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    ctip1bcl-11a-antibody-epr14943-44-ab191401.pdf

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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Acetylation
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Ctip1/BCL-11A antibody [EPR14943-44] (ab191401)

  • Datasheet
  • SDS
  • Certificate of Compliance
Submit a review Submit a question References (2)

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Western blot - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (ab191401)
  • Immunoprecipitation - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (ab191401)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (ab191401)
  • Immunocytochemistry/ Immunofluorescence - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (ab191401)
  • Flow Cytometry - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (ab191401)
  • Western blot - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (ab191401)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (ab191401)
  • Western blot - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (ab191401)
  • Anti-Ctip1/BCL-11A antibody [EPR14943-44] (ab191401)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14943-44] to Ctip1/BCL-11A
  • Suitable for: WB, ICC/IF, IHC-P, IP, Flow Cyt
  • Knockout validated
  • Reacts with: Rat, Human

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Overview

  • Product name

    Anti-Ctip1/BCL-11A antibody [EPR14943-44]
    See all Ctip1/BCL-11A primary antibodies
  • Description

    Rabbit monoclonal [EPR14943-44] to Ctip1/BCL-11A
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Rat
    IHC-P
    Rat
    Human
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Raji, Jurkat and 293 cell lysates; IHC-P: Human tonsil and rat spleen tissues; rat C6 cells. IP: Raji whole cell lysate Flow cyt: Jurkat cells.
  • General notes

     

     

    Previously labelled as Ctip1.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14943-44
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Neuroscience
    • Neurology process
    • Neural Signal Transduction
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • HAT

Associated products

  • Alternative Versions

    • Anti-Ctip1/BCL-11A antibody [EPR14943-44] - BSA and Azide free (ab242406)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Raji whole cell lysate (ab30124)
    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human Ctip1/BCL-11A protein (ab112415)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab191401 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC/IF
Rat
IHC-P
Rat
Human
IP
Human
WB
Human
All applications
Mouse
Application Abreviews Notes
WB
1/10000 - 1/50000. Detects a band of approximately 120 kDa (predicted molecular weight: 91 kDa).
ICC/IF
1/450.
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/50.
Flow Cyt
1/200.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Notes
WB
1/10000 - 1/50000. Detects a band of approximately 120 kDa (predicted molecular weight: 91 kDa).
ICC/IF
1/450.
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/50.
Flow Cyt
1/200.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Functions as a myeloid and B-cell proto-oncogene. May play important roles in leukemogenesis and hematopoiesis. An essential factor in lymphopoiesis, is required for B-cell formation in fetal liver. May function as a modulator of the transcriptional repression activity of ARP1.
  • Tissue specificity

    Expressed at high levels in brain, spleen thymus, bone marrow and testis. Expressed in CD34-positive myeloid precursor cells, B-cells, monocytes and megakaryocytes. Expression is tightly regulated during B-cell development.
  • Involvement in disease

    Note=Chromosomal aberrations involving BCL11A may be a cause of lymphoid malignancies. Translocation t(2;14)(p13;q32.3) causes BCL11A deregulation and amplification.
  • Sequence similarities

    Contains 6 C2H2-type zinc fingers.
  • Post-translational
    modifications

    Sumoylated by SUMO1.
  • Cellular localization

    Cytoplasm. Nucleus. Associates with the nuclear body. Colocalizes with SUMO1 and SENP2 in nuclear speckles.
  • Target information above from: UniProt accession Q9H165 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 53335 Human
    • Entrez Gene: 14025 Mouse
    • Entrez Gene: 305589 Rat
    • Omim: 606557 Human
    • SwissProt: Q9H165 Human
    • SwissProt: Q9QYE3 Mouse
    • Unigene: 370549 Human
    • Unigene: 441102 Mouse
    • Unigene: 471050 Mouse
    • Unigene: 53687 Mouse
    • Unigene: 88824 Mouse
    • Unigene: 16729 Rat
    see all
  • Alternative names

