Recombinant Anti-Ctip1/BCL-11A antibody [EPR14943-44] (ab191401)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14943-44] to Ctip1/BCL-11A
- Suitable for: WB, ICC/IF, IHC-P, Flow Cyt (Intra), IP
- Knockout validated
- Reacts with: Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Ctip1/BCL-11A antibody [EPR14943-44]
See all Ctip1/BCL-11A primary antibodies -
Description
Rabbit monoclonal [EPR14943-44] to Ctip1/BCL-11A -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-P, Flow Cyt (Intra), IPmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Raji, Jurkat and 293 cell lysates; IHC-P: Human tonsil and rat spleen tissues; rat C6 cells. IP: Raji whole cell lysate Flow Cyt (intra): Jurkat cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14943-44 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab191401 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000 - 1/50000. Detects a band of approximately 120 kDa (predicted molecular weight: 91 kDa).
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ICC/IF |
1/450.
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IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Flow Cyt (Intra) |
1/200.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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IP |
1/50.
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Notes |
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WB
1/10000 - 1/50000. Detects a band of approximately 120 kDa (predicted molecular weight: 91 kDa). |
ICC/IF
1/450. |
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Flow Cyt (Intra)
1/200. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IP
1/50. |
Target
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Function
Functions as a myeloid and B-cell proto-oncogene. May play important roles in leukemogenesis and hematopoiesis. An essential factor in lymphopoiesis, is required for B-cell formation in fetal liver. May function as a modulator of the transcriptional repression activity of ARP1. -
Tissue specificity
Expressed at high levels in brain, spleen thymus, bone marrow and testis. Expressed in CD34-positive myeloid precursor cells, B-cells, monocytes and megakaryocytes. Expression is tightly regulated during B-cell development. -
Involvement in disease
Note=Chromosomal aberrations involving BCL11A may be a cause of lymphoid malignancies. Translocation t(2;14)(p13;q32.3) causes BCL11A deregulation and amplification. -
Sequence similarities
Contains 6 C2H2-type zinc fingers. -
Post-translational
modificationsSumoylated by SUMO1. -
Cellular localization
Cytoplasm. Nucleus. Associates with the nuclear body. Colocalizes with SUMO1 and SENP2 in nuclear speckles. - Information by UniProt
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Database links
- Entrez Gene: 53335 Human
- Entrez Gene: 14025 Mouse
- Entrez Gene: 305589 Rat
- Omim: 606557 Human
- SwissProt: Q9H165 Human
- SwissProt: Q9QYE3 Mouse
- Unigene: 370549 Human
- Unigene: 441102 Mouse
see all -
Alternative names
- 2810047E18Rik antibody
- B cell CLL/lymphoma 11A (zinc finger protein) antibody
- B cell CLL/lymphoma 11A (zinc finger protein) isoform 2 antibody
see all
Images
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Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: Empty
Lane 3: BCL11A (Ctip1) knockout HAP1 whole cell lysate (20 µg)
Lane 4: Raji whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab191401 observed at 91 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab191401 was shown to recognize BCL11A (Ctip1) in wild type cells as signal was lost at the expected MW in BCL11A (Ctip1) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and BCL11A (Ctip1) knockout samples were subjected to SDS-PAGE. Ab191401 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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ab191401 (purified) at 1/50 dilution (2.1 µg/mL) immunoprecipitating Ctip1/BCL-11A in Raji whole cell lysate.
Lane 1 (input): Raji(Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10µg
Lane 2 (+): ab191401 & Raji whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab191401 in Raji whole cell lysate
For western blotting, ab191401 at 1/500 dilution (2.1 µg/mL) and VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM /TBST . -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (ab191401)
Immunohistochemical analysis of paraffin-embedded, rat spleen tissue labeling Ctip1/BCL-11A with ab191401 at a 1/100 dilution (19 μg/ml). Counter stained with hematoxylin. In the negative control PBS was used instead of primary antibody.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunofluorescence analysis of, paraformaldehyde-fixed, rat C6 cells labeling Ctip1/BCL-11A with ab191401 at a 1/450 dilution (4 ug/ml). As secondary antibody goat anti-rabbit IgG (Alexa Fluor®488) ab150077 was used at a 1/200 dilution. In blue DAPI staining. In the negative controls cells were treated with anti-BCL11A at a 1/450 dilution as primary antibody and goat anti-mouse IgG (Alexa Fluor®594) at a 1/400 dilution as secondary antibody.
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Intracellular Flow Cytometry analysis of Jurkat (human acute T cell leukemia) labelling Ctip1/BCL-11A with purified ab191401 at 1/200 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Alexa Fluor® 488 goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
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All lanes : Anti-Ctip1/BCL-11A antibody [EPR14943-44] (ab191401) at 1/50000 dilution
Lane 1 : Raji cell lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 91 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (ab191401)
Immunohistochemical analysis of paraffin-embedded, Human tonsil tissue labeling Ctip1/BCL-11A with ab191401 at a 1/100 dilution (19 μg/ml). Counter stained with hematoxylin. In the negative control PBS was used instead of primary antibody.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Anti-Ctip1/BCL-11A antibody [EPR14943-44] (ab191401) at 1/50000 dilution + 293 cell lysate at 10 µg
Secondary
goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 91 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (2)
ab191401 has been referenced in 2 publications.
- Lazarus KA et al. BCL11A interacts with SOX2 to control the expression of epigenetic regulators in lung squamous carcinoma. Nat Commun 9:3327 (2018). PubMed: 30127402
- Liu N et al. Direct Promoter Repression by BCL11A Controls the Fetal to Adult Hemoglobin Switch. Cell 173:430-442.e17 (2018). PubMed: 29606353