabbit polyclonal to Ctip1/BCL-11A
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Ctip1/BCL-11A antibody
See all Ctip1/BCL-11A primary antibodies -
Description
Rabbit polyclonal to Ctip1/BCL-11A -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide aa 450-500. The exact sequence is proprietary.
Database link: Q9H165 -
Positive control
- WB: Ramos and HEK-293T whole cell lysate. IP: Ramos whole cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab264122 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at 2-10 µg/mg of lysate. | |
| WB | 1/2000 - 1/10000. |
Target
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Function
Functions as a myeloid and B-cell proto-oncogene. May play important roles in leukemogenesis and hematopoiesis. An essential factor in lymphopoiesis, is required for B-cell formation in fetal liver. May function as a modulator of the transcriptional repression activity of ARP1. -
Tissue specificity
Expressed at high levels in brain, spleen thymus, bone marrow and testis. Expressed in CD34-positive myeloid precursor cells, B-cells, monocytes and megakaryocytes. Expression is tightly regulated during B-cell development. -
Involvement in disease
Note=Chromosomal aberrations involving BCL11A may be a cause of lymphoid malignancies. Translocation t(2;14)(p13;q32.3) causes BCL11A deregulation and amplification. -
Sequence similarities
Contains 6 C2H2-type zinc fingers. -
Post-translational
modificationsSumoylated by SUMO1. -
Cellular localization
Cytoplasm. Nucleus. Associates with the nuclear body. Colocalizes with SUMO1 and SENP2 in nuclear speckles. - Information by UniProt
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Database links
- Entrez Gene: 53335 Human
- Entrez Gene: 14025 Mouse
- Omim: 606557 Human
- SwissProt: Q9H165 Human
- SwissProt: Q9QYE3 Mouse
- Unigene: 370549 Human
- Unigene: 441102 Mouse
- Unigene: 471050 Mouse
see all -
Alternative names
- 2810047E18Rik antibody
- B cell CLL/lymphoma 11A (zinc finger protein) antibody
- B cell CLL/lymphoma 11A (zinc finger protein) isoform 2 antibody
see all
Images
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Western blot - Anti-Ctip1/BCL-11A antibody (ab264122)All lanes : Anti-Ctip1/BCL-11A antibody (ab264122) at 0.1 µg/ml
Lane 1 : Ramos whole cell lysate at 50 µg
Lane 2 : Ramos whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : HEK-293T whole cell lysate at 50 µg
Developed using the ECL technique.
Exposure time: 3 minutes4-8% SDS-PAGE.
Cells prepared using NETN lysis buffer.
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Immunoprecipitation - Anti-Ctip1/BCL-11A antibody (ab264122)Ctip1/BCL-11A was immunoprecipitated from 1mg Ramos whole cell lysate (20% of IP loaded) using ab264122 at 6 µl/mg lysate. Western blot was performed on the immunoprecipitate using a different antibody ab264423 at a 1/1000 dilution.
Lane 1: ab264122 IP in Ramos whole cell lysate.
Lane 2: Control IgG.Exposure time: 75 secs.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab264122 has not yet been referenced specifically in any publications.