    • 2810047E18Rik antibody
    • B cell CLL/lymphoma 11A (zinc finger protein) antibody
    • B cell CLL/lymphoma 11A (zinc finger protein) isoform 2 antibody
    • B-cell CLL/lymphoma 11A antibody
    • B-cell lymphoma/leukemia 11A antibody
    • BC11A_HUMAN antibody
    • BCL-11A antibody
    • BCL11A antibody
    • BCL11A B cell CLL/lymphoma 11A (zinc finger protein) isoform 1 antibody
    • BCL11A L antibody
    • BCL11A S antibody
    • BCL11A XL antibody
    • BCL11a-M antibody
    • BCL11AL antibody
    • BCL11AS antibody
    • BCL11AXL antibody
    • C2H2 type zinc finger protein antibody
    • COUP TF interacting protein 1 antibody
    • COUP-TF-interacting protein 1 antibody
    • CTIP1 antibody
    • CTIP1, mouse, homolog of antibody
    • D930021L15Rik antibody
    • Ecotropic viral integration site 9 antibody
    • Ecotropic viral integration site 9 homolog antibody
    • Ecotropic viral integration site 9 protein antibody
    • Ecotropic viral integration site 9 protein homolog antibody
    • EVI-9 antibody
    • Evi9 antibody
    • Evi9, mouse, homolog of antibody
    • FLJ10173 antibody
    • FLJ34997 antibody
    • HBFQTL5 antibody
    • KIAA1809 antibody
    • mKIAA1809 antibody
    • OTTHUMP00000159788 antibody
    • OTTHUMP00000159789 antibody
    • OTTHUMP00000201250 antibody
    • OTTHUMP00000202084 antibody
    • Zinc finger protein 856 antibody
    • ZNF856 antibody
    see all

Images

  • Western blot - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (ab191401)
    Western blot - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (ab191401)

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: Empty
    Lane 3: BCL11A (Ctip1) knockout HAP1 whole cell lysate (20 µg)
    Lane 4: Raji whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab191401 observed at 91 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab191401 was shown to recognize BCL11A (Ctip1) in wild type cells as signal was lost at the expected MW in BCL11A (Ctip1) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and BCL11A (Ctip1) knockout samples were subjected to SDS-PAGE. Ab191401 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunoprecipitation - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (ab191401)
    Immunoprecipitation - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (ab191401)

    ab191401 (purified) at 1/50 dilution (2.1 µg/mL) immunoprecipitating Ctip1/BCL-11A in Raji whole cell lysate.
    Lane 1 (input): Raji(Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10µg
    Lane 2 (+): ab191401 & Raji whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab191401 in Raji whole cell lysate
    For western blotting, ab191401 at 1/500 dilution (2.1 µg/mL) and VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1000 dilution.
    Blocking and diluting buffer: 5% NFDM /TBST .

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (ab191401)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (ab191401)

    Immunohistochemical analysis of paraffin-embedded, rat spleen tissue labeling Ctip1/BCL-11A with ab191401 at a 1/100 dilution (19 μg/ml). Counter stained with hematoxylin. In the negative control PBS was used instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (ab191401)
    Immunocytochemistry/ Immunofluorescence - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (ab191401)

    Immunofluorescence analysis of, paraformaldehyde-fixed, rat C6 cells labeling Ctip1/BCL-11A with ab191401 at a 1/450 dilution (4 ug/ml). As secondary antibody goat anti-rabbit IgG (Alexa Fluor®488) ab150077 was used at a 1/200 dilution. In blue DAPI staining. In the negative controls cells were treated with anti-BCL11A at a 1/450 dilution as primary antibody and goat anti-mouse IgG (Alexa Fluor®594) at a 1/400 dilution as secondary antibody.

  • Flow Cytometry - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (ab191401)
    Flow Cytometry - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (ab191401)

    Flow Cytometry analysis of Jurkat (human acute T cell leukemia) labelling Ctip1/BCL-11A with purified ab191401 at 1/200 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Alexa Fluor® 488 goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

  • Western blot - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (ab191401)
    Western blot - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (ab191401)
    All lanes : Anti-Ctip1/BCL-11A antibody [EPR14943-44] (ab191401) at 1/50000 dilution

    Lane 1 : Raji cell lysate
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 91 kDa
    Observed band size: 120 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (ab191401)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (ab191401)

    Immunohistochemical analysis of paraffin-embedded, Human tonsil tissue labeling Ctip1/BCL-11A with ab191401 at a 1/100 dilution (19 μg/ml). Counter stained with hematoxylin. In the negative control PBS was used instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (ab191401)
    Western blot - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (ab191401)
    Anti-Ctip1/BCL-11A antibody [EPR14943-44] (ab191401) at 1/50000 dilution + 293 cell lysate at 10 µg

    Secondary
    goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 91 kDa
    Observed band size: 120 kDa why is the actual band size different from the predicted?

  • Anti-Ctip1/BCL-11A antibody [EPR14943-44] (ab191401)
    Anti-Ctip1/BCL-11A antibody [EPR14943-44] (ab191401)

Protocols

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (2)

Publishing research using ab191401? Please let us know so that we can cite the reference in this datasheet.

ab191401 has been referenced in 2 publications.

  • Lazarus KA  et al. BCL11A interacts with SOX2 to control the expression of epigenetic regulators in lung squamous carcinoma. Nat Commun 9:3327 (2018). PubMed: 30127402
  • Liu N  et al. Direct Promoter Repression by BCL11A Controls the Fetal to Adult Hemoglobin Switch. Cell 173:430-442.e17 (2018). PubMed: 29606353

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